Mass Spectrometry Services

Large Molecule - MW Determination

MW determination for Proteins with desalting column.

Submission form for Protein MW

Equipment Used: Agilent LC-UHD Q-Tof

Large Molecule - Protein ID

Tryptic digest followed by MS analysis and database search using PEAKS 8 software.

Submission form for Protein ID

Equipment Used: Agilent LC-UHD Q-Tof

Small Molecule - Gas Chromatography Mass Spectrometry

GC-MS is used to analyze small molecules which are volatile or semi-volatile. Samples can be derivitized to improve volatility.

Useful article about sample preparation from Agilent Technologies.

Submission form for GC-MS

Equipment Used: Agilent GC-MS, Bruker GC Scion TQ

Small Molecule - Liquid Chromatography Mass Spectrometry

LC-MS can be used to profile, identify and quantitate small molecules from a mixture.

Useful article about sample preparation from Agilent Technologies.

Submission form for high resolution LC-MS

Submission form for low resolution LC-MS or infusion

Equipment Used: Agilent LC-UHD Q-Tof, Bruker AmaZon SL LC-MSn

Small Molecule - Metabolomics (consult facility)

Refer to Metabolomics (2015) 11:851–860 for experimental design

Software Used: Agilent Mass Profiler Professional

Submission form for metabolomics

Equipment Used: Agilent LC-UHD Q-Tof