Mass Spectrometry Services
Small Molecule - Liquid Chromatography Mass Spectrometry
LC-MS can be used to profile and identify small molecules from a mixture. Idealy in water, ACN, MEOH, volatile buffers. Do not use or limit detergents, DMSO,DMF, high salt, non-volatile buffers or stabilizers.
Submit a minimum of 10 µl 0.1-1 mg/ml (approximately)
Submission form for high resolution LC-MS
Submission form for small molecule MW high res chemical formula confirmation
Equipment Used: Agilent LC-UHD Q-Tof
For More Information: Useful information about sample preparation: Thermo LCMS Sample Preparation, Compound identification by MS
Protein ID
Tryptic digest of a simple mixture followed by MS analysis and database search using PEAKS 10 software.
Submission form for Protein ID
Equipment Used: Thermo Easy nLC Orbitrap Exploris 240
For More Information: See Protein ID page
Quantitative Proteomics
Identifying and quantitating proteins from a biological samples using protein digestion, peptide separation, protein identification, peptide intensities and statistical analysis.
Please refer to the Quantitative Proteomics page
Submission Form for Quantitative Proteomics
Equipment Used: Thermo Easy nLC Orbitrap Exploris 240
For More Information: See resources at UWPR Assay development and validation
Protein MW Determination
MW determination for Proteins with desalting column. Samples should be in the range of 0.01-5 mg/ml in volatile buffers with minimal salt and no detergents.
Submission form for Protein MW
Equipment Used: Waters Acquity Synapt G2Si HDMS
For More Information: Best practices for intact protein mass spectrometry
Small Molecule - GC-MS Analysis
Analysis of volatiles and semi-volatiles by gas chromatography.
Sample preparation guidelines.
Equipment Used: Agilent GC-MS, Bruker GC Scion TQ
For More Information: Principles of GC-MS
Small Molecule Quantitation
Quantitation of small molecules by LC-MS requiring a pure standard(s), method development and calibration curve(s). This can also include internal standards. Standards must be provided by the cllient.
Quantitation assays are custom projects that require consultation contact dbrewer@uoguelph.ca
Submission Form for Small Molecule Quantitation
Equipment Used: Bruker EVOQ LC-TQ, Bruker GC Scion TQ
For More Information: Review of method development for small molecule quantitation
Small Molecule - Metabolomics (consult facility)
Refer to Metabolomics (2015) 11:851–860 for experimental design.
Software Used: Agilent Mass Profiler Professional
Submission form for metabolomics
Equipment Used: Agilent LC-UHD Q-Tof
For More Information: Best practices and overview