Mr. Robert (Bob) Harris, Manager
bharris@uoguelph.ca
Office: 1255 Science Complex
Telephone: 519-824-4120
office x 56409;
lab x58962
Dr. Robert Mullen
Faculty Advisor
rtmullen@uoguelph.ca
Office: 4470 Science Complex
Telephone: 519-824-4120 office x 56479
The Electron Microscopy (EM) unit is part of the Microscopy Imaging Facility (formerly the Guelph Regional Integrated Imaging Facility [GRIIF]) and located within the University of Guelph Advanced Analysis Center (AAC), a multi-instrument facility that focuses on X-ray crystallography, NMR, mass spectrometry, gene sequencing and microarray analyses, as well as confocal and electron microscopy.
Open on a cost recovery basis to both Canadian and foreign researchers, the EM Unit consists of three electron microscopes (TEMs) and an array of ancillary preparatory equipment. Each of the TEMs fills a unique niche in the facility; specific details on each of these instruments are provided below.
Specifications on TEMs in the Facility
Philips CM10:
Includes an Edax Sapphire x-ray detector for performing spot elemental analyses and a top mount SIS/Olympus Morada 11 megapixel CCD camera that offers a wide field of view for imaging plant and animal tissues and whole cells. This instrument is located in Room 1218 in the Science Complex.
LEO 912ab
An Energy Filtered TEM (EFTEM) excellent for imaging material science and nanotechnology samples, as well as biological molecules. An in-column Omega energy filter makes this TEM very useful for acquiring images from low contrast biological and material specimens in zero loss mode. The energy filter also enables Electron Spectroscopic Imaging (ESI) and Electron Energy Loss Spectroscopy (EELS). This TEM is also fitted with a bottom mount SIS/ Olympus Cantega 2K digital camera. It is also anticipated that the instrument will be available for room temperature Electron Tomography once the appropriate software is installed. Located in Room 1209 in the Science Complex
FEI Tecnai G2 F20
A 200kV field emission TEM that is reserved exclusively for cryo-temperature, low dose operation. Samples are prepared by flash freezing in our FEI Vitrobot, Leica High Pressure Freezer or Leica Cryo-Microtome and maintained at temperatures below Ð170oC while being examined. A suite of software applications are available including those for tomography and collecting cryo-temperature tilt series. The precise nature of the specimen stage allows for quick scanning of specimen grids and exact recall of areas of interest - especially valuable to researchers collecting large data sets for Single Particle Analysis. This TEM is also fitted with a bottom mount Gatan 4k CCD. Located in Room 1216 in the Science Complex.
Fees
The EM Unit is open to anyone at the University of Guelph, within Canada, or elsewhere in the world interested in electron microscopy. Billing is on a pay-for-use basis, unless arranged otherwise.
User Fees
| On Campus Users | Off Campus Users | |
|---|---|---|
| CM10 (routine TEM) | $50.00/hr | $80.00/hr |
| CM10/EDS | $50.00/hr | $80.00/hr |
| LEO 912 AB | $50.00/hr | $80.00/hr |
| CRYOTECNAI G2 F20 | $60.00/hr | $100.00/hr |
| Sectioning/Staining | $50.00/hr | $80.00/hr |
| Darkroom and image processing | $50.00/hr | $80.00/hr |
| On Campus Users | Off Campus Users | |
|---|---|---|
| Embedding | $50.00/sample | $80.00/sample |
| Immuno-labeling (plus embedding charge) | $50.00/sample | $120.00/sample |
| Freeze Substitution | $80.00/sample | $125.00/sample |
- Cryo-sectioning may be offered as a service in the near future; contact Mr. Harris for details.
- Consumables such as biological markers and probes, special chemicals, photo film and paper, etc. will be billed according to use.
Computer Support
Computational power for constructing three-dimensional images from large data sets acquired from the EM Unit can be easily accessed at the University of Guelph via SHARCNET - a consortium of colleges and universities linking clusters of high performance computers that can be used free
User Information
Please contact the EM Unit manager, Mr. Robert (Bob) Harris by e-mail (preferred) or by phone, if you are interested in using the facility.