Mass Spectrometry Buffers

This table shows the MAXIMUM concentration of surfactants, buffers and salts permissible in Maldi and Electrospray ionization techniques.  N/A means the technique is not compatible with ANY amount of indicated compound.  (Modified from "The Expanding Role of Mass Spectrometry in Biotechnology" by Gary Siuzdak, 2003, pages 84-85)

Surfactants, Buffers, Salts For MALDI (mM)
(currently unavailable)		 For ESI (mM)
TRIS 100 N/A
HEPES 100 N/A
BICINE 50 N/A
Urea 500 N/A
Guanidine, HCl 250 N/A
Dithiothreitol 500 N/A
Glycerol 130 N/A
n-Octyl-b-glucopyranodise 304 3.4
n-Octyl-sucrose N/A 2.1
n-Dodecyl sucrose N/A 1.9
n-Dodecyl maltoside N/A 2.0
Octyl thioglucoside N/A 3.2
n-Hexyl gluocside N/A 3.8
n-Dodecyl glucoside N/A 2.9
PEG1000 N/A 0.5
PEG2000 0.5 N/A
Triton X-100 1.6 less than 1.6
NP-40 1.7 N/A
Zwittergent, 3-16 2.6 N/A
Tween 20 N/A 0.81
Thesit N/A less than 1.7
SDS 0.35 less than 4.4
LDAO 4.4 less than 4.4
CTAB N/A less than 3.5
CHAPS 0.16 1.6
Sodium Cholate N/A 2.3
Sodium Taurocholate N/A less than 1.9
Sodium Azide 15 3.1
NH4CHO3 50 N/A
NaCl 50 N/A
Sodium Acetate 50 N/A
NaHPO4 10 10
TFA N/A 4.4