|
M
o
l
l
u
s
c
M
o
l
e
c
u
l
a
r
N
e
w
s
|
Add a primer to the list of primers.
All boxes must be filled in.
Do you need help or an example?
Remember to also submit information on PCR conditions and products for this primer.
Please give the sequences 5' to 3' leaving a space every three nucleotides and using lower case letters (g, a, t, c, i = inosine). For degenerate primers use parentheses with commas between the alternative nucleotides for that position. For example agt c(a,t)g would mean a mixture of 50% agt cag and 50% agt ctg. See the example:
Primers
Back to the form
- 4 lines of data.
- Text boxes marked with an asterisk (*) must be filled in.
- "Max Chars" is the maximum number of characters in each line of data.
|
Row | Max Chars | Example |
| Primer name | (40) * | 12sa-L |
| Designer name | (40) * | Kocher |
| Sequence | (80) * | aaa ctg gga tta gat acc cca cta t |
| Publication | (160) * | Kocher 1989. Proc. Natl. Acad. Sci., U.S.A. 86:6196-6200. |
- The name should be exactly the one used for the primer in original publication (up to 40 characters).
- Type the surname only of the person who designed/first published the primer (up to 40 characters).
- The primer sequence (up to 80 characters). Please give the sequences 5' to 3' leaving a space every three nucleotides and using lower case letters (g, a, t, c, i = inosine). For degenerate primers use parentheses with commas between the alternative nucleotides for that position. For example agt c(a,t)g would mean a mixture of 50% agt cag and 50% agt ctg.
- Literature citation of the original publication in the format used by Nature, but omitting all authors names except the surname of the first author. (up to 160 characters).