Genomics Services

Agilent BioAnalyzer 2100

Please note, Bioanalyzer Service has been replaced with the TapeStation Service.

 

 

 

Equipment Used: Agilent TapeStation 4150

Agilent TapeStation 4150 Service

The TapeStation system allows for automated electrophoresis of most DNA and RNA samples. Typical applications include quality control (QC) of NGS libraries and RNA extractions (ie. RIN).  This method excels in the determination of fragment length distribution of nucleic acid samples, but is not typically relied on for accurate quantification.  The TapeStation can be used in place of the BioAnalyzer for most applications, but cannot currently be used for small/micro RNA analysis.

Service includes Standard and High Sensitivity assays for RNA, DNA >1kb, and DNA >5kb, as well as a Standard gDNA assay.

Request Form: AAC TapeStation Request Form

 

Equipment Used: Agilent TapeStation 4150

DNA Sequencing

Sample submission considerations.

The facility provides a number of standard sequencing primers for common plasmid vectors.
Custom primers should be provided at a concentration of 5 uM (=5 pmol/uL).

Request Forms:
Individual Sample Submissions
Sanger Sequencing Request - Use this form to submit DNA templates (ie. PCR amplicons, Plasmids, etc.) for sequencing
DIY Sequencing Request - Use this for to submit completed flourescent Sanger products (ie. BigDye reactions) for sequencing

For full and half-plate submissions please contact the facility

Equipment Used: Applied Biosystems 3730 DNA analyzer

Next Generation Sequencing using MiSeq desktop sequencer

  • Metagenomics
  • Small genome sequencing
  • Amplicon sequencing
  • Targeted gene sequencing

Equipment Used: Illumina MiSeq Sequencer

Nucleic Acid Fluorometry

Fluorometric concentration assays are generally more accurate than spectrophotometric/UV-Vis absorbance (ie. Nandrop) measurements because they are less affected by common contaminants (ie. Proteins, RNA, or DNA).

This method is recommended NGS library assessments and for any application that requires higher accuracy in concentration estimates than can be acheived with Nanodrop.

Request Form:
Qubit Request

 

Equipment Used: Qubit® 2.0 Fluorometer