UNIVERSITY OF GUELPH, DEPARTMENT OF PHYSICS, PHOTOBIOPHYSICS LAB 

  lab


News:

 

1. 2015: INTERNATIONAL YEAR OF LIGHT AND LIGHT-BASED TECHNOLOGIES

2. Together with Massimo Olivucci and Hideki Kandori, we organize Retinal Protein Symposium (Chemistry and Applications of Retinal Proteins: From Microbes to Humans (#395)) at Pacifichem 2015.


People in the lab:           

    

DSC_0051.jpgDr. Leonid Brown, Professor and Interim Chair

Department of Physics
University of Guelph
Guelph, Ontario, N1G 2W1
Office MacN 325, Labs MacN 025 and 024
(519) 824-4120 x53777 or x52668, fax: (519) 836-9967, E-mail: lebrown@uoguelph.ca
You can find my personal page at: http://www.uoguelph.ca/~lebrown/

 

 

 

 

 


ward_m.jpgMeaghan Ward, M.Sc., Ph.D. Student (BIG) (co-supervised with V. Ladizhansky), NSERC Scholar, x56439 or x52668

 

 

 

 

 

 

 

 


sanaz.jpgSanaz Emami, M.Sc., Ph.D. Student (BIG) (co-supervised with V. Ladizhansky), Trillium Scholar, x56439 or x52668

 

 

 

 

 

 

 

 

 


https://www.physics.uoguelph.ca/photos/baek_j.jpgJenny (Jihae) Baek, M.Sc. Student (BIG), x53530 or x52668

 

 

 

 

 

 

 


IMG0001 (9)-001.jpgRachel Munro, M.Sc. Student (BIG), NSERC Scholar, x53530 or x52668

 

 

 

 

 

 

 

 

 


Andrew Harris, M.Sc. Student (BIG), x53530 or x52668

 

 

 

 

 

 

 


David Bolton, M.Sc. Student (BIG) (co-supervised with V. Ladizhansky), x56439 or x52668

 

 

 

 

 

 

 


David Park, M.Sc. Student (BIG) (co-supervised with V. Ladizhansky), x52668

 

 

 

 

 

 

 


Milena Ljumovic, Undergraduate Research Assistant, Biochemistry Co-op, x52668

 

 

 

 

 

 

 


Alumni:
Dr. Lichi Shi, M.Sc and Ph.D, Postdoctoral Fellow
lichi

Dr. Arandi G. Bezerra, Jr., Visiting Scientist - now a professor
arandi

Dr. Ying (Cathy) Fan, M.Sc and Ph.D, Research Associate - now a postdoctoral fellow
ying

Mylene Miranda, Ph.D. (BIG) - now a professor
mylene

Donald Fernandes, M.Sc. (BIG)

fernandes_d.jpg

Stephen (Stefko) Waschuk, M.Sc. (BIG) - now a medical writer
stefko
Illya Tolokh, M.Sc. (GWPI) - now an MD/Ph.D. student
ilya
Lin Chen, M.Sc. (BIG)

lin chen

Arthur Rocha, Undergraduate Research Assistant
arthur

Dr. Izuru Kawamura, Visiting Scientist from Yokohama Natl. Univ., now an Assistant Professor

IMG_1003.jpg

Evelyn Lake, Undergraduate Research Assistant - now a graduate student
eve

Dr. Shenlin Wang, Postdoctoral Fellow - now a senior scientist

shenlin

Matthew O'Halloran, M.Sc. Student (BIG) - now an MD student

Eric Poisson, Chair, presents Matthew O'Halloran with the J.B. Reynolds Graduation Medal

Christina Burbadge, Undergraduate Research Assistant - now an MSc student


Collaborations:
Professor Vladimir Ladizhansky (University of Guelph) - Honorary Photobiophysics Lab Member

Professor Kwang-Hwan (Kevin) Jung (Sogang University, Seoul, Korea)
Professor Hideki Kandori (Nagoya Institute of Technology, Japan)

Professor Lars Konermann (Univeristy of Western Ontario)

Professor Hongjun Liang (Colorado School of Mines, USA)
Professor Akimori Wada (Kobe Pharmaceutical University, Japan)

Professor Regis Pomes (SickKids Hospital, Toronto)

Professor Alex Smirnov (North Carolina State Univ., USA)

Professor Alexander Nevzorov (North Carolina State Univ., USA)


Research Topics:

        Photobiophysics, structure/function of membrane proteins, biospectroscopy (visible, infrared, Raman, NMR), retinal-binding proteins, membrane transport.

