Molecular and Cellular Imaging Basics

Light Microscopy

Light microscopes provide a highly magnified image of an object and allow us to visualize and measure fine details at the cellular and even subcellular level.  The microscopic image is produced in 2 steps: the objective forms a real, inverted, magnified image (=intermediate image), which is then further enlarged to a virtual image by the oculars.  Transmitted light microscopy employs various optical/physical techniques to produce an image: regular bright field, dark field (oblique illumination of the object), polarized light, phase contrast, and differential inter