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PBIO*4750 Genetic Engineering of Plants

UNDERGRADUATE An examination and discussion of the principles, protocols and applications of molecular biology and transformation technology to the genetic improvements of plants.

Instructor

TBA

NOTE: the course information listed below is for past course instructions and will change with the new lecturer.

Course Objectives

The objective of this course is to introduce students to the basic biological concepts, methodologies and issues associated with transgenic crop plants. Techniques for genetically engineering plants are presented in the context of case studies consisting of prominent examples of transgenic crop plants that have come and gone from the agrifood sector, are in current use or in some stage of development. Each case study represents a unique application of this technology and provides a real-life framework for discussion of related socioeconomic, environmental, ethical and health issues. Discussion papers are used to complement the case studies.

Prerequisites

MBG*2020

Lecture Hours

Lecture: M, W, F: 1:30-2:30, Crop Science 403
Tutorial/Discussion: M: 2:30-5:30, Crop Science 403

Reference Texts

- HS Chawla. Introduction to Plant Biotechnology. 2000. Science Publishers Inc.
- KM Oksman-Caldentey and W Barz. Plant Biotechnology and Transgenic Plants.

Course Content & Schedule


1. General Overview of Transgenic Plants(H)
2. Case Study #1: Genetic Engineering of Herbicide Resistance: Roundup Ready Soybeans
  Lectures:
 
  • Overview of soybean crop/utilization(H)
  • Herbicides in agriculture and herbicide tolerance in plants(H)
    • pathways and enzyme targets of major herbicide
  • Gene transfer by particle bombardment(H)
  • RR vector and gene integration (H)
  • Environmental issues associated with herbicide resistant crop plants(H)
  • Case Study Paper:
    • Development, identification, and characterization of a glyphosate-tolerant soybean line. 1995. Padgette et al. Crop Sci. 35:1451-61
  • Other References:
    • Predicted impact of transgenic, herbicide tolerant corn on drinking water quality in vulnerable watersheds of the mid-western USA.Wauchope et al. 2002, Pest Manag. Science 58(2): 146-60
  • Quiz 1  Jan 23
3. Case Study #2: Transgenic Plants Resistant to Insects: Bt Corn
  Lectures:
 
  • Insect damage to world crops and strategies for insect resistance in plants(H)
  • History and biology of Bacillus thuringiensis (Bt) and insecticidal proteins(H)
  • Plant transformation vectors : major regulatory features(H)
  • Analysis of transgenic plants I: PCR, Southern blots (H)
  • Environmental issues associated with Bt crops(H)
  • Case Study Paper:
    • Field performance of elite transgenic maize plants expressing an insecticidal protein derived from Bacillus thuringiensis. 1993. Koziel et al. Bio/Technology (Nature Biotechnology)11:194-9
  • Other References:
    • Field tests on managing resistance to Bt-engineered plants. Shelton et al. Nat. Biotech 2000, 18(3) pp. 339-42.
    • Impact of Bt pollen on monarch butterfly populations: A risk assessment. 2001. Sears et al. Proc Nat Acad Sci 98(21):11937-42
    • Effectiveness of border areas in confining the spread of transgenic Brassica napus pollen. 2002. Staniland et al. Can J Pl Sci 80:521-6
  • Quiz 2 Feb 8
4. Case Study #3: Engineering Food Crops for Quality: Flavr Savr Tomato and Golden Rice
  Lectures:
 
  • Plant transformation by Agrobacterium(H)
  • Plant tissue and regeneration systems for transformation(H)
  • Feed and food safety issues associated with transgenic crops(H)
  • Case Study Papers:
    • Reduction of polygalacturonase activity in tomato fruit by antisense RNA”. 1988. Sheehy et al.  Proc Nat Acad Sci (USA) 85:8805
    • Engineering the provitamin A (B-carotene) biosynthetic pathway into (carotenoid-free) rice endosperm. 2000. Ye et al.  Science, 287:303
  • Other References:
    • Clinical risk assessment of GM foods. 2002. Lack. Toxicol Lett 127:337-40
  • Quiz 3 Mar 5
5. Case Study #4: Genetically engineered pollination control systems
  Lectures:
 
  • Hybrid crop systems(H)
  • Analysis of transgenic plants II: RT-PCR, Northern, Western blots, ELISA
  • Case Study Paper:
    • Induction of male sterility in plants by chimeric ribonuclease gene. 1990. Mariani et al.  Nature 347:737-41
  • Quiz 4 Mar 19
6. Case Study #5: Molecular Farming of Plants for Applications in Veterinary and Human Medicine
  Lectures:
 
  • General strategies and targets: antigens, antibodies, growth factors, antibiotic peptides(H)
  • Case Study Papers:
    • Rapid production of specific vaccines for lymphoma by expression of the tumor-derived single-chain fv epitopes in tobacco plants. 1999. McCormick et al. Proc Nat Acad Sci USA. 96:703-08.
    • High-yield production of a human therapeutic protein in tobacco chloroplasts. 2000. Staub et al. Nat Biotech 18:333-8
  • Other References:
    • Boosting heterologous protein production in transgenic dicotyledonous seeds using Phaseolus vulgaris regulatory sequences. 2002 De Jaegeri et al. Nat Biotech 20(12):1265-8
  • Quiz 5 April 2
7. Review and last class April 4
8. Final Exam: April 10, 7:00 - 10:00

Evaluation of Student Progress

Quizzes (5): 50%
Final Exam: 50%

The quizzes are cumulative in that the questions may draw on information presented in previous case studies.