WJ Brad Hanna, DVM, PhD
Ontario Veterinary College
University of Guelph
Home__
Research

Heritable neuromuscular diseases of domestic animals

Current Projects

Project Collaborators Support
Bovine spastic syndrome Dr Dennis Bulman, Molecular Medicine, Ottawa Health Research Institute
Dr John Baird, Clinical Studies, U Guelph
Dr Jim Squires & Dr Flavio Schenkel, OAC, U Guelph
Canadian Dairy Network/NSERC #CRDPJ 417374-11 to WJBH et al. (2011-2015)
Bovine spastic syndrome Dr Dennis Bulman, Department of Pediatrics, U Ottawa
Dr Flavio Schenkel & Dr Mehdi Sargolzaei, OAC, U Guelph
Dr John Baird, Clinical Studies, U Guelph
Pending

Graduate students

Wes Rose, BSc (Guelph)
Scoping review and systematic review of veterinary acupuncture.
MSc candidate - GRADUATED 2016

Erin Preiss, DVM (Guelph)
T wave and QT interval in the standardbred horse: A descriptive study.
MSc candidate - GRADUATED 2016

Anna Neustaeter, BSc [co-advisor]
Genome-wide association study of spastic syndrome in Holstein cattle.
MSc candidate - GRADUATED 2015

Mike Chouster, BSc (Toronto), BSc (Concordia)
Linkage analysis of spastic syndrome in Holstein cattle.
MBS candidate - GRADUATED 2012

Kristin Mueller, BSc (Guelph)
Sequence analysis of cardiac ion channels in standardbred horses with lone atrial fibrillation.
MSc candidate - GRADUATED 2009

Cory Todd, BSc (Guelph)
Hereditary myotonia in Maltese dogs.
MSc candidate - GRADUATED 2008

Dan Finnigan, BSc
Investigation of CLCN1 as a candidate gene conferring Myotonia Hereditaria in two dogs.
MSc candidate - GRADUATED 2005

Lee Ellis, BSc (Guelph)
Altered slow inactivation in sodium channels containing the equine hyperkalemic periodic paralysis mutation.
MSc candidate - GRADUATED 2002

Publications

Peer-reviewed publications

15) Sanchez A, Valverde A, Sinclair M, Mosley C, Singh A, Mutsaers AJ, Hanna B, Johnson R, Gu Y, Beaudoin-Kimble M (2016).  Evaluation of the antihistaminic and cardio-respiratory effects of diphenhydramine in anesthetized dogs undergoing excision of mast cell tumors.  Journal of the American Veterinary Medical Association (accepted May 2016).

14) Sanchez A, Valverde A, Sinclair M, Mosley C, Singh A, Mutsaers A, Hanna B, Gu Y, Johnson R (2016).  The pharmacokinetics of DPH after the administration of a single intravenous or intramuscular dose in healthy dogs.  Journal of Veterinary Pharmacology and Therapeutics, Jan 27 (Epub ahead of print).

13) Grane-Bolderas N, Spring C, Hanna WJB, Pastor-Anglada M, Coe IR. Novel Nuclear hENT2 Isoforms Regulate Cell Cycle Progression via Controlling Nucleoside Transport. Cellular and Molecular Life Sciences (accepted July 2016).

12) Nivillac NMI, Wasal K, Villani DF, Naydenova Z, Hanna WJB, Coe IR (2009). Disrupted plasma membrane localization and loss of function reveal regions of human equilibrative nucleoside transporter 1 involved in structural integrity and activity. Biochimica et Biophysica Acta - Biomembranes 1788:2326-2334.

11) Cribb NC, Bouré LP, Hanna WJB, Akens MK, Mattson SE, Monteith GJ, Weese JS (2009). In vitro and in vivo evaluation of ferric-hyaluronate implants for delivery of amikacin sulfate to the tarsocrural joint of horses. Veterinary Surgery 38:498-505.

