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MBG*4300 Plant Molecular Genetics

UNDERGRADUATEMolecular genetics of plants. Topics include: plant genome diversity and synteny; Arabidopsis thaliana genome, hormonal, environmental and developmental regulation of gene expression; chloroplast and mitochondrial genomes; and gene expression and silencing in transgenics. The course will be delivered using a lecture and paper discussion format. Students will learn and use a variety of computer techniques to search and analyze plant genome databases.

Instructor

M. Raizada
Rm. 406 Crop Science Building
Phone: ext. 53396
Email: raizada@uoguelph.ca

Prerequisites

MBG*2020

Lecture & Lab Hours

Lecture/Discussions: Tues, Thurs 8:30am
However, the class will be organized into small groups, so one of the lectures will be a group discussion, time to be organized.

Course Content

Overall Theme: Plants are immobile, autotrophic and multicellular: How do genes and gene regulation make immobility possible? How are plants and plant molecular genetics unique compared to animals (mobile, heterotrophic), microbes (unicellular) or fungi (immobile, heterotrophic)?

Format: The format of the course has been changed from last year. Now, primary learning will be to solve problems in plant molecular genetics using independent problem-based learning as part by an initiative by Alastair Summerlee. Students will learn about the practice of being a critical scientist, which involves understanding what is truly known and unknown, writing and defending a grant proposal and peer reviewing the research/proposals of others. There will be a 3-4 week cycle:

Week 1: In a small group, discuss a great problem in plant molecular genetics, then learn independently about an aspect of the problem (equivalent to a review).

Week 2: Orally present and discuss your findings/review in a small group.

Week 3: Present written and oral grant proposal to peer reviewers.

Week 4: Peer reviewers present critical oral and written report on proposals.

Topics: The course will rely heavily on whole genome/global expression profile analyses and introduction to relevant online databases. Topics may include:

  • plant proteins and proteomics
  • evolution of plant proteins and plant genomes
  • coordination of nuclear and chloroplast genomes
  • plant gene regulation
  • molecular genetics of primary and secondary metabolism
  • biotic and abiotic stress resistance
  • plant development

Practical Knowledge & Skills

One session/week will be devoted to practical skills learning, much of it computer-based. Topics will include:

  • how to read a paper critically,
  • journal impact factors
  • online databases tutorials (protein, comparative genomics, etc.)
  • lifelong learning
  • online molecular software,
  • molecular protocols
  • patent databases
  • career mentoring
  • graduate schools
  • applying for jobs

Evaluation of Student Progress

3 short oral reports/discussion – each 10%
3 short written grant proposals – each 10%
3 short peer-review reports –each 10%
1 molecular/genetics database assignment as part of the practical skills training of the course – 10%

Your peers provide part of the evaluation.

NO FINAL EXAM