Required Forms:
OAHN Swine Small Scale Herd Postmortem Project-Survey [1]
OAHN Swine Small Scale Herd Postmortem Project - Submission form [2]
Sudden Death in Nursery (weaner) Pigs tissue sampling list [3]
TISSUE SAMPLING LIST-collect the following samples from EACH pig
|
Fresh tissues: 1 sample per Whirl-Pak bag, per pig – label with stickers provided. Formalin-fixed tissues: 1 sample jar per pig. |
| Tissue | Fresh (include lesions) | In Formalin (include lesions) |
|
Tonsil |
X |
X |
|
Thyroid gland |
|
X |
|
Trachea |
|
X |
|
Thymus |
|
X |
|
Lung |
X 3 |
X |
|
Heart |
|
X |
|
Liver |
X |
X |
|
Spleen |
X 2 |
X |
|
Kidney |
X |
X |
|
Adrenal gland |
|
X |
|
Pancreas |
|
X |
|
Mesenteric lymph node |
X |
X |
|
Gastrohepatic lymph node |
X |
X |
|
Stomach |
|
X |
|
Duodenum |
|
X |
|
Jejunum |
|
X |
|
Ileum |
|
X |
|
Spiral colon |
|
X |
|
Diaphragm |
|
X |
|
Skeletal muscle |
|
X |
|
Brain (or head) |
X half, sagitally sectioned (OR entire head, fresh) |
X half sagitally sectioned (OR entire head, fresh) |
Sudden Death in Nursery (Weaner) Pigs (1-3 animals <40 kg)
Fresh tissues: Use a separate Whirl-Pak bag for each sample – use stickers and marker provided to label bags with the tissue name and pig designation (pig A, B, or C).
Formalin-fixed tissues: Use 1 sample jar per pig and label with pig designation (pig A, B, or C). For solid organs, samples should be approximately 1-2 cm2. For intestine, take approx. 3 cm long segments and partially open longitudinally with scissors (to allow formalin to contact the mucosal surface).
Test list (for AHL use):
Virology: PRRSV PCR prtqn, influenza A virus PCR inflpcr: lung
Bacterial culture bsetup, cults: lung, spleen (pool by tissue)
Histopathology hist
Freeze and hold: tonsil / spleen, lung, kidney/ liver, lymph nodes, brain.