Keith Warriner gained a 1st Class BSc (Hons) in Food Science specializing in food microbiology from Nottingham University, UK and PhD in Microbial Physiology at the University of Wales Aberystwyth. In 1994 he joined the University of Manchester and developed reagentless sensors designed for routine monitoring on analytes outside of the laboratory environment. Keith subsequently joined the Division of Food Sciences at Nottingham University in 1997. Here work included the development and validation of UV-lasers for package sterilization, DNA-fingerprinting to identify of cross-contamination points in pork processing and the interaction of human pathogens with growing salad vegetables/sprouted seeds. He joined the Department of Food Science within the University of Guelph in 2002 as an Assistant Professor in food microbiology. Current projects include studying the interaction of human pathogens with growing plants, novel decontamination methods in the fresh produce industry, molecular epidemiology of enteric contamination within meat chains and development of reagentless biosensors for biohazard detection.
Dr Warriner has broad food safety research interests that encompass epidemiology, intervention methods, diagnostics and relevant to the current proposal, the application of biocontrol preparations to reduce the prevalence of human pathogens at the primary production level. In previous studies the applicants have evaluated the efficacy of bacteriophages to control the prevalence of Salmonella in pig production. Here, bacteriophages were administered to Salmonella infected pigs, in addition to being sprayed in the environment. The conclusion of the study was that phages could reduce but not eliminate Salmonella associated with pigs. In more recent research, the applicant has developed an effective method based on using a combination of bacteriophages and antagonistic bacteria to control Salmonella on fresh produce (sprouted seeds and tomatoes).
OMAFRA OMAFRA:Partnership OCE NCFPD Development of a Novel UV Reactor for Non-thermal Pasteurization Raw Milk Prevalence of Clostridium difficile in pork production and processing Extraction and Detection of Biohazards
Food Microbiology Industrial Microbiology
Chapters in books Warriner K Food Microbiology. In: Introduction to Food Science. Ed. R.Y. Yada and A.R. Hill. Thomson Delmar Learning USA (In preparation). Keith Warriner, Edward P. C. Lia, Azadeh Namvar, Daniel M. Hawkins and Subrayal M Reddy. Molecular Imprinted Polymers for Biorecognition of Bioagents In: Handbook of Bacterial detection: Biosensors, recognition receptors and microsystems.Ed:. Mohammed Zourob and Tony Turner. Springer Scientific. Warriner K. Pathogens in vegetables 2006. In: Raw material safety: fruit and vegetables. Ed W. Jogen Woodhead Publishing, London, UK pp 3-43. Warriner, K. Laser-based packaging sterilization in aseptic processing. 2004 In: Improving Therma P. Richardson (ed). Woodhead Publishing, London, UK pp 277-304. . Warriner K and Zivanovic S.Microbial metabolites in fruit and vegetables. In:Produce Degradation: Reaction Pathways and their Prevention. Ed O Lamikanra, S Imam and D O Ukuku. CDC Press, USA. pp 505-528. Warriner, K., Christie, I.M., Rigby, G. P., Treloar, P., Maines, A., Ashworth, D., Eddy, S., Reddy, M. and Vadgama, P. (1998) The use of chemical sensor systems for sampling, selectivity and speciation. In: Biosensors for Direct Monitoring of Environmental Pollutants in Field. Kluwer Academic Pub. (NE), pp. 41-56. iii) Papers, letters to editors and technical notes published in refereed journals *Graduate students either supervised* or co-supervised** In Review Ye, J*., M. Kostrzynska, K. Dunfield and K. Warriner. Control of Salmonella on sprouting mung beans by using a combination of antagonistic bacteria and lytic bacteriophages. J Food Prot. (In review JFP-09-063). Wang, S*., Zhoa, W*., Namvar, A*., Johnson, R., Friendship, R and Warriner, K. Prevalence of Salmonella infecting bacteriophages associated with Ontario pig farms and the holding area of a high capacity pork processing facility. J Sci Food Agri (In review JSFA-09-0338). Ye, J., M. Kostrzynska, K. Dunfield and K. Warriner Evaluation of a biocontrol preparation consisting of Enterobacter asburia JX1 and a lytic bacteriophage cocktail to suppress the growth of Salmonella Javiana associated with tomatoes. J Food Prot. (In Press, JFP 09-097R) 2009 Warriner, K, Dunfield K, Namvar, A*. and Hubard, A. Sources and Interaction of Human Pathogens with Fresh Produce. Advances in Food and Nutrition Research (In Press). Shi, X*., Namvar, A*., Kostrzynska, M., Dunfield, K. and Warriner, K. Warriner K and Namvar A*. What is the hysteria with Listeria? Trends in Food Science and Technology 20; 245-254. Shi, X*., Namvar, A*., Kostrzynska, M., Dunfield, K. and Warriner, K. Microbial Population Profiles of the Microflora Associated with Pre- and Post-harvest tomatoes contaminated with Salmonella Typhimurium or Salmonella Montevideo. J. Appl Microbiol. 