Genomics Services
Agilent BioAnalyzer 2100
Please note, Bioanalyzer Service has been replaced with the TapeStation Service.
Equipment Used: Agilent TapeStation 4150
Agilent TapeStation 4150 Service
The TapeStation system allows for automated electrophoresis of most DNA and RNA samples. Typical applications include quality control (QC) of NGS libraries and RNA extractions (ie. RIN). This method excels in the determination of fragment length distribution of nucleic acid samples, but is not typically relied on for accurate quantification. The TapeStation can be used in place of the BioAnalyzer for most applications, but cannot currently be used for small/micro RNA analysis.
Service includes Standard and High Sensitivity assays for RNA, DNA >1kb, and DNA >5kb, as well as a Standard gDNA assay.
Request Form: AAC TapeStation Request Form
Equipment Used: Agilent TapeStation 4150
DNA Sequencing
Sample submission considerations.
The facility provides a number of standard sequencing primers for common plasmid vectors.
Custom primers should be provided at a concentration of 5 uM (=5 pmol/uL).
Request Forms:
Individual Sample Submissions
Sanger Sequencing Request - Use this form to submit DNA templates (ie. PCR amplicons, Plasmids, etc.) for sequencing
DIY Sequencing Request - Use this for to submit completed flourescent Sanger products (ie. BigDye reactions) for sequencing
For full and half-plate submissions please contact the facility
Equipment Used: Applied Biosystems 3730 DNA analyzer
Fragment Analysis
Gene Expression Services
- Real Time PCR
- Digital Droplet PCR
Request Forms:
Digital Droplet PCR Request
Real-Time PCR Registration
Real-Time PCR Request
Equipment Used:
BioRad Qx200 Droplet Digital PCR (ddPCR) System
StepOnePlusTM Real-Time PCR System
Next Generation Sequencing using MiSeq desktop sequencer
- Metagenomics
- Small genome sequencing
- Amplicon sequencing
- Targeted gene sequencing
Equipment Used: Illumina MiSeq Sequencer
Nucleic Acid Fluorometry (Qubit)
Fluorometric concentration assays are generally more accurate than spectrophotometric/UV-Vis absorbance (ie. Nandrop) measurements because they are less affected by common contaminants (ie. Proteins, RNA, or DNA).
This method is recommended NGS library assessments and for any application that requires higher accuracy in concentration estimates than can be acheived with Nanodrop.
Request Form:
Qubit Request
Equipment Used: Qubit Flex Fluorometer, Qubit® 2.0 Fluorometer