Protocols













General Protocols Sites

Double Twist (protocols and Methods Discussion Forums)
Molecular Biology Protocols (protocols by subject from various labs around the world at Double Twist)
Comprehensive Protocol Collection (from Dartmouth University)
Molecular Biology Protocols (US Dept Commerce/NOAA/NMFS/NWFSC)
BioMedNet (technical tips and research tools)

National Diagnostics (various protocols)

Common Protocols

Company Addresses
Laboratory Safety
Media
Buffers
Phosphate Buffers
Stock Solutions
Antibiotics
Culture Collection
Collecting Cotton Tissue
Tissue Lyophilization
Genomic DNA Purification
Cotton DNA Isolation
Arabidopsis DNA Purification from Nuclei
Plant DNA Extraction Protocol - Meyerowitz Laboratory
Plant DNA Extraction Protocol - PMB
Plant Nuclei DNA Extraction Protocol - DP
Plant Mitochondrial DNA Extraction Protocol - DP
Plant Chloroplast DNA Extraction Protocol - DP
Isolation of Genomic DNA from Azorhizobium caulinodans ORS571
DNA Extraction from ORS571
DNA Extraction Procedure for Rhizobium spp.
General DNA Extraction Procedure for Bacteria
ReadyAmp™ Genomic DNA Purification System
Quiagen Preps
Lessons In Paranoia
RNA Preparation Methods
RNA Extraction from Cotton
Plant RNA from Potato
Isolation Of RNA From Bacteroids and Bacteria
Transformation Competent Cells
Transformation
One-step Transformation of E. coli
Transformation of Yeast - LiAc Method
X-Gal
Large Scale Isolation of Plasmid and Bacterial DNA
Alkaline Lysis DNA Purifications from Bacteria
High-Copy Plasmid Purfication Using The TL-100 Rotor
Xanthomonas campestris Plasmid DNA Mini-Prep
Plasmid Isolation - Medium
Plasmid Isolation - Intermediate
Plasmid Isolation - Large
Modified Eckhardt Gel Procedure
DE52 DEAE Cellulose Phage Lambda DNA Purification Maxi-Prep
Phage Lambda DNA Purification Maxi-Prep
TL-100 Phage Purification
TL-100 Phage Rapid Purification Protocol
P1 DNA preparation
P1 DNA PEG precipitation
Fungal DNA Isolation
Single-Fly DNA Preps for PCR
Cesium Chloride Gradients
Restriction Enzyme Buffer Recipes
Restriction Enzyme Buffer Mixes
Restriction Enzyme Digest
Agarose Gel Electrophoresis
Molecular Weight Standards
Methylene Blue DNA Staining
Glassmilk DNA Purification
DNA from Low Melting Temperature Agarose
Precipitating DNA
Quantifying DNA
Dephosphorylating DNA
PCR
Cloning Blunt-end PCR Fragments
TA Subloning of PCR Fragments
PCR site-directed mutagenesis
Subloning of PCR Fragments
RT-PCR
Differential Display-Reverse Transcription-PCR
Optimized Welsh DDRT-PCR Protocol
DDRT-PCR Eluting Bands
Microplate Templates
PCR Error Rates
Differential cDNA Screening Procedures
Southern Transfers
Southern Transfer - Sponge Method
Hybridizations
Hybridization Conditions - Northerns
Post Hybridization
DNA Probes
Radioactive Labelling of DNA
Randon Primer Oligo-labelling of DNA
Random Primer Method
Spin Columns
PCR Protocol
PCR Amplification from Microbial Colonies
RAPDs
Extra Long PCR (Church Lab)
Sequencing Protocols
Exonuclease Protocols
Misc.
Mutagenesis
Equilibrating Phenol
Siliconizing Glassware
Preparing Salmon Sperm DNA
Cleaning Radiation
Clean-up for Ethidium Bromide
Removal of CsCl/EtBr Waste
Arabidopsis Seed Collection
Arabidopsis Transformation
Arabidopsis Vacuum Infiltration
Preparation of DNA from Single Rice Seedlings
Isolation of RNA and DNA from Rice Seedling Mitochondria
A simple screning method for transgenic rice tissue based on PCR
Non-aqueous Method for Purification of Cytoplasmic DNA from Individual Rice Plants for RFLP Analysis
Enhancement of Division Frequency of Protoplasts and Plant Regeneration in Rice
Isolation of RNA and DNA from different rice tissues
Improved histochemical staining for beta-D-glucuronidase activity in monocotyledonous plants
A rapid, reliable method for preparing somatic chromosomes of rice
cDNA Topics
Misc. Methods (DUMP FILE)
Misc. Stocks
Tissue Culture Stocks
Misc. Strains
A. Thaliana Topics
Preparation Of Nylon Filters For Dot-blot DNA Hybridization
Xanthomonas campestris Tri-Parental Mating Protocol
Agrobacterium-mediated Transformation of Cotton
MOPS Minimal Medium for Xanthomonas campestris
Xanthomodadin Extraction from Xanthomonas campestris
Bacteroid Isolation
Quick Test For Restriction Enzymes



DNA Analysis
- RNA Analysis - Protein Analysis - Gene Structure Analysis - Statisitcal Analysis - Protocols - Transgenics

Electronic Journals - Genomics - Databases -
Dept. of Plant Agriculture - University of Guelph - U of G Library