10) Finnigan DF, Hanna WJB, Poma R, Bendall AJ (2007). A novel mutation in the CLCN1 gene associated with Myotonia Hereditaria in an Australian Cattle Dog. Journal of Veterinary Internal Medicine 21:458-463.

9) Machado J, Abdulla P, Hanna WJB, Hilliker AJ, Coe IR (2007). Genomic analysis of nucleoside transporters in Diptera and functional characterization of DmENT2, a Drosophila equilibrative nucleoside transporter. Physiological Genomics 28:337-347.

8) McGurrin MKJ, Physick-Sheard PW, Kenney DG, Kerr C & Hanna WJB (2005). Transvenous electrical cardioversion of equine atrial fibrillation: technical considerations. Journal of Veterinary Internal Medicine 19:695-702.

7) McGurrin MK, Physick-Sheard PW, Kenney DG, Kerr C, Hanna WJB, Teixeira F & Weese JS (2003). Transvenous electrical cardioversion in equine atrial fibrillation: technique and successful treatment of three horses. Journal of Veterinary Internal Medicine 17:715-718.

6) Prescott JF, Hanna WJB, Reid-Smith R & Drost K (2002). Antimicrobial drug use and resistance in dogs. Canadian Veterinary Journal 43:107-116.

5) Renninger GH, Lajoie C, Hanna WJB, Fong D, House C & Zelin J (1997). Phase-shifting and entrainment of a circadian rhythm in Limulus polyphemus by ocular and extraocular photoreceptors. Biological Rhythm Research 28:50-68.

4) Hanna WJB, Tsushima RG, Sah R, McCutcheon LJ, Marban, E & Backx PH (1996). The equine periodic paralysis Na+ channel mutation alters molecular transitions between the open and inactivated states. Journal of Physiology (London) 497:349-364.

3) Hanna WJB, Johnson E, Chaves D & Renninger GH (1993). Photoreceptor cells dissociated from the compound lateral eye of the horseshoe crab, Limulus polyphemus II: Function. Visual Neuroscience 10:609-620.

2) Jinks RN, Hanna WJB, Renninger GH & Chamberlain SC (1993). Photoreceptor cells dissociated from the compound lateral eye of the horseshoe crab, Limulus polyphemus I: Structure and ultrastructure. Visual Neuroscience 10:597-607.

1) Hanna WJB, Horne JA & Renninger GH (1988). Circadian photoreceptor organs in Limulus II: the telson. Journal of Comparative Physiology A 162:133-140.

Conference Proceedings and Abstracts

33) Preiss E, Hanna WJB, Physick-Sheard PW (2016). T-wave and QT interval in the Standardbred horse: a descriptive study. 67th Annual Meeting of the European Federation of Animal Science, Belfast, UK, Aug 29 – Sep 2, 2016.

32) Sanchez A, Valverde A, Sinclair M, Mosley C, Singh A, Mutsaers AJ, Hanna B, Johnson R, Gu Y, Beaudoin-Kimble M. Evaluation of the antihistaminic and cardio-respiratory effects of diphenhydramine in anesthetized dogs undergoing excision of mast cell tumors. 12th World Congress of Veterinary Anaesthesiology, Kyoto, Japan, Sep 2015.

31) Valverde A, Sanchez A, Johnson R, Sinclair M, Mosley C, Hanna B, Singh A, Mutsaers T (2014). Determination of the pharmacokinetics of diphenhydramine in dogs. Association of Veterinary Anaesthetists Spring Meeting, 24th - 25th April, Nottingham, UK.

30) Rose WJ, Sargeant JM, Kelton DF, Hanna WJB (2014). A scoping review of the evidence for efficacy of acupuncture in companion animals. EBVM2014: 1st International Evidence-Based Veterinary Medicine Network Conference, 23rd - 24th October, Windsor, UK.

29) Hanna WJB (2013). Critical Thinking Series. Evidence-Based Veterinary Medicine Association Leadership Conference. On-line, Aug 9.

28) Neustaeter A, Schenkel F, Bulman D, Baird J, Hanna WJB (2013). Genetic analysis of spastic syndrome in Holstein cattle. Fall meeting of the Canadian Dairy Network, Nov 4.

