C. Protocols

Protocols for Sample Handling(pdf)
1.
An Official OMVFSP Test requires the following:
a.
All official serological samples for any MV testing must be collected by, or their collection supervised by one of the following:
i.
A veterinarian licensed in the province in which the flock resides.
ii.
A registered veterinary technician (RVT) or equivalent employed by and directly supervised by a veterinarian licensed in the province in which the flock resides.
iii.
Not the owner or manager, spouse, immediate relative or employee of the owner or manager of the flock, even if he or she is a licensed veterinarian or RVT may supervise the collection of the samples, although they may participate in the sample collection as long as supervised by a veterinarian licensed in the province in which the flock resides.
b.
That the serological test used be one of those listed in Section B.10.a.i.
2.
Sample collection and record keeping:
a.
Whole Flock program: all sheep and goats ≥ 180 days of age that reside at the flock premise on the date of the test must be sampled.
i.
Sheep that are isolated from the flock and will be sent for slaughter within 30 days of the test date do not need to be sampled.
 
(1)
E.g. market lambs ≥ 180 days of age.
(2)
E.g. adults marked for culling.
b.
Monitored or Whole Flock – “A” Status program: a random sample selected from all sheep and goats ≥ 365 days of age that reside at the flock premise on the date of the test must be sampled.
c.
A minimum of 5 mL of whole clotted blood or 1 mL serum or plasma to be collected by venipuncture per sheep tested. 
i.
Each tube must be clearly and uniquely identified.
ii.
Usually laboratories will assess an additional cost for serum separation.
d.
Ensure correct identification of the sheep and lambs and record this ID with the corresponding correct tube number on an official OMVFSP form (Example given in APPENDIX 6).  These forms are available from OSMA and will be provided to producers enrolled on the OMVFSP.
e.
Complete the OMVFSP sample submission form entirely and properly.
i.
The form that is currently available from OSMA is specifically for use in the AHL, University of Guelph.  If you wish to use a different laboratory, they must provide a suitable diagnostic test (as listed in Section B.10.i.).  Contact OSMA for further instructions.
f.
Initiate rapid transport of the coagulated blood or serum and all required documents to the official laboratory within 24 hrs of sampling.  Ensure that the blood will remain chilled but not frozen during transport.  If serum is separated prior to shipping, it may be frozen.
g.
Whole Flock and Monitored-Low Risk status flocks.  All sheep NOT sampled that reside at that premise on the date of the Official Test must be identified as set out in this protocol, and that ID recorded on an official OMVFSP form obtained from OSMA.
i.
Sheep not sampled include lambs < 180 days of age and animals ≥ 180 days of age that will leave the flock in the next 30 days.
ii.
A Microsoft Excel spreadsheet OMVFSP_AHL_Inventory_Form is available from OSMA that will allow for electronic entry and tracking of sheep tested, as well as sheep not tested that reside on the premise on the Official Test date.  OSMA maintains each flock's up-to-date inventories and results and so the worksheet must be obtained from the Project Coordinator. Worksheets included in this spreadsheet:
 
(1)
Animals Tested list.  To facilitate proper tracking of animals for both OSMA and your veterinarian, enter information on all animals tested on the flock test.  It is formatted so that data can be copied and pasted into AHL forms for easier submission.
 
(2)
Animals Not Tested list.  All sheep that resided in the flock on the day of the test and are not tested must be listed on this sheet.  This includes animals to be culled or marketed in next 30 days from the date of the Official Test, and lambs < 180 days of age.  Any animals not on the subsequent list that are not a natural increase (i.e. offspring of ewes in the flock), or do not have the correct testing and transfer forms, will be assumed to be illegal entries and may jeopardize the status of the flock
 
(3)
Animals Left the Flock list.  All sheep that were on a previous inventory but have left the flock for any reason including death, should be recorded here so that they can be permanently deleted from the inventory maintained by OSMA.  
3.
All official submission forms (APPENDIX 6) must be signed by the supervising licensed veterinarian verifying that
a.
The samples were obtained from the animals identified on the form, that
b.
All sheep and lambs were correctly identified and those ID’s recorded, and that
c.
The protocols for the OMVFSP have been followed.

4.

The samples must be sent to a laboratory that offers the diagnostic tests identified in Section B.10.a.i, and is recognized by the Ontario Sheep Marketing Agency in the OMVFSP, i.e.

 
a.
The Animal Health Laboratory
  Veterinary Services Division                                  
University of Guelph                                             
50 Stone Road                                                     
Guelph, Ontario                                                     
CANADA   N1G 2W1 
Email: ahlinfo@uoguelph.ca

Telephone: (519) 824-4120 ext 54530
Fax: (519) 827-0961
Web: http://www.guelphlabservices.com/AHL/
Client Services Veterinarian: Dr. Jim Fairles
Telephone:  (519) 824-4120 ext 54611
i.
This veterinary diagnostic laboratory offers the HYPHEN Elitest for MV testing.

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