Molecular & Cellular Imaging Facility

The Molecular and Cellular Imaging Facility is part of the University of Guelph Advanced Analysis Centre (AAC), which provides access (direct or indirect) to highly specialized, state-of-the-art equipment. NMR, Mass Spectrometry, Genomics, and Phytotron are the other facilites within the AAC.

The Advanced Light Microscopy Unit provides microscopy services for internal and external users. Specifically, the facility houses three confocal microscope systems: two Laser Scanning Microscopes (Leica TCS SP5 and Leica TCS SP2) and a Spinning Disk Microscope (Quorum Diskovery) with Super-resolution capability. Additionally, three advanced light microscopes are available for high resolution or high speed and deconvolution imaging (Quorum Riveal Contrast, Leica DM5000B, Zeiss Axiovert 200).

The Electron Microscopy (EM) unit (formerly the Guelph Regional Integrated Imaging Facility [GRIIF]) is open on a cost recovery basis to both Canadian and foreign researchers. The EM Unit consists of two electron microscopes (TEMs), an environmental scanning electron microscope (ESEM) and an array of ancillary preparatory equipment. Each of the TEMs fills a unique niche in the facility; specific details on each of these instruments are provided below.

Confocal Microscopes
Specifications of the Confocal and Light Microcopes in the facility
plant leaf
Electron Microscopes
Specifications of the EMs in the facility
PAO1 biofilm
Support Instruments
Specifications of additional equipment in the facility
Services & Fees
List of available services and fee structure
Leegardiella Protargol
Guidelines & Forms
User guidelines of the facility and safety notes
PAO1 plunge freeze
Manuals & Protocols
Manuals of selected equipment and some general protocols
Cyclotrichium DAPI, Lugol's
Basics & Links
Basic information on microscopy techniques and links to useful websites
Bacillus subtilis
Image Gallery
Example images
Tetrahymena mitochondria
Publications of facility users


Facility logo


University of Guelph
50 Stone Road East
Guelph, Ontario, N1G 2W1