27) Grane-Boladeras N, Hanna WJB, Coe IR, Pastor-Anglada M (2013). Identification and characterization of 3 novel nuclear splice variants of the human Equilibrative Nucleoside Transporter 2 (hENT2). Annual Meeting of the American Society for Cell Biology, New Orleans, LA, Dec 14-18.

26) Chouster M, Ventura R, Hanna B, Baird J, Bulman D, Schenkel F (2012). A preliminary Genome Wide Association Study to identify candidate genes responsible for Spastic Syndrome in Holstein cattle. Canadian Dairy Network - Dairy Cattle Breeding and Genetics Committee Meeting, September 18, Guelph, Ontario.

25) Arendse S, Poma R, MacLusky N, Hanna B (2009). Evaluation of short-term electroencephaolographic recording in dogs diagnosed with idiopathic epilepsy. Proceedings of the Canadian Society for Life Science Research (CSLSR). November 13-14; Ottawa, Ontario.

24) Nivillac NMI, Villani DF, Wasal K, Naydenova Z, Hanna WJB, Coe IR (2008). The C-terminus of human Equilibrative Nucleoside Transporter 1 is involved in correct localization and function. Gordon Research Conference: Membrane Transport Proteins. July 20-25, Lucca, Italy.

23) Binnington J, Hanna WJB, Kalisch B (2006). Effects of nerve growth factor and nitric oxide synthase inhibitors on amyloid precursor protein mRNA and protein expression. Proceedings of the Annual Meeting of the Society for Neuroscience, Atlanta, USA.

22) Folkl A, Hanna WJB, Poma R, Bendall AJ (2006). Development of a DNA-Based Blood Test for the CLCN1 Mutation Associated with Hereditary Myotonia in Australian Cattle Dogs. Pet Trust Forum, University of Guelph, Guelph, Ontario.

21) Cribb N, Bouré LP, Weese JS, Akens MK, Hanna WJB, Mattson S (2005). Development of amikacin-impregnated hyaluronan implants for the treatment of septic arthritis in horses. Oral presentation at the Resident Forum, 40th Annual meeting of the American College of Veterinary Surgeons, San Diego, USA. (Published in Veterinary Surgery, 2005, Vol. 34 p. E5.)

20) Lee C, Hanna WJB, Woolley GA, Yoo D (2005). PRRS virus small envelope (E) protein as a potential viroporin required for uncoating and replication. Proceedings of the 85th Conference of Research Workers in Animal Diseases (CRWAD), December 4-6, St Louis, Missouri, USA.

19) Lee C, Hanna WJB, Woolley GA, Yoo D (2005). PRRS virus small envelope (E) protein as a potential viroporin required for uncoating and replication. International PRRS Symposium, December 3, St Louis, Missouri, USA.

18) Finnigan DF, Da Costa R, Parent JM, Hanna WJB, Bendall AJ (2005). A novel form of canine inherited myotonia. Society for Neuroscience Abstracts, November 12-17, Washington, D.C., USA.

17) Finnigan D, Da Costa R, Parent JM, Hanna WJB, Bendall AJ (2005). A novel form of canine inherited myotonia. Proceedings of the Southern Ontario Neuroscience Association meeting, June 3, McMaster University, Hamilton, Ontario.

16) Finnigan D, Da Costa R, Parent JM, Hanna WJB, Bendall AJ (2005). A novel mutation in canine inherited myotonia. Pet Trust Forum, University of Guelph, Guelph, Ontario, April 23.

15) Grovum WL, Ellison BR, Bignell WW, Harris WH & Hanna WJB (2003). Preliminary data on the physiological significance of cholecystokinin (CCK) as a signal of satiety in pigs. Proceedings of the 9th International Symposium on Digestive Physiology in Pigs; May 14-17, Banff, Alberta.

