Mass Spectrometry Services
Small Molecule - Liquid Chromatography Mass Spectrometry
LC-MS can be used to profile and identify small molecules from a mixture. Idealy in water, ACN, MEOH, volatile buffers. Do not use or limit detergents, DMSO,DMF, high salt, non-volatile buffers or stabilizers.
Submit a minimum of 10 µl 0.1-1 mg/ml (approximately)
Submission form for small molecule MW high res chemical formula confirmation
Equipment Used: Agilent LC-UHD Q-Tof
Identifying and quantitating proteins from a biological samples using protein digestion, peptide separation, protein identification, peptide intensities and statistical analysis.
Please refer to the Quantitative Proteomics page
Equipment Used: Thermo Easy nLC Orbitrap Exploris 240
Protein MW Determination
MW determination for Proteins with desalting column. Samples should be in the range of 0.01-5 mg/ml in volatile buffers with minimal salt and no detergents.
Equipment Used: Waters Acquity Synapt G2Si HDMS
For More Information: Best practices for intact protein mass spectrometry
Small Molecule Quantitation
Quantitation of small molecules by LC-MS requiring a pure standard(s), method development and calibration curve(s). This can also include internal standards. Standards must be provided by the cllient.
Quantitation assays are custom projects that require consultation contact firstname.lastname@example.org
For More Information: Review of method development for small molecule quantitation