Respiratory disease

Specimen type/volume 

Collection protocol

Bacteriology

• Bacterial culture, swine (cults)

• affected areas of lung
• pleural fluid/swab

Histology

• Histopathology, food animal (hist)
• Immunohistochemistry is available on formalin-fixed tissues for PRRSV, PCV-2, influenza A virus

Formalin fixed:

• sections of SEVERAL pieces of lung from cranial and caudal lobes, including borders of affected and unaffected areas
• trachea, bronchial lymph node

• M. hyopneumoniae PCR and molecular typing (mhpcr) – note: M. hyopneumoniae cannot be cultured

• lung, oral fluids

• M. hyorhinis PCR (hrhpcr)
• Mycoplasma culture (mculf)

• lung

Serology

• PRRSV (prrx3)
• Influenza A virus (H1N1, H3N2) HI (h1n1hi, h3n2hi)
• M. hyopneumoniae (mheiadx)
• Actinobacillus pleuropneumoniae (xappmul) – APP serology is only useful on a herd basis, please phone the laboratory before submitting large numbers of sera
• PRCV (pcve2) – antigenically indistinguishable from TGEV

• serum samples from animals of various age groups
• consult lab for submission details – e.g., the number of samples to be submitted, age of animals to be submitted

Virology

• Influenza A PCR (inflpcr)
• PRRSV PCR (prtqn)
• M. hyopneumoniae PCR (mhpcr)
• PCV-1,2,3 PCR (pcv123)
• Atrophic rhinitis (pmtpcr)

• lung, tonsil
• nasal swabs in virus transport medium
• submission of nasal swabs from several pigs is necessary
• consult laboratory for numbers and to obtain swabs (provided for a fee) for testing

Specimen type/volume

Collection protocol

Includes: (brsppnl)

• bovine viral diarrhea virus
• bovine adenovirus
• bovine coronavirus
• bovine respiratory virus panel PCR (BOHV-1/IBR, BPIV-3, BRSV)
• Mycoplasma bovis PCR
• bacterial culture

• two samples needed per test
• PCR – collect swabs with virus transport medium (VTM) or a swab in 2mL of sterile saline in a red-top tube
• tissue in leakproof container
• may pool up to 5 samples – indicate pooling instructions on submission form
• bacterial culture – swab in bacterial medium or lung tissue, no pooling
• if pooling is requested, individual tests of the panel will be ordered rather than the panel
• AHL splitting charges may apply

• Bacterial culture, food animal (cultf)

• affected areas of lung, pleural fluid/swab, tracheobronchial aspirate, bronchoalveolar lavage fluid

• Histology, food animal (hist)
• Immunohistochemistry is available on formalin-fixed tissues for BVDV, BCV, BoHV-1 (IBRV), BRSV

• Formalin-fixed sections of SEVERAL pieces of lung from cranial and caudal lobes, including borders of affected and unaffected areas, trachea, bronchial lymph nodes

• Mycoplasma bovis PCR (mbpcr) and/or culture (mculf)

• lung from the borders of pneumonic areas
• deliver or freeze immediately

• Baermann (baer) for lungworm larvae

• feces

• BoHV-1 (IBRV) ELISA (ibre)
• BPIV-3 (pi3)
• BRSV (brs)
• bovine coronavirus (bcv)
• BVDV 1 and 2 (bvdn, bvds, bvd2)
• bovine adenovirus (bav3)

• submit paired sera from affected animal and/or survivors/herdmates

• BVD PCR (bvdrt)
• BoHV-1/IBRV PCR (ibrrt)
• BRSV PCR (part of brvp3 panel)
• bovine coronavirus PCR (bvdadco)
• bovine parainfluenza 3 virus PCR (part of brvp3 panel)

• Note: BoHV-1 (IBRV), BRSV and PI3 are included in the bovine respiratory panel PCR (brvp3)

• lung, trachea
• nasal swabs

Specimen type/volume

Collection protocol

• Bacterial culture, food animal (cultf)

• affected areas of lung, pleural fluid/swab

• Histology, food animal (hist)
• Immunohistochemistry is available for small ruminant lentiviruses (CAEV, MVV)