Research Support:

        The research is (or was) supported by grants from NSERC, CFI/OIT(MRI), PREA, Research Corporation, AFMNet, NRF Korea, and University of Guelph. Our students, postdocs, and visiting scientists are (or were) supported by scholarships and fellowships from NSERC, OGS, CIHR, MITACS, Bruker, Trillium Ontario, and JSPS.

Present Research Activities:

We have been studying a diverse family of photoactive retinal-binding proteins, microbial rhodopsins. We use modern biophysical methods (including time-resolved spectroscopy in the visible, FTIR spectroscopy, NMR, and Raman) in combination with molecular biology. Lately, we also expand into other eukaryotic membrane proteins. Currently, we are working on four major themes.
1) Functional expression of helical eukaryotic membrane proteins for solid-state NMR studies (in collaboration with V. Ladizhansky) - currently includes human aquaporin 1 and A2A receptor.
2) Cyanobacterial rhodopsins. We study a photosensory rhodopsin from cyanobacteria in collaboration with V. Ladizhansky and K.H. Jung (Sogang University).
3) Proteorhodopsin. In collaboration with Vladimir Ladizhansky and K.H. Jung (Sogang University), we explore structure and dynamics of this novel marine proton pump by solid-state NMR and other methods.

4) Novel sodium pumping rhodopsins. In collaboration with Vladimir Ladizhansky, K.H. Jung, and H. Kandori.

Media Links on Our Research:

The Simple Life, Fungus Style (Science Now) snow-header.gif

A primitive energy pathway in fungus (The Scientist and Genome Biology) logo.gif

 

badgef1000.gifFaculty of 1000: The first LR paper (Waschuk et al, PNAS USA, 2005), isotope-labeled LR paper (Fan et al, J. Biomol. NMR, 2011), eubacterial rhodopsins review (Brown, BBA, 2014)    

 

logoAt Guelph: The first LR paper (Waschuk et al, PNAS USA, 2005)

 

Research May Lead to New Drugs for Heart Disease, Other Ailments (Healthcanal, UG Campus News, Guelph Mercury)


                                 PUBLICATIONS (Guelph Photobiophysics people underlined)

97. Choi A.R., Shi L., Brown L.S., Jung K.-H. (2014) Cyanobacterial light-driven proton pump, Gloeobacter rhodopsin: complementarity between rhodopsin-based energy production and photosynthesis. PLoS One, 9: e110643.

96. Johnson P.J., Halpin A., Morizumi T., Brown L.S.Prokhorenko V., Ernst O.P., Miller R.J.D. (2014). The photocycle and ultrafast vibrational dynamics of bacteriorhodopsin in lipid nanodiscs. Phys. Chem. Chem. Phys., 16: 21310-21320.

95. Brown L.S. (2014) Proton-pumping microbial rhodopsins - ubiquitous structurally simple helpers of respiration and photosynthesis. Chapter in The Structural Basis of Biological Energy Generation; Advances in Photosynthesis and Respiration, Springer, 39: 1-20.

94. Good D.B., Wang S.Ward M.E.Struppe J.O., Brown L.S., Lewandowski J.R., Ladizhansky V. (2014) Conformational dynamics of a seven transmembrane helical protein Anabaena Sensory Rhodopsin probed by solid-state NMR. J. Am. Chem. Soc., 136: 2833-2842.

93. Kuang L., Fernandes D.A.O'Halloran M., Zheng W., Jiang Y., Ladizhansky V., Brown L.S., Liang H. (2014) "Frozen" block copolymer nanomembranes with light-driven proton pumping performance, ACS Nano, 8: 537-545.

