GRADUATE COURSE IN ADVANCED SKELETAL MUSCLE METABOLISM
HHNS*6130 --- WINTER 2009
Dr. Arend Bonen, Rm. 330, ANNU Building: 824-4120 x53028 (office) x56654 (lab) abonen@uoguelph.ca FAX: 519-763-5902
Dr. Lawrence L. Spriet, Rm 334, ANNU Bldg; 824-4120 x53745 (office) x53907 (lab) lspriet@uoguelph.ca FAX: 519-763-5902
Students
Course designed for M.Sc. & Ph.D. students with a metabolic background.
Day and Time
Tuesday, 8:30 AM - 11:30 PM Room: ANNU 306
Course Outline and Objectives
This course is designed to examine selected areas and topics in contemporary human skeletal muscle metabolism at an advanced level. It is expected that the student is familiar with skeletal muscle metabolism to the depth provided by Newsholme and Leech’s 1983 Biochemistry for the Medical Sciences. The majority of learning will occur during discussions of state of the art research papers.
The course will focus on skeletal muscle but other organs/systems will be examined when relevant (liver, adipose tissue, circulation etc.). The emphasis will be on catabolic processes, but selected anabolic processes will also be addressed. Throughout the course we attempt to determine how the energy provision pathways meet the energy demands of the muscle cell during varying situations (rest, exercise, fasting, training/detraining) and how the body handles the various by-products of metabolism. Many of these topics will not be specifically addressed but are discussed during several of the weeks.
Course Format
The weekly format will consist of a discussion examining the 4-5 articles that have been assigned for the topic of the day. In some cases the instructor may take the final 30 minutes of a class to prepare for the discussion of the following week’s topic. If required, the instructors will also distribute a 1-2 page handout at the end of one class to set the stage for the next class. This should make the readings more meaningful. All students are expected to read all papers each week and any background reading they feel is necessary.
In addition, students will be asked to lead the discussions of the papers each week. Therefore, 4-5 students will be involved in leading the discussions each week. Depending on the number of students in the course, you can expect to lead a discussion every second or third week. The discussions are meant to be informal and do not require formal presentations. In the discussions we will examine points regarding the rationale, purpose and hypotheses of the study, the methods used, the obtained results and the interpretation of the findings. We ask that you also prepare a one-page synopsis of the paper you will discuss to be distributed to the other students.
Weekly Reading List
Background Texts
Newsholme, E.A., and C. Start. Regulation in Metabolism. Wiley, Toronto, 1973.
Houston , M.E. Biochemistry Primer for Exercise Science. 3rd Edition, Human Kinetics, Windsor, Ontario, 2006
Freinkel, N. (ed.). Contemporary Metabolism. Vol. 2. Plenum, New York, 1982.
Newsholme, EA, and AR Leech. Biochemistry for the Medical Sciences. Wiley, Toronto, 1983.
Peachey, L.D. (ed.) Skeletal Muscle. In: Handbook of Physiology, American Physiological Society, Williams and Wilkins, Baltimore, MD, 1984.
Wolfe, R.R. Tracers in Metabolic Research. Radioisotopes and Stable Isotope/Mass Spectrometry Methods. Alan R. Liss Inc., New York, 1984.
Beitner, R. Regulation of Carbohydrate Metabolism. Vol. I & II. CRC Press, Boca Raton, 1985.
Horton, E.S., and R.L. Terjung (eds.) Exercise, Nutrition, and Energy Metabolism. Collier Macmillan, Toronto, 1988.
Lehninger, A.L., D.L. Nelson, and M.M. Cox. Principles of Biochemistry. 2nd Edition, Worth Publishing, New York, 1993.
Hargreaves, M. Exercise Metabolism. Human Kinetics, Champaign, IL, 1995.
Hargreaves, M, and LL Spriet. Exercise Metabolism (2 nd Ed). Human Kinetics, Champaign, IL, 2006.
Rowell, L.B., and J.T. Shepherd (eds) Handbook of Physiology, American Physiological Society, Section 12: Exercise: Regulation and Integration of Multiple Systems. Oxford University Press, New York, 1996.
McGilvery, R.W. Biochemical Concepts. W.B. Saunders, Toronto.
Biochemistry of Exercise Series, Human Kinetics Publishers. Champaign, IL, Meetings V (Knuttgen, Vogel, and Poortmans, 1983), VI (Saltin, 1986), VII (Taylor, Gollnick, Green, Ianuzzo, Noble, Metivier, and Sutton, 1988), IX (Maughan and Shirreffs, 1996), and X (Hargreaves and Thompson, 1998).
Maughan, R., M. Gleeson, and P.L. Greenhaff. Biochemistry of Exercise & Training. Oxford Univ. Press, Oxford, 1997.
International Symposium on “Signalling in Muscle Metabolism”. Acta Physiologica Scandinavica 178:285-452, 2003.
Richter, E.A., B. Kiens, H. Galbo, and B. Saltin. Skeletal Muscle Metabolism in Exercise and Diabetes. In: Volume 441: Advances in Experimental Medicine and Biology. Plenum Press, New York, 1998.
D.R. Lamb and R. Murray (eds). The Metabolic Bases of Performance in Sport and Exercise. Human Kinetics, Windsor, Ontario, 1999. p. 1-51.
Journals with Articles Relevant to Substrate Metabolism
Acta Endocrinol.
Am. J. Clin. Nutr.
Am. J. Physiol. (Endocrinology & Metabolism; Cell Physiology; and Regulatory, Integrative & Comparative Sections)
Acta Physiol. Scand.
Applied Physiology, Nutrition and Metabolism (formerly Can. J. Appl. Physiol).
Biochim. Biophys. Acta
Biochem. J.
Can. J. Physiol. Pharmacol.
Can. J. Zoology
Cell
Diabetes
Diabetologica
Endocrinology
Eur. J. Appl. Physiol.
FASEB J.
Horm. Met. Res.
Int. J. Sports Med.
J. Appl. Physiol.
J. Biol. Chem.
J. Clin. Endocrin. Metab.
J. Clin. Invest.
J. Physiol. (London)
Metabolism
Med. Sci. Sports Exerc.
Pflugers Archiv.
Scand. J. Clin. Lab. Invest.
Reviews
Advances in Enzyme Regulation
Annual Review of Biochemistry
Annual Review of Physiology
Essays in Biochemistry
News in Physiological Sciences
Physiological Reviews
Recent Progress in Hormone Research
Trends in Biochemical Sciences
Student Evaluation
The workload will be heavy each week given the articles to read and the occasional need for background reading. Therefore, there are only two evaluations;
- Take-home Exam (50%) - Exam will be picked up after the last class preceding the winter break week. The exam is to be done in 48 hr. The exam will have 6-8 questions covering the topics from the first half of the course and you will be required to answer 2 questions. Only 1 question per section (week) can be answered.
- Final Exam (50%) - Exam will be take home and returned in 48 hr. Pick up after the final class or during the following week. The exam will require you to answer two questions from 6-8 options covering the topics from the last half of the course .