Required Forms:
OAHN Swine Small Scale Herd Postmortem Project-Survey [1]
OAHN Swine Small Scale Herd Postmortem Project - Submission form [2]
Sudden Death in Nursing Piglets tissue sampling list [3]
TISSUE SAMPLING LIST-collect the following samples from EACH pig
|
Fresh tissues: 1 sample per Whirl-Pak bag, per pig – label with stickers provided. Formalin-fixed tissues: 1 sample jar per pig. |
| Tissue | Fresh (include lesions) | In Formalin (include lesions) |
| Tonsil | X | X |
| Thyroid gland | X | |
| Trachea | X | |
| Thymus | X | |
| Lung | X3 | X |
| Heart | X | |
| Liver | X | X |
| Spleen | X2 | X |
| Kidney | X | X |
| Adrenal gland | X | |
| Pancreas | X | |
| Mesenteric lymph node | X | X |
| Gastrohepatic lymph node | X | X |
| Stomach | X | |
| Duodenum | X | |
| Jejunum | X | |
| Ileum | X | |
| Spiral colon | X | |
| Diaphragm | X | |
| Skeletal muscle | X | |
| Brain (or head) | X half, sagitally sectioned (OR entire head-fresh) | X half, sagitally sectioned (OR entire head-fresh) |
Sudden Death in Nursing Piglets
Fresh tissues: Use a separate Whirl-Pak bag for each sample – use provided stickers and marker to label the bag with the tissue name and pig designation (pig A, B, or C).
Formalin-fixed tissues: Use 1 sample jar per pig and label with pig designation (pig A, B, or C). For solid organs, samples should be approximately 1-2 cm2. For intestine, take approximately 3 cm long segments and partially open longitudinally with scissors (to allow formalin to contact the mucosal surface).
Test list (for AHL use):
Virology: PRRSV PCR prtqn, influenza A virus PCR inflpcr: lung (pooled)
Bacterial culture bsetup, cults: lung, spleen (pooled by tissue)
Histopathology hist
Freeze and hold: tonsil /spleen, lung, kidney/ liver, lymph nodes, brain.