92. Ward M.E.Wang S., Krishnamurthy S., Hutchins H., Fey M., Brown L.S., Ladizhansky V. (2014) High-resolution paramagnetically enhanced solid-state NMR spectroscopy of membrane proteins at fast magic angle spinning, J. Biomol. NMR, 58: 37-47.

91. Ernst O.P., Lodowski D.T., Elstner M., Hegemann P., Brown L.S., Kandori H. (2014) Microbial and animal rhodopsins: structures, functions and molecular mechanisms. Chem. Rev., 114: 126-163.

90. Brown L.S. (2014) Eubacterial rhodopsins - unique photosensors and diverse ion pumps. Biochim. Biophys. Acta, 1837: 553-561.

89. Wang S.Munro R.Shi L.Kawamura I.Okitsu T., Wada A., Kim S.Y., Jung K.-H., Brown L.S., Ladizhansky V. (2013) Solid-state NMR spectroscopy structure determination of a lipid-embedded heptahelical membrane protein, Nature Methods, 10: 1007-1012.

88. Brown L.S. (2013) A thin line between channels and pumps. Biophys. J., 104: 739-740.

87. Emami S.Fan Y.Munro R.Ladizhansky V., Brown L.S. (2013) Yeast-expressed human membrane protein aquaporin-1 yields excellent resolution of solid-state MAS NMR spectra. J. Biomol. NMR, 55:147-155.

86. Wang S., Shi L., Okitsu T., Wada A., Brown L.S., Ladizhansky V. (2013) Solid-state NMR 13C and 15N resonance assignments of a seven-transmembrane helical protein Anabaena Sensory Rhodopsin. Biomol. NMR Assign., 7: 253-256.

85. Khanal A., Pan Y., Brown L.S., Konermann L. (2012) Pulsed hydrogen/deuterium exchange mass spectrometry for time-resolved membrane protein folding studies. J. Mass Spectrom., 47: 1620-1626.

84. Wang S.Munro R., Kim S.Y., Jung K.-H., Brown L.S., Ladizhansky V. (2012) Paramagnetic relaxation enhancement reveals oligomerization interface of a membrane protein. J. Am. Chem. Soc., 134: 16995-16998.

83. Ito H., Sumii M., Kawanabe A., Fan Y., Furutani Y., Brown L.S., Kandori H. (2012) Comparative FTIR study of a new fungal rhodopsin. J. Phys. Chem. B., 116: 11881-11889.

82. Pan Y., Brown L.S., Konermann L. (2011) Hydrogen exchange mass spectrometry of bacteriorhodopsin reveals light-induced changes in the structural dynamics of a biomolecular machine. J. Am. Chem. Soc., 133: 20237-20244.

81. Ward M.E., Shi L.Lake E.M., Krishnamurthy S., Hutchins H., Brown L.S., Ladizhansky V. (2011) Proton detected solid-state NMR reveals intramembrane polar networks in a seven-helical transmembrane protein proteorhodopsin. J. Am. Chem. Soc., 133: 17434-17443.

80. Fan Y., Solomon P., Oliver R.P., Brown L.S. (2011) Photochemical characterization of a novel fungal rhodopsin from Phaeosphaeria nodorum. Biochim. Biophys. Acta1807: 1457-1466.

79. Wang S.Shi L.Kawamura I.Brown L.S., Ladizhansky V. (2011) Site-specific solid-state NMR detection of hydrogen-deuterium exchange reveals conformational changes in a 7-helical transmembrane protein. Biophys. J., 101: L23-L25.

78. Wang S.Kim S.Y., Jung K.-H., Ladizhansky V., Brown L.S. (2011) A eukaryotic-like interaction of soluble cyanobacterial sensory rhodopsin transducer with DNA. J. Mol. Biol., 411: 449-462.

77. Pan Y., Brown L.S., Konermann L. (2011) Kinetic folding mechanism of an integral membrane protein examined by pulsed oxidative labeling and mass spectrometry. J. Mol. Biol., 410: 146-158.

