The facility provides a number of standard sequencing primers for common plasmid vectors.
Custom primers should be provided at a concentration of 5 uM (=5 pmol/uL).
Individual Sample Submissions
Sanger Sequencing Request - Use this form to submit DNA templates (ie. PCR amplicons, Plasmids, etc.) for sequencing
DIY Sequencing Request - Use this for to submit completed flourescent Sanger products (ie. BigDye reactions) for sequencing
For full and half-plate submissions please contact the facility
Equipment Used: Applied Biosystems 3730 DNA analyzer
Next Generation Sequencing using MiSeq desktop sequencer
- Small genome sequencing
- Amplicon sequencing
- Targeted gene sequencing
Equipment Used: Illumina MiSeq Sequencer
Nucleic Acid Fluorometry
Fluorometric concentration assays are generally more accurate than spectrophotometric/UV-Vis absorbance (ie. Nandrop) measurements because they are less affected by common contaminants (ie. Proteins, RNA, or DNA).
This method is recommended NGS library assessments and for any application that requires higher accuracy in concentration estimates than can be acheived with Nanodrop.
Equipment Used: Qubit® 2.0 Fluorometer