107; 329-338. 2008 Dadarwal, R*., Namvar, A*., Thomas, D, Hall JC and Warriner K (2008). Organic Conducting Polymer Electrode Based Sensors for Detection of Salmonella Infecting Bacteriophages. Materials Science and Engineering (In Press MSC2342). McEgan R*, Fu TJ and Warriner K (2009). Pathogen screening of spent irrigation water in sprout production using tangential flow filtration coupled with an immuno-sensor. Journal of Food Protection . Klotz, V**., Hill, A., Warriner, K., Griffiths, M. and Odumeru, J. (2008). Assessment of the colorimetric and fluorometric assays for alkaline phosphatase activity in cow's, goat's, and sheep's milk. J Food Prot 71, 1884-1888. McEgan R*., Lee S, Schumacher B and Warriner K (2008). Composite versus Single Sampling of Spent Irrigation Water to Access the Microbiological Status of Sprouting Mung Bean Beds. JAFS 88, 1549-1553. Xie, Y*., Hajdok, C*., Mittal, G. and Warriner K. (2008). Inactivation of MS2 (F+) coliphage on lettuce using a combination of ultraviolet light and hydrogen peroxide. J. Food Prot. 71, 903-907. Hajdok, C*., Mittal, GS and Warriner K. (2008) Decontamination of fresh produce using a combination of ultraviolet light and hydrogen peroxide. J. Appl Microbiol. 104: 1014-1024 2007 Shia. X*, Namvar, A*., Hora, R*., Kostrzynska, M. and Warriner, K. (2007). Interaction of Salmonella with pre- and post harvest tomatoes. J. Food Prot. 70: 2725-2731. M. Kumar,* R. Hora*, M. Kostrzynska and K. Warriner. (2007) Mode of Salmonella and Escherichia coli O157:H7 Inactivation by an Stabilized Oxychloro-based Sanitizer. J. Appl. Microbiol. 44: 188-193. Namvar A*and Warriner K.(2007). Microbial Imprinted Polypyrrole and poly3-methylthiophene Composite Films for the Detection of Bacillus Endospores. Biosensors and Bioelectronics 22 (9-10): 2018-2024 M. Kumar,* R. Hora*, M. Kostrzynska and K. Warriner (2007). Inactivation of Escherichia coli O157:H7 and Salmonella on Naturally Contaminated Mung Beans (Vigna radiata) using a Stabilized Oxychloro-based Sanitizer. Lett Appl Microbiol. 44: 188-193 2006 Kumar, M.*, Hora, R.*, Kostrzynska, M., Waites W. M. and Warriner K. (2006). Inactivation of Escherichia coli O157:H7 and Salmonella on Mung Beans, Alfalfa and other Seed Types Destined for Sprout Production by Using an Oxychloro-Based Sanitizer. J. Food Prot. 69 (7): 1571-1578 A. Namvar* and K Warriner (2006). Application of Enterobacterial repetitive intergenic consensus-Polymerase Chain Reaction to Trace the Fate of Generic Escherichia coli and Salmonella within a High Capacity Pork Slaughter Line Employing a Triple Singeing Step. Int J Food Microbiol. 108, 2, 155-163. A. Farzan**, R M. Friendship, C E. Dewey, K Warriner, C Poppe and K Klotins (2006). The prevalence of Salmonella spp based on fecal culture and serum antibody from pigs on farms using liquid or dry feeding. Preventive Veterinary Medicine 73:241-54. 2005 Kumar, M.*, Hora, R.*, L. Garcia, B Schumacher, J Odumeru and K. Warriner (2005). Spatial Distribution of Salmonella, Escherichia coli O157:H7 and other Bacterial Populations in Commercial and Laboratory Scale Sprouting Mung Bean Beds. J. Food Prot. 68: 2510-2518. A. Namvar* and K Warriner . Attachment Strength to Pork Skin, Quaternary Ammonium Salt and Thermal Resistance of Generic Escherichia coli Isolates Recovered from a Pork Slaughter Line. J. Food Prot. 68; 2447-2450. Rajneesh Hora**, Keith Warriner, Barry J. Shelp and Mansel W. Griffiths. Effect of Biological and Mechanical Disruption of Growing Spinach Plants on the Internalization of Escherichia coli O157:H7. J. Food Prot 68; 2506–2509. Jiali Xu* and Keith Warriner: Coliphage as Indicators of Fecal Contamination in Hydroponic Cucumber (Cucumis sativus L.) Greenhouses. J Sci Food Agri. 85: 2397-2400. Keith Warriner, Faozia Ibrahim*, Matthew Dickinson, Charles Wright and William M. Waites (2005). Seed Decontamination as an Intervention Step for Eliminating Escherichia coli on Salad Vegetables and Herbs. J Sci Food Agri 85: 2307-2313. Jablasone, J*., Warriner, K and Griffiths, M W.