• Formalin-fixed sections of SEVERAL pieces of lung from cranial and caudal lobes, including borders of affected and unaffected areas, trachea, bronchial lymph node

• Mycoplasma culture (mculf)

• frozen or fresh lung

• Baermann (baer) for lungworm larvae

• feces

• OPP, CAEV ELISA (mvveh)

• serum

• Bovine respiratory PCR panel (brvp3)

• lung, nasal swab

Specimen type/volume

Collection protocol

• Bacterial culture, aerobic (cultnm or cultn) or anaerobic (anculnm or ancultn)

• affected areas of lung, pleural fluid/swab, tracheobronchial aspirate, or bronchoalveolar lavage fluid

• Histopathology (histcm3)
• IHC, Equid herpesvirus 1 (ehvi)

Formalin fixed:

• SEVERAL pieces of lung from cranial and caudal lobes, including borders of affected and unaffected areas, trachea, bronchial lymph node

• Mycoplasma culture (mculn)

• lung (fresh or frozen), pleural fluid

• Equine respiratory serology panel (respe) includes:
  • Equid herpesvirus 1/4 VN
  • Equid herpesvirus 2 VN
  • Equine rhinitis A VN
  • Equine rhinitis B VN
  • H7N7 influenza HI
  • H3N8 influenza HI

• serum (paired samples)

• Equid herpesvirus 1 PCR (ehv12)
• Influenza A matrix PCR (inflpcr)

• lung, bronchial lymph node, nasal/pharyngeal swabs

Specimen type/volume

Collection protocol

Includes testing for:

• Bordetella bronchiseptica
• Strepococcus equi ssp. Zooepidemicus
• Canine adenovirus 1/2
• Canine herpesvirus 1
• Canine parainfluenza virus
• Canine distemper virus
• Canine respiratory coronavirus
• Influenza A virus matrix PCR
• Mycoplasma culture (e.g. M. cynos, M. canis, M. edwardii)

• Nasal swab (eswab only) not gel swab AND virus transport medium swab.
• Pleural fluid, tracheobronchial aspirate, or bronchoalveolar lavage fluid, - split into 3 separate sterile vials for distribution to the lab sections, if done at AHL an extra splitting charge will be applied
• Lung

Includes testing for:

• Bordetella bronchiseptica
• Streptococcus equi ssp. zooepidemicus
• Feline calicivirus
• Feline herpesvirus 1
• Influenza A virus matrix PCR
• Mycoplasma culture (e.g. M. felis, M.gateae)

• Nasal swab (eswab only) not gel swab AND virus transport medium swab.
• Pleural fluid, tracheobronchial aspirate, or bronchoalveolar lavage fluid, - split into 3 separate sterile vials for distribution to the lab sections, if done at AHL an extra splitting charge will be applied
• Lung

• Bacterial culture, aerobic, companion/other (cultnm or cultn) or anaerobic culture (anculnm or ancultn)

• Affected areas of lung, pleural fluid/swab, tracheobronchial aspirate, or bronchoalveolar lavage fluid

• Histopathology, companion/other (histcm3)
• Immunohistochemistry is available on formalin-fixed tissues for canine distemper virus (CDV), feline coronavirus (FIP), feline herpesvirus, influenza A virus

Formalin fixed:

• sections of SEVERAL pieces of lung from cranial and caudal lobes, including borders of affected and unaffected areas
• trachea, bronchial lymph node
• heart, liver, kidney, spleen

• Influenza A virus ELISA (aifem)
• Canine adenovirus (CAdV) VN (cav12)
• Canine parainfluenza virus (CaHV) VN (cpi)

• paired sera

• Canid herpesvirus 1 (CaHV-1), canine adenovirus 2 (CAdV-2), canine parainfluenza virus (CPIV) triplex PCR (chappcr)

• live: nasal swab
• dead: kidney, liver, lung, spleen

• Canine distemper virus (CDV) PCR (cdvmb)

• lung, retropharyngeal or bronchial lymph nodes, kidney, bladder, brain
• tonsillar or conjunctival scrapings

• Feline calicivirus, felid herpesvirus PCR panel (fcfhpcr)

• live: nasal swab, conjunctival swab
• dead: lung, trachea

• Influenza A virus PCR (inflpcr)

• lung
• tonsillar or conjunctival scrapings