Agilent TapeStation 4150 Service

The TapeStation system allows for automated electrophoresis of most DNA and RNA samples. Typical applications include quality control (QC) of NGS libraries and RNA extractions (ie. RIN).  This method excels in the determination of fragment length distribution of nucleic acid samples, but is not typically relied on for accurate quantification.  The TapeStation can be used in place of the BioAnalyzer for most applications, but cannot currently be used for small/micro RNA analysis.

Service includes Standard and High Sensitivity assays for RNA, DNA >1kb, and DNA >5kb, as well as a Standard gDNA assay.

Request Form: AAC TapeStation Request Form

 

Equipment Used: Agilent TapeStation 4150

DNA Sequencing

Sample submission considerations.

The facility provides a number of standard sequencing primers for common plasmid vectors.
Custom primers should be provided at a concentration of 5 uM (=5 pmol/uL).

Request Forms:
Individual Sample Submissions
Sanger Sequencing Request - Use this form to submit DNA templates (ie. PCR amplicons, Plasmids, etc.) for sequencing
DIY Sequencing Request - Use this for to submit completed flourescent Sanger products (ie. BigDye reactions) for sequencing

For full and half-plate submissions please contact the facility

Equipment Used: Applied Biosystems 3730 DNA analyzer

Fragment Analysis

Information on sample submission and how it works.

Equipment Used: Applied Biosystems 3730 DNA analyzer

Gene Expression Services

Request Forms:
Digital Droplet PCR Request
Real-Time PCR Registration
Real-Time PCR Request

Equipment Used:
BioRad Qx200 Droplet Digital PCR (ddPCR) System
QuantStudio 7 Pro Real-Time PCR System

Next Generation Sequencing

The Illumina NextSeq 1000 is an NGS sequencing system designed to miniaturize sample and reagent volumes and increase read output. The NextSeq 1000 is capable of many sequencing applications, including targeted metagenomics, whole genome sequencing, transcriptomics, immune repertoire profiling, sequencing of targeted panels, exomes and large panels, circulating tumor DNA, single-cell sequencing, and spatial profiling.

Contact the facility to discuss services and applications.

Equipment Used: Illumina NextSeq 1000 Sequencer

Nucleic Acid Fluorometry (Qubit)

Fluorometric concentration assays are generally more accurate than spectrophotometric/UV-Vis absorbance (ie. Nandrop) measurements because they are less affected by common contaminants (ie. Proteins, RNA, or DNA).

This method is recommended NGS library assessments and for any application that requires higher accuracy in concentration estimates than can be acheived with Nanodrop.

 

Request Form:
Qubit Request

 

Equipment Used: Qubit Flex Fluorometer, Qubit® 2.0 Fluorometer

PromethION Services

Plasmid & PCR Amplicon Sequencing

The PromethION 24 is a high-throughput nanopore sequencer capable of natively sequencing both RNA and DNA with rapid turn-around times. The PromethION can load up to 24 flow cells, each capable of multiplexing 96 samples or more. It is also possible to re-use flow-cells, decreasing cost and waste. The PromethION 24 is suitable for long-read applications, including genome assembly, native methylation seq., direct RNA transcript sequencing (including alternate splicing), and metagenomics.

We have phased out Sanger Sequencing in favor of long-read sequencing on the Oxford Nanopore PromethION 24. The facility provides sequencing of DNA fragments up to 25 kilobases in length or more, including whole plasmids and PCR amplicons. Service starts on September 2.

  • Sequenced at the University of Guelph: no tax or shipping fees for internal clients
  • Plasmid analysis run on Tuesdays and Thursdays with next-day turnaround
  • Pricing includes bioinformatic report, plasmid annotation, insert variant calling, and multiplasmid/contamination detection.
  • See our detailed sample report
SERVICE INTERNAL ACADEMIA COMMERCIAL
Whole Plasmid $19.50 $19.50 $25
PCR Amplicon $19.50 $19.50 $25
Batch of 96 Plasmid/Amplicon $1,872 $1,872 $2,400

Sequencing Request Form - Use this form to submit DNA templates (e.g. PCR amplicons, Plasmids) for sequencing. Recommended at least 100 ng DNA. Recommended concentration 10 ng/µL in 10 µL in molecular grade water or TE buffer. Concentration should be estimated by fluorometric methods such as Qubit/PicoGreen.

  1. Fill out all three tabs of the request form (first two tabs are mandatory)
  2. Signed request form emailed to genomics@uoguelph.ca
  3. Drop the samples off at room SSC 1401 with facility staff or in the Sample Drop-Off Freezer

Other PromethION Services:

The PromethION is suitable for a range of other services, including native Methylation sequencing (or other modified bases), direct RNA sequencing, 16S profiling (or 18S/ITS/other amplicon metabarcoding), transcriptomics, whole genome sequencing, metagenomics, genome skimming, variant calling, and much more.

SERVICE INTERNAL ACADEMIA COMMERCIAL
Whole Flow Cell $1200 $1200 $1500
Other applications Contact us for quote

Equipment Used: Oxford Nanopore PromethION 24