76. Pan Y., Brown L.S., Konermann L. (2011) Hydrogen/Deuterium exchange mass spectrometry and optical spectroscopy as complementary tools for studying the structure and dynamics of a membrane protein. Int. J. Mass Spectrom., 302: 3-11.

75. Prokhorenko V.I., Halpin A., Johnson P.J.M., Miller R.J.D., Brown L.S. (2011) Coherent control of the isomerization of retinal in bacteriorhodopsin in the high intensity regime. J. Chem. Phys., 134: 085105.

74. Fan Y.Shi L.Ladizhansky V., Brown L.S. (2011) Uniform isotope labeling of a eukaryotic seven-transmembrane helical protein in yeast enables high-resolution solid-state NMR studies in the lipid environment. J. Biomol. NMR, 49: 151-161.

73. Shi L.Kawamura I.Jung K.-H., Brown L.S., Ladizhansky V. (2011) Conformation of a seven-helical transmembrane photosensor in the lipid environment. Angew. Chem. Int. Ed. Engl., 50: 1302-1305.

72. Pan Y., Brown L.S., Konermann L. (2010) Site-directed mutagenesis combined with oxidative methionine labeling for probing structural transitions of a membrane protein by mass spectrometry. J. Am. Soc. Mass Spectrom., 21: 1947-1956.

71. Pan Y., Brown L.S., Konermann L. (2009) Mapping the structure of an integral membrane protein under semi-denaturing conditions by laser-induced oxidative labeling and mass spectrometry. J. Mol. Biol., 394: 968-981.

70. Shi L.Lake E., Ahmed M.A.M., Brown L.S., Ladizhansky V. (2009) Solid-state NMR study of proteorhodopsin in the lipid environment: secondary structure and dynamics. Biochim. Biophys. Acta, 1788: 2563-2574.

69. Shi L., Ahmed M.A.M., Zhang W., Whited G., Brown L.S., Ladizhansky V. (2009) Three-dimensional solid-state NMR study of a seven-helical integral membrane proton pump - structural insights. J. Mol. Biol., 386: 1078-1093.
68. Miranda M.R.M., Choi A.R., Shi L.Bezerra A.G., Jr., Jung K.-H., Brown L.S. (2009) The photocycle and proton translocation pathway in a cyanobacterial ion-pumping rhodopsin. Biophys. J., 96: 1471-1481.
67. Ahmed M.A., Bamm V.V., Shi L., Steiner-Mosonyi M., Dawson J.F., Brown L.S., Harauz G., Ladizhansky V. (2009) Induced secondary structure and polymorphism in an intrinsically disordered structural linker of the CNS: solid-state NMR and FTIR spectroscopy of myelin basic protein bound to actin. Biophys. J., 96: 180-191.
66. Pan Y., Stocks B.B., Brown L.S., Konermann L. (2009) Structural characterization of an integral membrane protein in its natural lipid environment by oxidative methionine labeling and mass spectrometry. Analyt. Chem., 81: 28-35.

65. Shi L., Peng X., Ahmed M.A.M., Edwards D., Brown L.S., Ladizhansky V. (2008) Resolution enhancement by homonuclear J-decoupling: application to three-dimensional solid-state magic angle spinning NMR spectroscopy. J. Biomol. NMR,41: 9-15.
64. Kim S.Y., Waschuk S.A.Brown L.S., Jung K.-H. (2008) Screening and characterization of proteorhodopsin color-tuning mutations in Escherichia coli with endogenous retinal synthesis. Biochim. Biophys. Acta, 1777: 504-513.

63. Prokhorenko V.I., Nagy A.M., Brown L.S., Miller R.J.D. (2007) On the mechanism of weak-field coherent control of retinal isomerization in bacteriorhodopsin. Chem. Phys., 341: 296-309.
62. Magyari K., Simon V., Fan Y.Brown L.S., Varo G. (2007) Absorption kinetics and electrical signals measured on Leptosphaeria rhodopsin. Digest J. Nanomaterials and Biostructures, 2: 265-269. 

