Research Lab Report 1: Research Methods, Context and Question

This report will contain the information listed below. The page limits for the text are exclusive of figures, tables and references.

Title

A concise statement of your research topic

Article:

The complete reference for your article, in Molecular Microbiology format.

Table of Research Methods :

Turn the list of methods used by your researchers (already submitted to your TA) into a Table. Indicate which methods are standard to microbiology (see list in Lab 3). Cite references that provide explanations for at least three of the listed methods (assigned by your TA). Indicate which method will be described in this report. This table should be approximately 1 page long and follow the format below.

Table of Research Methods
# Method Reference
1N/A means Not Applicable. This method is standard to microbiology. 2This method is particularly critical to the reported research and is therefore described below.
1 Inoculation of petri plates N/A1
2 Bacterial cell culture N/A
3 Cell Disruption (French Pressure Cell) (1)
4 Cell Fractionation by Differential Centrifugation (2)
5 Enzyme Purification by Ni(NTA) Affinity Chromatography (3)2
6 Protein Assay (4)
7 A Specific Enzyme Assay (5)2

For example, in the table above (1) might refer to appropriate pages in Gerhardt's Methods for General and Molecular Bacteriology, (2) might refer to Gerhardt's book again, or to Freifelder's Physical Biochemistry: Applications to Biochemistry and Molecular Biology, (3) might refer to a document provided online by Qiagen Inc., (4) might refer to a journal article that describes a particular protein assay and (5) might refer to a relevant volume of Methods in Enzymology or a journal article. If a method is described fully in the article you are analyzing, your article can be used as the citation for that method. Please do not cite undergraduate lab manuals (they are not published) or online lab protocols (they have not been validated by peer review).

Explanation of a Research Method (approximately 2 pages of text)

Explain the method that you selected during Lab 3 by answering these questions:

  • How is the method performed?
  • What is accomplished in each step, and how does each step work?
  • What data are obtained?
  • How are the data interpreted?
  • What information is gained by using this method?

Usually diagrams help to explain research methods. You are encouraged to include diagrams in your report (preferably your own diagrams - they are not included in your page limit!).

In writing this explanation, imagine that you are answering the questions above for a student colleague who is not familiar with the selected method. Examples of good explanations can be found in some textbooks and in the reference books cited in Lab 3. Also, think about the best lab manuals provided during your undergraduate studies. Your goal will be to write such an explanation of the most important/novel method used by your authors, in your own words.

Research Context and Question(s) (approximately 5.5 pages of text)

Summarize the intellectual and human contexts of the research reported in your article, including answers to all the questions considered during labs 4 and 5 (2 and 3 pages of text, respectively). Using up to 1/2 page of text, state the questions asked/hypotheses tested by the authors of your article.

Reference List

List the references cited in your report, using the citation format of Molecular Microbiology. Marks will be deducted if the reference citation format is incorrect. Remember that this is a reference list, not a bibliography — every item in your reference list must be cited in the text of your Report. Check the Lab Orientation page and the Help page to make sure that you cite references correctly and avoid plagiarism (a serious academic offense).

Report Format

The report must be typed, double spaced and with 2 cm margins, using a 12 point font. It should include a title page (title, article citation and your name), and approximately 7.5 additional pages of text, excluding tables, figures and reference list. The pages must be numbered, with the title page as page 1.

Deadline

Completed Lab Reports must be delivered, in the format specified above, as a hard copy to room SCIE4251 and as an electronic copy to the Courselink Dropbox, both before 4 pm on the dates specified on Courselink. This deadline will be extended only if you provide appropriate documentation (otherwise a grade of zero will be assigned). Computer problems are not an acceptable basis for deferral, so take care to back up your work!

Grading

MICR*3260 Microbial Adaptation and Development, Report 1 Grading

Below is a table illustrating how your Research Lab Report 1: Research Methods, Context and Question will be graded.

Report 1 Grading Components and Marks
Component Criteria Marks
Total Grade(20)
List of Methods
  • Was the submitted list complete?
  • Was an appropriate Method selected for discussion in Report 1?
  • Were the references associated with the final list in Report 1 appropriate and complete?
3 of 20 marks
Explanation of Methods
  • How is the method performed?
  • What data are obtained?
  • How are the data interpreted?
  • What information is gained?
4 of 20 marks
Human Context
  • Who did the work, where and with what support?
  • Where was the work published?
  • Why did the authors do this research?
4 of 20 marks
Report on Research Publications
  • Are PubMed links provided for all authors?
  • Is each search appropriate?
1 of 20 marks
Intellectual Context
  • What was known at the time?
  • Who else was interested?
  • Are specific publications discussed and cited to illustrate these marks (including some not cited in the article under analysis)?
5 of 20 marks
Hypothesis / Question What question did the researchers ask? / What hypothesis did they test? 1 of 20 marks
Editorial Quality Spelling, grammar, style, reference format and length limits. 2 of 20 marks