Analytical Biochemistry (BIOC*3570)

Code and section: BIOC*3570*01

Term: Summer 2017

Instructor: D. Josephy

Details

BIOC*3570 Summer 2017 Lecture Schedule (subject to modification):

Class

Date

Topic

Readings

1

2017-05-11

Introduction. 

16-20

2

2017-05-16

Protein quantitation

306-311

3

2017-05-18

Protein purification principles

"

4

2017-05-23

Chromatography principles; diffusion

433-437

5

2017-05-25

Ion-exchange chromatography

459-461

6

2017-05-30

Gel-filtration chromatography

462-465

7

2017-06-01

Affinity chromatography

465-470

8

2017-06-06

Electrophoresis: theory

399-411

9

2017-06-08

Electrophoresis: techniques; detection of proteins

417-418

10

2017-06-13

Antibodies

263-279

11

2017-06-15

Immuno- ("western") blotting

419-422

12

2017-06-20

Electrophoresis: 2D gels; proteomics

411-415; 341-345

13

2017-06-22

Class test #1

 

14

2017-06-27

Centrifugation: theory

73-95

15

2017-06-29

Centrifugation: techniques

"

16

2017-07-04

Analytical ultracentrifugation

95-99

17

2017-07-06

UV-visible spectroscopy: theory

477-487

18

2017-07-11

UV-visible spectroscopy: instrumentation and applications

487-493

19

2017-07-13

Fluorescence spectroscopy: theory

493-501

20

2017-07-18

Class test #2

 

21

2017-07-20

Fluorescence spectroscopy: instrumentation and applications

493-501

22

2017-07-25

Mass spectrometry principles; term paper due

chapter 9

23

2017-07-27

Isotope distributions; molecular weights of macromolecules

"

24

2017-08-01

Protein mass spectrometry; proteomics

"

 

 

 

 

 

 

Final exam.

 

Syllabus

AttachmentSize
PDF icon BIOC*3570, Course Outline, Summer 2017429.21 KB