61. Fan Y.Shi L.Brown L.S. (2007) Structural basis of diversification of fungal retinal proteins probed by site-directed mutagenesis of Leptosphaeria rhodopsin. FEBS Lett., 581: 2557-2561.
60. Furutani Y., Sumii M., Fan Y.Shi L.Waschuk S.A.Brown L.S., Kandori H. (2006) Conformational coupling between the cytoplasmic carboxylic acid and the retinal in a fungal light-driven proton pump. Biochemistry, 45: 15349-15358.
59. Prokhorenko V.I., Nagy A.M., Brown L.S., Miller R.D. (2006) Experimental coherent control of retinal isomerization in bacteriorhodopsin. Springer Ser. Chem. Phys., 88: 462-464.

58. Prokhorenko V.I., Nagy A.M., Waschuk S.A.Brown L.S., Birge R.R., Miller R.J.D. (2006) Coherent control of the retinal isomerization in bacteriorhodopsin. Science, 313: 1257-1261. Also see the discussion in 317: 453c.
57. Shi L., Yoon S.R., Bezerra A.G., Jr., Jung K.-H., Brown L.S. (2006) Cytoplasmic shuttling of protons in Anabaena sensory rhodopsin: implications for signaling mechanism. J. Mol. Biol., 358: 686-700.

56. Zimanyi L., Saltiel J., Brown L.S., Lanyi J.K. (2006) A priori resolution of the intermediate spectra in the bacteriorhodopsin photocycle: the time evolution of the L spectrum revealed. J. Phys. Chem. A, 110: 2318-2321.
55. Brown L.S., Jung K.-H. (2006) Bacteriorhodopsin-like proteins of eubacteria and fungi: the extent of conservation of the haloarchaeal proton-pumping mechanism. Photochem. Photobiol. Sci., 5: 538-546.
54. Sumii M., Furutani Y., Waschuk S.A.Brown L.S., Kandori H. (2005) Strongly hydrogen-bonded water molecule present near the retinal chromophore of Leptosphaeria rhodopsin, the bacteriorhodopsin-like proton pump from a eukaryote. Biochemistry, 44: 15159-15166.

