Gastrointestinal Disease in Grower-Finisher Pigs

Required Forms:

TISSUE SAMPLING LIST-collect the following samples from EACH pig

Fresh tissues: 1 sample per Whirl-Pak bag, per pig – label with stickers provided.

Formalin-fixed tissues: 1 sample jar per pig.

Tissue	Fresh (include lesions)	In Formalin (include lesions)
Tonsil	X	X
Thyroid gland		X
Trachea		X
Thymus		X
Lung	X 2	X
Heart		X
Liver	X	X
Spleen	X	X
Kidney	X	X
Adrenal gland		X
Pancreas		X
Mesenteric lymph node	X	X
Gastrohepatic lymph node	X	X
Stomach		X
Duodenum		X
Jejunum		X
Ileum	X 4	X
Spiral colon	X 3	X
Feces	X
Diaphragm and skeletal muscle		X
Brain	X half, sagitally sectioned (OR intact head, fresh)	X half, sagitally sectioned (OR intact head, fresh)

Gastrointestinal Disease in Grower-Finisher Pigs (>20 kg)

If <40 kg each, may include up to 3 pigs per case

Fresh tissues: Use a separate Whirl-Pak bag for each sample –label bags with the stickers provided (tissue name), and add a pig designation (A, B, C – if >1 pig).

Formalin-fixed tissues: Use 1 sample jar per pig and label with the pig designation (A, B, C). For solid organs, samples should be approximately 1-2 cm². For intestine, take approximately 3 cm long segments and partially open longitudinally with scissors (to allow formalin to contact the mucosal surface).

Test list (for AHL use - included on submission form):

Virology: PRRSV PCR prtqn, influenza A virus PCR inflpcr: lung (pool)

Rotavirus A/B/C PCR rotapcr and porcine coronavirus PCR pcovpcr: ileum (pool)

Bacterial culture bsetup, cults: ileum, colon (pool by pig)

Lawsonia PCR lapcr: ileum (pool)

Brachyspira spp PCR brpcr AND bspcr: colon (pool)

Parasitology – fecal flotation fflot: feces (pool)

Histopathology hist

Freeze and hold: tonsil / spleen, lung, kidney / liver, lymph nodes, ileum, colon, brain.