Genomics Services

DNA Sequencing

Sample submission considerations.

The facility provides a number of standard sequencing primers for common plasmid vectors.
Custom primers should be provided at a concentration of 5 uM (=5 pmol/uL).

Request Forms:
Individual Sample Submissions
Sanger Sequencing Request - Use this form to submit DNA templates (ie. PCR amplicons, Plasmids, etc.) for sequencing
DIY Sequencing Request - Use this for to submit completed flourescent Sanger products (ie. BigDye reactions) for sequencing

For full and half-plate submissions please contact the facility

Equipment Used: Applied Biosystems 3730 DNA analyzer

Next Generation Sequencing using MiSeq desktop sequencer

  • Metagenomics
  • Small genome sequencing
  • Amplicon sequencing
  • Targeted gene sequencing

Equipment Used: Illumina MiSeq Sequencer

Nucleic Acid Fluorometry

Fluorometric concentration assays are generally more accurate than spectrophotometric/UV-Vis absorbance (ie. Nandrop) measurements because they are less affected by common contaminants (ie. Proteins, RNA, or DNA).

This method is recommended NGS library assessments and for any application that requires higher accuracy in concentration estimates than can be acheived with Nanodrop.

Request Form:
Qubit Request

 

Equipment Used: Qubit® 2.0 Fluorometer

RNA/DNA Analysis using Agilent BioAnalyzer 2100

Please note, the Bioanalyzer is currently being phased out as we prepare to launch the new TapeStation service.

Request Form: 
Bioanalyzer Request

Equipment Used: Agilent 2100 Bioanalyzer