Prices listed are predominantly for researchers at the University of Guelph (2018). Fees are subject to change without notice.
Please contact genomics@uoguelph.ca to inquire about fees for external clients.
Agilent TapeStation 4150 Fees
| Assay | Set-up | Per Sample |
|---|---|---|
| RNA | $10.50 | $5.50 |
| HS RNA | $12.00 | $6.25 |
| D1000 | $8.00 | $5.50 |
| HS D1000 | $11.00 | $7.75 |
| D5000 | $12.75 | $5.25 |
| HS D5000 | $17.25 | $7.75 |
| gDNA | $8.75 | $8.75 |
Set-up fee is a flat fee charged per submission in addition to the per sample charge.
Set-up fee covers DNA/RNA ladders, technician time, and instrument overhead. Contact facility for more information about ladders and when to use them.
Annual Subscriptions
| SERVICE | INTERNAL |
|---|---|
| Nanodrop | $150 per year |
| QuantStudio 7 Pro self-service | $150 per year for registration and $7 per plate run |
| Nanodrop & QuantStudio 7 Pro | $280 per year + $7 per plate run on QuantStudio |
DNA/RNA Quantitation
Qubit
Fluorometric concentration assays are generally more accurate than spectrophotometric/UV-Vis absorbance (ie. Nandrop) measurements because they are less affected by common contaminants (ie. Proteins, RNA, or DNA).
This method is recommended for NGS library assessments and for any application that requires higher accuracy in concentration estimates than can be achieved with Nanodrop.
Request Form:
Qubit Request
Equipment Used: Qubit Flex Fluorometer, Qubit® 2.0 Fluorometer
| SERVICE | INTERNAL |
|---|---|
| Qubit analysis | $1.50 per sample, plus $3 for standards |
Droplet Digital PCR (ddPCR)
Droplet Digital PCR technology is a digital PCR method utilizing a water-oil emulsion droplet system. Droplets are formed in a water-oil emulsion to form the partitions that separate the template DNA molecules.
| SERVICE | INTERNAL | EXTERNAL |
|---|---|---|
| One column (8 reactions) | $70 | $80 |
Illumina Sequencing
The Illumina NextSeq 1000 is an NGS sequencing system designed to miniaturize sample and reagent volumes and increase read output. The NextSeq 1000 is capable of many sequencing applications, including targeted metagenomics, whole genome sequencing, transcriptomics, immune repertoire profiling, sequencing of targeted panels, exomes and large panels, circulating tumor DNA, single-cell sequencing, and spatial profiling.
Contact the facility to discuss services and applications.
| SERVICE | INTERNAL | EXTERNAL |
|---|---|---|
| Library prep. & seq. | ||
| Amplicon library preparation and sequencing (250k reads/sample, 2x300 cycles) | $50 | Contact Facility |
| Amplicon library preparation and sequencing (50k reads/sample, 2x300 cycles) | $30 | Contact Facility |
| 16S Profiling (250k reads/sample, 2x300 cycles) | $50 | Contact Facility |
| Library Preparation | ||
| Illumina DNA library preparation | $130 | Contact Facility |
| RNA-Seq Library Prep (PolyA capture) | $115 | Contact Facility |
| RNA-Seq Library Prep (Ribodepletion) | $165 | Contact Facility |
| Library QC | $25 | Contact Facility |
| Sequencing | ||
| NextSeq cartridges | Contact Facility | |
| 300 cycle (1 x 150) P1 | Contact facility | Contact Facility |
| 600 cycle (2 x 300) P1 25M Reads | $1425 per 25M reads | Contact Facility |
| NovaSeq X | Contact Facility (est. installation June 2026) | |
PromethION Services
Plasmid & PCR Amplicon Sequencing
The PromethION 24 is a high-throughput nanopore sequencer capable of natively sequencing both RNA and DNA with rapid turn-around times. The PromethION can load up to 24 flow cells, each capable of multiplexing 96 samples or more. It is also possible to re-use flow-cells, decreasing cost and waste. The PromethION 24 is suitable for long-read applications, including genome assembly, native methylation seq., direct RNA transcript sequencing (including alternate splicing), and metagenomics.
We have phased out Sanger Sequencing in favor of long-read sequencing on the Oxford Nanopore PromethION 24. The facility provides sequencing of DNA fragments up to 25 kilobases in length or more, including whole plasmids and PCR amplicons. Service starts on September 2.
- Sequenced at the University of Guelph: no tax or shipping fees for internal clients
- Plasmid analysis run on Tuesdays and Thursdays with next-day turnaround
- Pricing includes bioinformatic report, plasmid annotation, insert variant calling, and multiplasmid/contamination detection.
- See our detailed sample report
| SERVICE | INTERNAL | ACADEMIA | COMMERCIAL |
|---|---|---|---|
| Whole Plasmid | $19.50 | $19.50 | $22 |
| PCR Amplicon | $19.50 | $19.50 | $22 |
| Batch of 96 Plasmid/Amplicon | $1,632 | $1,632 | $1,900 |
Sequencing Request Form - Use this form to submit DNA templates (e.g. PCR amplicons, Plasmids) for sequencing. Recommended at least 100 ng DNA. Recommended concentration 10 ng/µL in 10 µL in molecular grade water or TE buffer. Concentration should be estimated by fluorometric methods such as Qubit/PicoGreen.
- Fill out all three tabs of the request form (first two tabs are mandatory)
- Signed request form emailed to genomics@uoguelph.ca
- Drop the samples off at room SSC 1401 with facility staff or in the Sample Drop-Off Freezer
Other PromethION Services:
The PromethION is suitable for a range of other services, including native Methylation sequencing (or other modified bases), direct RNA sequencing, 16S profiling (or 18S/ITS/other amplicon metabarcoding), transcriptomics, whole genome sequencing, metagenomics, genome skimming, variant calling, and much more.
| SERVICE | INTERNAL | ACADEMIA | COMMERCIAL |
|---|---|---|---|
| Whole Flow Cell | $1200 | $1200 | $1500 |
| Other applications | Contact us for quote | ||
Real Time PCR
RT-PCR: QuantStudio 7 Pro
This system makes simple and easy to get high-quality real-time PCR results. This automated real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers of all experience levels.
| SERVICE | INTERNAL |
|---|---|
| Full service | $3.00 per reaction (clients provide RNA samples and primers) |
| Self-service | $150 per year for registration and $7 per plate run |
| Consumables | |
| 96 Well qPCR Plate + Seal (pack of 10) | $70 |
| 384 Well qPCR Plate + Seal (pack of 10) | $90 |
| qPCR Seals (pack of 10) | $9 |
| SYBR Green (5 mL tube) | $130 |
Support
| SERVICE | INTERNAL | ACADEMIA | COMMERCIAL |
|---|---|---|---|
| Hourly bioinformatics analysis rate | $100 | $100 | $100 |
| Technical support | $50 | $65 | $80 |
| Consultation | $50 | $65 | $80 |