Final Examination for the Degree of MSc - Yuhan Zeng
Date and Time
Food Science lecture room 128
Final Examination for the Degree of MSc
Dr. Art Hill, Chair
Dr. Yoshi Mine, Advisor
Dr. Rong Cao, Advisory Committee Member
Dr. Keith Warriner, Department Examiner
TITLE: ANTI-INFLAMMATORY ACTIVITY OF HEAT-KILLED PROBIOTICS, LACTOBACILLUS
PENTOSUS S-PT84 IN A C57BL/6J MOUSE MODLE WITH LPSINDUCED LOW-GRADE
ABSTRACT: This study is aimed to identify the anti-inflammatory activity and mechanism of the heat-killed probiotics, Lactobacillus pentosus S-PT84, in a mouse model with lipopolysaccharide (LPS)-induced systemic low-grade chronic inflammation. The persistent increase of intestinal permeability contributes to the development of intestinal inflammation and systemic inflammation. Systemic low-grade chronic inflammation is tightly related to insulin resistance and metabolic diseases. Pre-treatment of Lactobacillus pentosus S-PT84 decreased the production of proinflammatory mediators, TNF-α and MCP-1, and stimulated the production of anti-inflammatory mediator, adiponectin in plasma by increasing the protein expression of peroxisome proliferatoractivated receptor gamma (PPAR-γ) in the white adipose tissues. Lactobacillus pentosus S-PT84 maintained the structural integrity of intestines and homeostasis of intestinal permeability via modulating the expression of tight junction proteins. The intact integrity of mucosal barrier reduced the entrance and content of inflammatory stimulants in the circulating system.
Moreover, Lactobacillus pentosus S-PT84 exerted the protective effects on preventing the insulin resistance by increasing the insulin sensitivity, glucose tolerance, and protein expression of insulin receptor subset-1 (IRS-1).
This study provides an evidence that Lactobacillus pentosus S-PT84 possesses anti-inflammatory properties and exerts beneficial functions to prevent systemic low-grade chronic inflammation and reduces the risk of developing insulin resistance and associated metabolic diseases.