14) Ellis LD, Staempfli HR & Hanna WJB (2002). Altered slow inactivation in sodium channels containing the equine hyperkalemic periodic paralysis (HyperPP) mutation. Proceedings of the 32nd Annual Meeting of the Society for Neuroscience (Vol 28): poster 834.17, November 2-7, Orlando, Florida, USA.

13) Hoffman EP, Wu FF, Ellis LD & Hanna WJB (2001). Muscle ion channelopathies. Proceedings of the International Ion Channel Conference: Channelopathies 2001; July 1-3, Sheffield, UK.

12) Ellis LD, Staempfli HR & Hanna WJB (2001). Enhanced slow inactivation in sodium channels containing the equine hyperkalemic periodic paralysis (HyperPP) mutation. Proceedings of the Southern Ontario Neuroscience Association Meeting 21:10; June 1, McMaster University, Hamilton, Ontario.

11) Hanna WJB, Chaves D, Wu FF, Hoffman EP & Staempfli HR (2000). Effects of selected anticonvulsants on sodium channels containing the equine hyperkalemic periodic paralysis (HPP) mutation. Proceedings of the 30th Annual Meeting of the Society for Neuroscience (Vol. 26): poster 713.16, November 4-9, New Orleans, Louisiana, USA.

10) Prescott JF, Hanna B & Reid-Smith R (2000). Antimicrobial drug use and resistance in companion animals. Australian Veterinary Association Annual Conference Proceedings, Perth.

9) Hanna WJB, Tsushima RG & Backx PH (1995). Modelling implicates a specific sodium channel transition defect in equine periodic paralysis. Society for Neuroscience Abstracts 21:1817.

8) Hanna WJB, McCutcheon LJ, Staempfli HR, Marban E & Backx PH (1994). The equine periodic paralysis mutation alters sodium channel inactivation. Journal of General Physiology 104:17a-18a.

7) Hanna WJB, McCutcheon LJ, Staempfli HR, Marban E & Backx PH (1994). Defective inactivation in sodium channels containing the equine periodic paralysis mutation. Society for Neuroscience Abstracts 20:66.

6) Hanna WJB, McCutcheon LJ, Staempfli HR & Backx PH (1994). The equine periodic paralysis mutation disrupts skeletal muscle sodium channel inactivation. Swiss Equine Veterinary Association Meeting, Interlaken, Switzerland.

5) Hanna WJB, McCutcheon LJ, Staempfli HR & Backx PH (1994). Use of the Xenopus ion channel expression system in the investigation of hereditary ion transport defects. Proceedings of the Sixth Congress of the International Society for Animal Clinical Biochemistry, Guelph, Canada August 2-6, p 101.

4) Hanna WJB, Jinks RN, Zhang H, Kass L, Renninger GH & Chamberlain SC (1991). Ultrastructure of Limulus photoreceptors in experimental protocols. Society for Neuroscience Abstracts 17:298.

3) Renninger GH & Hanna WJB (1991). Telson photoreceptors in Limulus entrain the visual circadian rhythm of the lateral compound eyes. Society for Neuroscience Abstracts 17:1241.

2) Hanna WJB, Horne JA, Renninger GH, Kaplan E & Barlow RB Jr (1985). The telson of Limulus: an extraocular photoreceptor organ for a circadian clock. Investigative Ophthalmology and Visual Sciences [Suppl] 26:113.

1) Hanna WJB, Pinkhasov E, Renninger GH, Kaplan E & Barlow RB Jr (1985). The telson of Limulus contains photoreceptors that modulate a circadian clock. Biological Bulletin 169:552-553.

Chapters in Books

1) Contributed to the ion channel disease sections of the definitive English-language veterinary pathology text: Jubb, Kennedy, and Palmer's Pathology of Domestic Animals; chapter: "Muscle and Tendon" (van Vleet JF, Valentine BA, chapter editors.). Maxie MG, Slocombe RF, Barker IK, editors. Elsevier; Amsterdam. Published May, 2007.

Case Reports

1) Hanna WJB & Scott WD (1989). An outbreak of placental hydrops in a beefalo herd. Canadian Veterinary Journal 30:597-598.

Last updated August 31, 2016