Protein ID

Tryptic digest of a simple mixture followed by MS analysis and database search using PEAKS 10 software.

Submission form for Protein ID

Equipment Used: Thermo Easy nLC Orbitrap Exploris 240

Facility: Mass Spectrometry

Advanced Light Microscopy

Training on and subsequent independent use of advanced light microscopes.

Assisted use of advanced light microscopes.

Facility: Molecular and Cellular Imaging

Agilent TapeStation 4150 Service

The TapeStation system allows for automated electrophoresis of most DNA and RNA samples. Typical applications include quality control (QC) of NGS libraries and RNA extractions (ie. RIN).  This method excels in the determination of fragment length distribution of nucleic acid samples, but is not typically relied on for accurate quantification.  The TapeStation can be used in place of the BioAnalyzer for most applications, but cannot currently be used for small/micro RNA analysis.

Service includes Standard and High Sensitivity assays for RNA, DNA >1kb, and DNA >5kb, as well as a Standard gDNA assay.

Request Form: AAC TapeStation Request Form

 

Equipment Used: Agilent TapeStation 4150

Facility: Genomics

Electron Microscopy

Assisted or independent use of electron microscopes.

Facility: Molecular and Cellular Imaging

Flow Cytometry

Data Acquisition

Cell Sorting

Facility: Molecular and Cellular Imaging

Gene Expression Services

Request Forms:
Digital Droplet PCR Request
Real-Time PCR Registration
Real-Time PCR Request

Equipment Used:
BioRad Qx200 Droplet Digital PCR (ddPCR) System
QuantStudio 7 Pro Real-Time PCR System

Facility: Genomics

Image Processing

  • LAS X - Leica Application Software
  • Volocity
  • Metamorph
  • NIS Elements
  • Zen 2.6 Blue, ZEN Lite
  • Sony Cell Sorter software
  • Gen5 Image+
  • Imod

Facility: Molecular and Cellular Imaging

MALDI-TOF Analysis

Fast mass analysis of proteins, polymers, lipids, DNA/RNA, polysaccharides, organometallic compounds etc. Mass range 500-500 KDa.

Requires co-crystalization of sample with matrix.

Information on matrices can be found Selection Guide of Suitable MALDI Matrix and  here.

Submission form for MALDI-TOF analysis.

 

Equipment Used: Bruker Autoflex maX MALDI-TOF/TOF

Facility: Mass Spectrometry

Next Generation Sequencing

The Illumina NextSeq 1000 is an NGS sequencing system designed to miniaturize sample and reagent volumes and increase read output. The NextSeq 1000 is capable of many sequencing applications, including targeted metagenomics, whole genome sequencing, transcriptomics, immune repertoire profiling, sequencing of targeted panels, exomes and large panels, circulating tumor DNA, single-cell sequencing, and spatial profiling.

Contact the facility to discuss services and applications.

Equipment Used: Illumina NextSeq 1000 Sequencer

Facility: Genomics

NMR External Services

We offer NMR services to industrial, governmental, and academic users across North America. We currently support a wide range of industries, including chemical synthesis, pharma, manufacturing, environmental analysis, medical research, and more.

 

With six spectrometers and over thirty probes we can perform nearly any NMR experiment. Our technical capabilities include:

  • Routine 'small molecule' solution NMR: Proton, Carbon, COSY, HSQC, HMBC, etc.
  • High quality 19F spectra, using a dedicated probe capable of simultaneous 1H decoupling
  • Variable temperature NMR spectra, with sample temperatures ranging from -20 C to 120 C.
  • Solid-state CP/MAS of chemicals, materials, and biomolecules
  • HR-MAS for semi-solid samples
  • Virtually any NMR experiment on any nuclide - please contact us to discuss possibilities!

 

With two PhD-trained NMR spectroscopists on staff, we offer the following services:

  • Guidance in NMR experimental design
  • Data acquisition and processing
  • Analysis and interpretation, including structural elucidation (on a "best-efforts" basis)
  • Basic sample preparation*, including packing/unpacking of solids rotors.
  • An on-campus ‘locker’ for drop-off, pick-up, and safe storage of your samples 
  • Return shipment of your samples by courier for clients outside the Guelph area.  

 

For routine NMR of small molecules, our turnaround time is usually 1 business day, depending on our sample load. We also offer RUSH service for the most time-sensitive samples. Our service includes the raw NMR spectral data for processing on your computers, and we can also provide PDF spectral images for quick viewing of spectra.

Please contact the NMR Centre Manager with any inquiries:

Sameer Al-Abdul-Wahid, PhD
e-mail: s.wahid@uoguelph.ca
phone: 519-824-4120 ext 58914

 

*Samples must be submitted in powder form, as a neat liquid, or already dissolved in an appropriate volume of NMR solvent. We suggest 2 mg/mL for 1H NMR and 20 mg/mL for 13C NMR. If sample limitations are a concern, our high-sensitivity cryoprobes can collect 1H NMR spectra on 0.1 mg/mL samples in a reasonable amount of time. We cannot purify or separate samples: our sample preparation is limited to addition of deuterated solvent and transferring the sample to an NMR tube or sample rotor. We use only Wilmad “Precision” NMR tubes and can supply them at cost if needed.

Facility: NMR Centre