Lv R, Zheng L, Zhu Z,Pan L, Huang J, Hsiang T. 2011. First report of stem blight of Eleocharis dulcis caused by Phoma bellidis in China. Plant Disease. Journal Website Reprint
Eleocharis dulcis is a perennial herbaceous plant in the family Cyperaceae that is native to China and India, where it grows well in moist to wet soils. Corms from the plant are commonly used as a fruit or a vegetable. From August 2009 to December 2010, symptoms were observed on E. dulcis stems in Tuanfeng County, Hubei, China, with the crop area affected estimated to be over 1300 ha per year. Corm yield was reduced by 20% on average with up to 60% yield losses in some fields. Lesions were initially small, red-brown, and oval or circular, and these enlarged to produce apical necrosis, extending until the stems withered usually within two months. To obtain isolates, diseased tissue was disinfested for 1 min in 0.1% mercuric chloride solution, rinsed with sterilized water, and plated on potato dextrose agar (PDA). Isolates with similar morphological characteristics were consistently recovered. Three isolates, CTF-3, CTF-10 and CTF-11, were used to further evaluate characteristics of the pathogen. After 7 days, white colonies were 76 to 80 mm across on oatmeal agar (OA), with sparse aerial hyphae and slight salmon color in conidial masses. Pycnidia produced on OA were globose to subglobose, usually with one slightly ostiolar papilla, olivaceous to olivaceous black, and 93 to 245 μm in diameter. Conidia were hyaline, unicellular, ellipsoidal, mostly with two polar guttules, and 3.6 to 6.2 x 2.0 to 3.3 μm. Chlamydospores were absent. Growth of the isolates on malt extract agar (MEA) was slower than on OA, and the colony diameters at 7 days were 60 to 65 mm. Reactions with 1M NaOH were both positive on OA and MEA where the cultures initially changed to yellow green and gradually turned to red. The pathogen was identified as Phoma bellidis Neerg. based on descriptions in Boerema et al. (2). Pathogenicity tests were performed with the three isolates in the laboratory by spraying conidial suspensions (1x106 conidia/ml) containing 0.1% Tween-20 until runoff (30 ml per plant) onto stem surfaces of 50-day-old 60-cm tall plants. For each isolate, there were 50 stems from five replicate plants that had multiple stems per plant. Control plants were treated with sterilized water containing 0.1% Tween-20 only. Plants were incubated with a 16-h photoperiod at 28 C and 90% relative humidity in an artificial climate chamber. Five days after inoculation, typical red-brown spots were observed on all inoculated stems but no symptoms were seen on water-treated control plants. Koch's postulates were fulfilled by re-isolation of P. bellidis from diseased stems. The pathogenicity tests were repeated twice more with the same results. Phoma bellidis has only been reported previously on Bellis spp. from England, Denmark, Italy, Netherlands and Switzerland (1, 2). Furthermore, there are only a few fungal diseases known to be associated with E. dulcis, and none so far that involve species of Phoma (3, 4). To our knowledge, this is the first report of P. bellidis infecting E. dulcis worldwide.

References: (1) M. M. Aveskamp et al. Stud. Mycol. 65: 27, 2010. (2) G. H. Boerema et al. Phoma identification manual: differentiation of specific and infra-specific taxa in culture. CABI Publishing, Wallingford, UK, 2004. (3) P. L. Lentz. Am. Midl. Nat. 67: 184, 1962. (4) L. Pan et al. J. Changjiang Vegetables (In Chinese) 14: 10, 2010.