Fluorescent Nucleobase Surrogate and Aptasensor

A chemical structure of the fluorescent nucleobase surrogate containing a donor and an acceptor moiety.


  • Aptamer that changes fluorescence upon binding to target.
  • Cyanine-indole-quinolinium (4QI) dye joined to DNA aptamers.
  • Fluorescent detection of aptamer-target binding.
  • Rapid signal development (instantly upon binding).
  • 90nm Stokes shift makes it particularly usable in difficult matrices, or minimally prepared samples (i.e. blood, raw water, etc.)
  • Proof of concept stage attained (tests have been performed against biomolecules in complex matrices and small molecules in solution.)
  • Derivative and new variant technologies are currently under development.

Advantages and Applications

  • Useful for: biomedical diagnostics, research, and quantification as well as environmental imaging and diagnostics.
  • Single strand procedure - no need to optimize complementary strand equilibria.
  • Self-attenuating, eliminates additional steps in measurement.
  • Dye responds only during target binding with negligible background.


Utility patent granted in the United States (US 11,215,619) and pending in Canada (CA 3063152)

Seeking licensee(s).


Michael Fowler, michaelf@uoguelph.ca, 519-824-4120 x53808