Program Lead, Host & Pathogen Determinants
Laboratory for Foodborne Zoonoses
Public Health Agency of Canada
110 Stone Road West
Guelph, Ontario N1G 3W4
B.Sc. - University of British Columbia
M.Sc. - University of British Columbia
Ph.D. - University of British Columbia
My laboratory is actively sequencing the genomes of bacteriophages, with the goals of understanding virus evolution and the construction of useful vector systems. Towards this end we have sequenced a number of phages infecting Escherichia coli (T1, rv5), Pseudomonas (D3, F116, gh-1) and Salmonella (P22) species. Recently we have completed the sequence of the first bacteriophage (BCJA1c) active on an alkaliphilic bacterium. I have also contributed to the annotation of two Vibrio (VP2, VP5) and two Salmonella (ε15, ε34) phages. Coliphage T7 is one of the best studied bacteriophages and one of the first to be completely sequenced. The T7 sensu strictu group of phages is composed of phages T7, T3, φYeO3-12, φA1122 and gh-1 which are lytic phages sharing all essential genes in common. Evolution of this group of viruses most likely proceeds through the initial acquisition of host range mutations followed by genetic ìadjustmentî (known as amelioration) to the biochemical environment of the new host. I am interested in isolating and analyzing other members of this group of viruses.
I am interested in the cytochemistry of spirochetes, in particular the structure and function of their outer membranes. Work with Spirochaeta aurantia has shown the presence of several novel glycolipids rather than lipopolysaccharide. The sequence of one of these has been recently published.
Byrne, M. and A.M. Kropinski. (2005) The genome of the Pseudomonas aeruginosa generalized transducing bacteriophage F116. Gene 346: 187-194.
Kropinski, A.M., Hayward, M., Agnew, M.D. and Ken F. Jarrell. (2005). The genome of BCJA1c, a bacteriophage active against the alkaliphilic bacterium, Bacillus clarkii. Extremophiles 9: 99-109.
Vinogradov, E., Paul, C.J., Li, J., Zhou, Y., Lyle, E.A., Tapping, R.I., Kropinski, A.M. and M.B. Perry. (2004) The structure and biological characteristics of Spirochaeta aurantia outer membrane glycolipid LGL B . Eur. J. Biochem. 271: 4685 - 4695.
McLaughlin, R., Secko, D.M., Paul, C.J., and A.M. Kropinski. (2004) Nature of the genome of the saprophytic spirochete Spirochaeta aurantia and its ribosomal RNA operons. Can. J. Microbiol. 50: 967-971.
Roberts, M.D., Martin, N.L. and Kropinski, A.M. (2004) The genome and proteome of coliphage T1. Virology 318: 245-266.
Kovalyova, I.V. and Kropinski, A.M. (2003) The complete genomic sequence of lytic bacteriophage gh-1 infecting Pseudomonas putida - evidence for close relationship to the T7 group. Virology 311: 305-315.
Vander Byl., C. and A.M. Kropinski. (2000) Sequence of the genome of Salmonella bacteriophage P22. J. Bacteriol. 182: 6472-6481
Kropinski, A.M. (2000) Sequence of the genome of the temperate, serotype-converting, Pseudomonas aeruginosa bacteriophage D3. J. Bacteriol. 182: 6066-6074.