Department of Molecular and Cellular Biology Faculty . Dr. John P. Phillips

Email: jphillip@uoguelph.ca

 

Education

B.Sc. Emporia
M.Sc. Utah State
Ph.D. Utah State

Research

Genetic and molecular studies of the role of oxygen radical metabolism in disease and aging. Identification and molecular characterization of genes specifying oxygen radical defense functions. Generation of mutant and transgenic models of disease and aging in mice and Drosophila. Application of gene transfer biotechnology to overcome environmental and nutritional problems in animal agriculture. Development of the Enviropig™.

Selected Publications

Anderson, P.R., Kirby, K., Hilliker, A.J. and Phillips, J.P. (2005) RNAi-mediated suppression of the mitochondrial iron chaperone, frataxin, in Drosophila. Human Molecular Genetics, 14, 3397-405. Available online at http://hmg.oxfordjournals.org/cgi/content/full/14/22/3397

Woodruff, R. C., Phillips, J. P. and A. J. Hilliker (2004) Increased Spontaneous DNA Damage in Cu/Zn Superoxide Dismutase (SOD1) Genome 47: 1029-1035.

Missirlis F., Hu, J., Kirby, K., Hilliker, A. J., Rouault, T. A. and J. P. Phillips (2003) Compartment-Specific Protection of Iron-Sulfur Proteins by Superoxide Dismutase. J Biol Chem 248: 47365-47369.

Ajakaiye, A., M. Z. Fan, T. Archbold, R.R. Hacker, C.W. Forsberg and J.P. Phillips. (2003). Determination of true digestive utilization of phosphorus and the endogenous outputs associated with soybean meal for growing pigs. J. Anim. Sci. 81: 2766-2775.

Missirlis, F., Rahlfs, S., Dimopoulos, N., Bauer. H., Becker, K., Hilliker, A., Phillips, J. P., and Jäckle, H. (2003) A putative glutathione peroxidase of Drosophila encodes a thioredoxin peroxidase that provides resistance against oxidative stress but fails to complement a lack of catalase activity. Biol Chem 384: 463-472.

Forsberg, C. W., Phillips, J. P., Golovan, S. P., Fan, M. Z., Meidinger, R. G., Ajakaiye, A., Hilborn, D., Hacker, R. R. (2003) The EnviropigTM physiology, performance, and contribution to nutrient management, advances in a regulated environment: The leading edge of change in the pork industry. J. Anim. Sci. 81(E. Suppl. 2):E68-E77.

Kirby, K., Hu, J., Hilliker, A. J., and Phillips, J. P. (2002). RNA interference-mediated silencing of Sod2 in Drosophila leads to early adult-onset mortality and elevated endogenous oxidative stress. Proc Natl Acad Sci USA. 99: 16162-16167.

Missirlis, F., Ulschmid, J. K., Hirosawa-Takamori, M., Grönke, S., U., S., Becker, K., Phillips, J. P., and Jäckle, H. (2002). Mitochondrial and cytoplasmic thioredoxin reductase variants encoded by a single Drosophila gene are both essential for viability. J Biol Chem 277: 11521-11526.

Cottrill, M. A., Golovan, S. P., Phillips, J. P., and Forsberg, C. W. (2002). Inositol phosphatase activity of the Escherichia coli agp-encoded acid glucose-1-phosphatase. Can J Microbiol 48, 801-809.

Missirlis, F., Phillips, J. P., and Jäckle, H. (2001). Cooperative action of antioxidant defense systems in Drosophila, Current Biology 11: 1-20.

Golovan, S. P., Meidinger, R. G., Ajakaiye, A., Cottrill, M., Wiederkehr, M. Z., Barney, D., Plante, C., Pollard, J., Fan, M. Z., Hayes, M. A., Laursen, J., Hjorth, P. J., Hacker, R. R., Phillips, J. P. and Forsberg, C. W. (2001) Pigs expressing salivary phytase produce low phosphorus manure through complete digestion of dietary phytate. Nature Biotech 19: 741-745.

Golovan, S. P., Hayes, M. A., Phillips, J. P. and Forsberg, C. W. (2001) Transgenic mice expressing bacterial phytase as a model for phosphorus pollution control. Nature Biotech.19: 429-433.

Jia, Z., Cottrill, M., Pal, G. P., Lee, D., Sung, M., Forsberg, C. W., and Phillips, J. P. (2001). Purification, crystallization and preliminary X-ray analysis of the Escherichia coli glucose-1-phosphatase, Acta Cryst. D57: 314-316.

Phillips, J.P., Parkes, T.L. and Hilliker, A.J. (2000) Targeted Neuronal Gene Expression and Longevity in Drosophila. Exp. Gerontol. 35: 1157-1164.

Wang, J.W., Humphreys, J.M., Phillips, J.P., Hilliker, A.J. and Wu, C.-F. (2000) A Novel Leg-Shaking Drosophila Mutant Defective in a Voltage-gated K+ Current and Hypersensitive to Reactive Oxygen Species. J. Neurosci. 20: 5958-5964.

Draper, H. H., Philbrick, D. P., Agarwal, S. and Phillips, J. P. (2000) Avid uptake of linoleic acid and vitamin E by Drosophila melanogaster. Nutr. Res. 20:113-120.