(2005). Interaction of Escherichia coli O157:H7, Salmonella Tyhimurium and Listeria monocytogenes with a Range of Growing Plants Cultivated in a Gnotobiotic system. Int. J. Food Microbiol. 99: 7-18. Waites WM and Warriner K (2005). Ultra-violet Sterilisation of Food Packaging. Culture 26: 1-3. 2003 Warriner K, Ibrahim, F*., Dickinson, M., Wright, C. and Waites W. M. (2003). Internalization of human pathogens within growing salad vegetables. Reviews in Molecular Biology and Biotechnology 20; 117-134.. Warriner K, Ibrahim, F*, Dickinson, M., Wright, C. and Waites W. M. (2003). Interaction of Escherichia coli with Growing Salad Spinach Plants. J. Food Prot. 66; 1790-1797. Warriner, K., Spaniolas, S*., Dickinson, M, Wright, C. and Waites, W. M. (2003) Interaction of Bioluminescent Escherichia coli and Salmonella Montevideo with Growing Bean Sprouts J. Appl. Microbiol. 95; 719-727. 2002 Warriner, K, Kolstad, J., Rumsby, P.and Waites, W. M. (2002) Carton Sterilisation by U.V.-C Excimer Laser Light: Recovery of Bacillus subtilis Spores on Vegetable Extracts and Food Simulation Matrices. J. Appl. Microbiol. 92: 1051-1057 Warriner, K., Aldsworth, T. G., Kumar, S*. and Dodd, C. E. R (2002). Cross-Contamination of Carcasses and Equipment During Pork Processing. J. Appl. Microbiol. 93: 169-177. Warriner, K. , A. Morrissey, G. King, P. M. Vadgama, , J. Alderman, P. Treloar and J. B. Pourciel. (2002) Electrochemical sensors for in-line monitoring of ethanol in fermentation processes. Sensors and Actuators 84, 200-207. 2001 Warriner, K., Eveleigh, K., Goodman, J., Betts, G., Gonzales, M. and . Waites, W. M. (2001). Attachment of bacteria and a bioluminescent labeled Escherichia coli to steam pasteurized beef carcasses J. Food Prot. 64: 493-497. 2000 Warriner, K ., Rysstad, G., Murden, A., Rumsby, P., Thomas, D. and Waites, W. M. (2000). Inactivation of Bacillus subtilis Spores on Aluminum and Polyethylene Pre-formed Cartons by UV-Excimer Laser Irradiation. Journal Food Prot. 63: 753-757. 1999 Warriner, K and W. M. Waites (1999). Enhanced sporulation in Bacillus subtilis grown on medium containing glucose:ribose. Lett. Appl. Microbiol. 29: 97-102. 1997 Warriner, K., Higson, S., Ashworth, D., Christie, I. and Vadgama, P. (1997) Stability of Dodecyl Sulphate-doped Poly(pyrrole)/Glucose Oxidase Modified Electrodes Exposed to Human Blood Serum. Materials science and Engineering C5, 81-90. Warriner, K., Higson, S. and Vadgama, P. (1997) Amperometric Pyruvate Sensor Based on Regeneration of NADH at a Poly(3-methylthiophene)/Poly(phenol red) Modified Electrode. Materials science and Engineering C5, 91-99. Warriner, K., Higson, S., Ashworth, D., Christie, I. and Vadgama, P. (1997) Stability of Dodecyl Sulphate-doped Poly(pyrrole)/Glucose Oxidase Modified Electrodes Exposed to Human Blood Serum. Materials science and Engineering C5, 81-90. Warriner, K., Higson, S. and Vadgama, P. (1997) Amperometric Pyruvate Sensor Based on Regeneration of NADH at a Poly(3-methylthiophene)/Poly(phenol red) Modified Electrode. Materials science and Engineering C5, 91-99. 1995 Warriner, K. and Morris, J. G. (1995) The Effects of Aeration on the Bioreductive Abilities of Some Heterofermentative Lactic Acid Bacteria. Lett. Appli. Microbiol. 20: 323-327. Eddy, S., Warriner, K., Christie, I., Ashworth, D. and Vadgama, P. (1995) Electropolymerization and Other Surface Modifications for Practical Measurements. Biosensors and Bioelectronics, 10: 831-839. Warriner, K., Higson, S., Christie, I., Ashworth, D. and Vadgama, P. (1995) Electrochemical Characterization of Two Model Electropolymerised Films for Enzyme Electrodes. Biosensors and Bioelectronics, 11: 615-624.
Biohazards through the Food Chain. Food Safety Symposium. Toronto Oct 09 Biocontrol of Salmonella on Pre- and Post-Harvest Tomatoes, IAFP Texas Aug 09 Food Safety in the health care setting. CHICA Eastern Ontario meeting. Kingston, ON Feb 09. (Invited). Listeria, C. difficile, MRSA - The Foodborne Link. Webber Scientific Teleclass. Feb 09 (Invited). Control of Salmonella in pigs using bacteriophage. Centralia Swine Research Update (CSRU), London, ON. Jan 09. (Invited). Interaction of Salmonella with pre- and post-harvest tomatoes. ILSI annual meeting. Tuscan, Arizona. Jan 09 (Invited). Listeria monocytogenes in RTE foods. Canadian Food Safety Institute Oct 2008. Toronto. (Invited)