53. Waschuk S.A.Bezerra A.G., Jr.Shi L.Brown L.S. (2005) Leptosphaeria rhodopsin: bacteriorhodopsin-like proton pump from a eucaryote. Proc. Natl. Acad. Sci. USA, 102: 6879-6883.
52. Garczarek F., Brown L.S., Lanyi J.K., Gerwert K. (2005) Proton binding within a membrane protein by a protonated water cluster. Proc. Natl. Acad. Sci. USA, 102: 3633-3638.
51. Furutani Y., Bezerra A.G., Jr.Waschuk S., Sumii M., Brown L.S., Kandori H. (2004) FTIR spectroscopy of the K photointermediate of Neurospora rhodopsin: structural changes of the retinal, the protein, and the water molecules after photoisomerization. Biochemistry, 43: 9636 -9646.
50. Brown L.S. (2004) Fungal rhodopsins and opsin-related proteins: eukaryotic homologues of bacteriorhodopsin with unknown functions. Photochem. Photobiol. Sci., 3: 555-565.
49. Schobert B., Brown L.S., Lanyi J.K. (2003) Crystallographic structures of the M and N intermediates of bacteriorhodopsin: assembly of a hydrogen-bonded chain of water molecules between asp-96 and the retinal Schiff base. J. Mol. Biol., 330: 553-570.
48. Varo G., Brown L.S., Lakatos M., Lanyi J.K. (2003) Characterization of the photochemical reaction cycle of proteorhodopsin. Biophys. J., 84: 1202-1207.
47. Dioumaev A.K., Brown L.S., Shih J., Spudich E.N., Spudich J.L., Lanyi J.K. (2002) Proton transfers in the photochemical reaction cycle of proteorhodopsin. Biochemistry, 41: 5348-5358. 
46. Brown L.S., Needleman R., Lanyi J.K. (2002) Conformational change of the E-F interhelical loop in the M photointermediate of bacteriorhodopsin. J. Mol. Biol., 317: 471-478. 
45. Dioumaev A.K., Brown L.S., Needleman R., Lanyi J.K. (2001) Coupling of the reisomerization of the retinal, proton uptake, and reprotonation of Asp-96 in the N photointermediate of bacteriorhodopsin. Biochemistry, 40: 11308-11317. 
44. Brown L.S. (2001) Proton transport mechanism of bacteriorhodopsin as revealed  by site-specific mutagenesis and protein sequence variability. Biochemistry (Moscow), 66: 1546-1554. 
43. Brown L.S., Dioumaev A.K., Lanyi J.K., Spudich E.N., Spudich J.L. (2001) Photochemical reaction cycle and proton transfers in Neurospora rhodopsin. J. Biol. Chem., 276: 32495-32505. 
42. Xiao W., Brown L.S., Needleman R., Lanyi J.K., Shin Y.-K. (2000) Light-induced rotation of a transmembrane a-helix in bacteriorhodopsin. J. Mol. Biol., 304: 715-721. 
41. Brown L.S. (2000) Reconciling crystallography and mutagenesis: a synthetic approach to the creation of a comprehensive model for proton pumping by bacteriorhodopsin. Biochim. Biophys. Acta, 1460: 49-59. 
40. Brown L.S., Needleman R., Lanyi J.K. (2000) Origins of deuterium kinetic isotope effects on the proton transfers of the bacteriorhodopsin photocycle. Biochemistry, 39: 938-945. 
39. Dioumaev A.K., Brown L.S., Needleman R., Lanyi J.K. (1999) FTIR spectra of a late intermediate of the bacteriorhodopsin photocycle suggest transient protonation of Asp-212. Biochemistry, 38: 10070-10078. 
38. Varo G., Brown L.S., Needleman R., Lanyi J.K. (1999) Binding of calcium ions to bacteriorhodopsin. Biophys. J., 76: 3219-3226. 
37. Brown L.S., Needleman R., Lanyi J.K. (1999) Functional roles of aspartic acid residues at the cytoplasmic surface of bacteriorhodopsin. Biochemistry, 38: 6855-6861. 
36. Subramaniam S., Lindahl M., Bullough P., Faruqi A.R., Tittor J., Oesterhelt D., Brown L.S., Lanyi J., Henderson R. (1999) Protein conformational changes in the bacteriorhodopsin photocycle. J. Mol. Biol., 287: 145-161. 
35. Brown L.S., Dioumaev A.K., Needleman R., Lanyi J.K. (1998) Connectivity of the retinal Schiff base to Asp-85 and Asp-96 during the bacteriorhodopsin photocycle: the local-access model. Biophys. J., 75: 1455-1465. 
34. Dioumaev A.K., Brown L.S., Needleman R., Lanyi J.K. (1998) Partitioning of free energy gain between the photoisomerized retinal and the protein in bacteriorhodopsin. Biochemistry, 37: 9889-9893. 
33. Brown L.S., Dioumaev A.K., Needleman R., Lanyi J.K. (1998) Local-access model for proton transfer in bacteriorhodopsin. Biochemistry, 37: 3982-3993. 
32. Dioumaev A.K., Richter H.-T., Brown L.S., Tanio M., Tuzi S., Saito H., Kimura Y., Needleman R., Lanyi J.K. (1998) Existence of a proton transfer chain in bacteriorhodopsin: participation of Glu-194 in the release of protons to the extracellular surface. Biochemistry, 37: 2496-2506. 
31. Thorgeirsson T.E., Xiao W., Brown L.S., Needleman R., Lanyi J.K., Shin Y.-K. (1997) Transient channel-opening in bacteriorhodopsin: an EPR study. J. Mol. Biol., 273: 951-957. 
30. Renthal R., Chung Y.-J., Escamilla R., Brown L.S., Lanyi J.K. (1997) Guanidinium restores the chromophore but not rapid proton release in bacteriorhodopsin mutant R82Q. Biophys. J., 73: 2711-2717. 
29. Brown L.S., Kamikubo H., Zimanyi L., Kataoka M., Tokunaga F., Verdegem P., Lugtenburg J., Lanyi J.K. (1997) A local electrostatic change is the cause of the large-scale protein conformation shift in bacteriorhodopsin. Proc. Natl. Acad. Sci. USA, 94: 5040-5044. 
