Mass Spectrometry Services

Small Molecule - Liquid Chromatography Mass Spectrometry

LC-MS can be used to profile and identify small molecules from a mixture. Idealy in water, ACN, MEOH, volatile buffers. Do not use or limit detergents, DMSO,DMF, high salt, non-volatile buffers or stabilizers.

Submit a minimum of 10 µl 0.1-1 mg/ml (approximately)

Submission form for high resolution LC-MS

Submission form for small molecule MW high res chemical formula confirmation

Equipment Used: Agilent LC-UHD Q-Tof

For More Information: Useful information about sample preparation: Thermo LCMS Sample Preparation, Compound identification by MS

Protein ID

Tryptic digest of a simple mixture followed by MS analysis and database search using PEAKS 10 software.

Submission form for Protein ID

Equipment Used: Thermo Easy nLC Orbitrap Exploris 240

For More Information: See Protein ID page

Quantitative Proteomics

Identifying and quantitating proteins from a biological samples using protein digestion, peptide separation, protein identification, peptide intensities and statistical analysis.

Please refer to the Quantitative Proteomics page

Submission Form for Quantitative Proteomics

Equipment Used: Thermo Easy nLC Orbitrap Exploris 240

For More Information: See resources at UWPR Assay development and validation

Protein MW Determination

MW determination for Proteins with desalting column. Samples should be in the range of 0.01-5 mg/ml in volatile buffers with minimal salt and no detergents.

Submission form for Protein MW

Equipment Used: Waters Acquity Synapt G2Si HDMS

For More Information: Best practices for intact protein mass spectrometry

Small Molecule - GC-MS Analysis

Analysis of volatiles and semi-volatiles by gas chromatography.

Sample preparation guidelines.

Sample Submission form.

Equipment Used: Agilent GC-MS, Bruker GC Scion TQ

For More Information: Principles of GC-MS

Small Molecule Quantitation

Quantitation of small molecules by LC-MS requiring a pure standard(s), method development and calibration curve(s). This can also include internal standards. Standards must be provided by the cllient.

Quantitation assays are custom projects that require consultation contact dbrewer@uoguelph.ca

Submission Form for Small Molecule Quantitation

Equipment Used: Bruker EVOQ LC-TQ, Bruker GC Scion TQ

For More Information: Review of method development for small molecule quantitation