Required Forms:
OAHN Swine Small Scale Herd Postmortem Project-Survey [1]
OAHN Swine Small Scale Herd Postmortem Project - Submission form [2]
Respiratory Disease in Nursery (weaner) Pigs tissue sampling list [3]
TISSUE SAMPLING LIST-collect the following samples from EACH pig
Fresh tissues: 1 sample per Whirl-Pak bag, per pig – label with stickers provided. Formalin-fixed tissues: 1 sample jar per pig. |
Tissue | Fresh (include lesions) | In Formalin (include lesions) |
Tonsil |
X |
X |
Thyroid gland |
|
X |
Trachea |
|
X |
Thymus |
|
X |
Lung |
X 4 |
X |
Heart |
|
X |
Liver |
X |
X |
Spleen |
X |
X |
Kidney |
X |
X |
Adrenal gland |
|
X |
Pancreas |
|
X |
Mesenteric lymph node |
X |
X |
Gastrohepatic lymph node |
X |
X |
Stomach |
|
X |
Duodenum |
|
X |
Jejunum |
|
X |
Ileum |
|
X |
Spiral colon |
|
X |
Diaphragm |
|
X |
Skeletal muscle |
|
X |
Brain (or head) |
X half, sagitally sectioned (OR entire head, fresh) |
X half, sagitally sectioned (OR entire head, fresh) |
Respiratory Disease in Nursery (Weaner) Pigs (<20 kg, 1-3 animals)
Fresh tissues: Use a separate Whirl-Pak bag for each sample – use provided stickers and marker to label the bag with the tissue name and pig designation (pig A, B, or C).
Formalin-fixed tissues: Use 1 sample jar per pig and label with pig designation (pig A, B, or C). For solid organs, samples should be approximately 1-2 cm2. For intestine, take approximately 3 cm long segments and partially open longitudinally with scissors (to allow formalin to contact the mucosal surface).
Test list (for AHL use - included on submission form):
Virology: PRRSV PCR prtqn, influenza A virus PCR inflpcr: lung (pool)
Mycoplasmology: M.hyopneumoniae PCR mhpcr: lung (pool)
Bacterial culture bsetup, cults: lung (pool).
Histopathology hist
Freeze and hold: tonsil /spleen, lung, kidney /liver, lymph nodes, brain.