28. Kandori H., Yamazaki Y., Hatanaka M., Needleman R., Brown L.S., Richter H.-T., Lanyi J.K., Maeda A. (1997) Time-resolved Fourier transform infrared study of structural changes in the last steps of the photocycles of Glu-204 and Leu-93 mutants of bacteriorhodopsin. Biochemistry, 36: 5134-5141. 
27. Brown L.S., Needleman R., Lanyi J.K. (1996) Interaction of proton and chloride transfer pathways in recombinant bacteriorhodopsin with chloride transport activity: implications for the chloride translocation mechanism. Biochemistry, 35: 16048-16054. 
26. Chon Y.-S., Sasaki J., Kandori H., Brown L.S., Lanyi J.K., Needleman R., Maeda A. (1996) Hydration of the counterion of the Schiff base in the  chloride transporting mutant of bacteriorhodopsin: FTIR and FT-Raman studies on the effect of anion binding when Asp85 is replaced with a neutral residue. Biochemistry, 35: 14244-14250. 
25. Weidlich O., Schalt B., Friedman N., Sheves M., Lanyi J.K., Brown L.S.,  Siebert F. (1996) Steric interaction between the 9-methyl group of the retinal and tryptophan 182 controls 13-cis to all-trans reisomerization and proton uptake in the bacteriorhodopsin photocycle. Biochemistry, 35: 10807-10814. 
24. Varo G., Brown L.S., Needleman R., Lanyi J.K. (1996) Proton transport by halorhodopsin. Biochemistry, 35: 6604-6611. 
23. Richter H.-T., Brown L.S., Needleman R., Lanyi J.K. (1996) A linkage of the pKa's of Asp-85 and Glu-204 forms part of the reprotonation switch of bacteriorhodopsin. Biochemistry, 35: 4054-4062. 
22. Brown L.S., Lanyi J.K. (1996) Determination of the transiently lowered pKa of the retinal Schiff base during the photocycle of bacteriorhodopsin. Proc. Natl. Acad. Sci. USA, 93: 1731-1734. 
21. Brown L.S., Sasaki J., Kandori H., Maeda A., Needleman R., Lanyi J.K. (1995) Glutamic acid 204 is the terminal proton release group at the extracellular surface of bacteriorhodopsin. J. Biol. Chem., 270: 27122-27126. 
20. Varo G., Brown L.S., Sasaki J., Kandori H., Maeda A., Needleman R., Lanyi J.K. (1995) Light-driven chloride ion transport by halorhodopsin from Natronobacterium pharaonis. 1. The photochemical cycle. Biochemistry, 34: 14490-14499. 
19. Brown L.S., Varo G., Needleman R., Lanyi J.K. (1995) Functional significance of a protein conformation change at the cytoplasmic end of helix F during the bacteriorhodopsin photocycle. Biophys. J., 69:2103-2111. 
18. Brown L.S., Varo G., Hatanaka M., Sasaki J., Kandori H., Maeda A., Friedman N., Sheves M., Needleman R., Lanyi J.K. (1995) The complex extracellular domain regulates the transient deprotonation and reprotonation of the retinal Schiff base during the bacteriorhodopsin photocycle. Biochemistry, 34: 12903-12911. 
17. Sasaki J., Brown L.S., Chon Y.-S., Kandori H., Maeda A., Needleman R., Lanyi J.K. (1995) Conversion of bacteriorhodopsin into a chloride ion pump. Science, 269: 73-75. 
16. Cao Y., Brown L.S., Sasaki J., Maeda A., Needleman R., Lanyi J.K. (1995) Relationship of proton release at the extracellular surface to deprotonation of the Schiff base in the bacteriorhodopsin photocycle. Biophys. J., 68: 1518-1530.
15. Yamazaki Y., Sasaki J., Hatanaka M., Kandori H., Maeda A., Needleman R., Shinada T., Yoshihara K., Brown L.S., Lanyi J.K. (1995) Interaction of tryptophan-182 with the retinal 9-methyl group in the L intermediate of bacteriorhodopsin. Biochemistry, 34: 577-582.
14. Brown L.S., Gat Y., Sheves M., Yamazaki Y., Maeda A., Needleman R., Lanyi J.K. (1994) The retinal Schiff base - counterion complex of bacteriorhodopsin: changed geometry during the photocycle is a cause of proton transfer to aspartate 85. Biochemistry, 33: 12001-12011.
13. Kataoka M., Kamikubo H., Tokunaga F., Brown L.S., Yamazaki Y., Maeda A., Sheves M., Needleman R., Lanyi J.K. (1994) Energy coupling in an ion pump: the reprotonation switch of bacteriorhodopsin. J. Mol. Biol., 243: 621-638.
12. Brown L.S., Yamazaki Y., Maeda A., Sun L., Needleman R., Lanyi J.K. (1994) The proton transfers in the cytoplasmic domain of bacteriorhodopsin are facilitated by a cluster of interacting residues. J. Mol. Biol., 239: 401-414.
11. Cao Y., Brown L.S., Needleman R., Lanyi J.K. (1993) Relationship of proton uptake on the cytoplasmic surface and reisomerization of the retinal in the bacteriorhodopsin photocycle: an attempt to understand the complex kinetics of the pH changes and the N and O intermediates. Biochemistry, 32: 10239-10248.
10. Brown L.S., Zimanyi L., Needleman R., Ottolenghi M., Lanyi J.K. (1993) Photoreaction of the N intermediate of bacteriorhodopsin, and its relationship to the decay kinetics of the M intermediate. Biochemistry, 32: 7679-7685.
9. Brown L.S., Bonet L., Needleman R., Lanyi J.K. (1993) Estimated acid dissociation constants of the Schiff base, Asp-85, and Arg-82 during the bacteriorhodopsin photocycle. Biophys. J., 65: 124-130.
8. Brown L.S., Chamorovsky S.K. (1993) Light adaptation blockage in dehydrated purple membrane does not result from retinal isomerization inhibition. J. Photochem. Photobiol. B., 18: 123-126.

7. Kononenko A.A., Lukashev E.P., Brown L.S., Chamorovsky S.K., Kruming B.A., Yakushev S.A. (1993) Model devices based on bacteriorhodopsin - prototypes of position sensitive photodetectors. Biophysics (Russia), 38: 996-1002.  
6. Brown L.S., Druzhko A.B., Kononenko A.A., Chamorovsky S.K., Shakhbazyan V.Y. (1993) Spectral properties of bacteriorhodopsin analog obtained by reconstitution of bacterioopsin with 4-keto-retinal in vivo. Biol. Membr. (Russia), 10: 140-144.
5. Brown L.S., Druzhko A.B., Chamorovsky S.K. (1992) The light adaptation of bacteriorhodopsin analog with 4-keto-retinal. Biophysics (Russia), 37: 59-65.  
4. Brown L.S., Druzhko A.B., Lukashev E.P., Chamorovsky S.K. (1991) Investigation of the photochemical cycle of a bacteriorhodopsin analog containing 4-keto-retinal residue. Biol. Membr. (USSR), 8: 460-467. 
3. Brown L.S., Semin B.K., Ivanov I.I., Kononenko A.A., Chamorovsky S.K., Churin A.A. (1991) Tetracaine induces the formation of the spectral form of bacteriorhodopsin BR-470 in purple membranes of halobacteria. Biochemistry (USSR), 56: 241-249. 
2. Brown L.S., Kononenko A.A., Protasova T.B., Fedorovich I.B., Chamorovsky S.K. (1990) Effects of the ultraviolet radiation on bacteriorhodopsin. Radiobiol. (USSR), 30: 506-511. 
1. Chamorovsky S.K., Sabo J., Brown L.S. The automated pulse laser spectrophotometer to investigate processes of a photoinduced charge transfer in photosynthesis (1989) Biol. Sci. (USSR), 6: 98-100.