All Services

Agilent BioAnalyzer 2100

Please note, Bioanalyzer Service has been replaced with the TapeStation Service.

 

 

 

Equipment Used: Agilent TapeStation 4150

Facility: Genomics

Advanced Light Microscopy

Training on and subsequent independent use of advanced light micropscopes.

Assisted use of advanced light microscopes.

Facility: Molecular and Cellular Imaging

Agilent TapeStation 4150 Service

The TapeStation system allows for automated electrophoresis of most DNA and RNA samples. Typical applications include quality control (QC) of NGS libraries and RNA extractions (ie. RIN).  This method excels in the determination of fragment length distribution of nucleic acid samples, but is not typically relied on for accurate quantification.  The TapeStation can be used in place of the BioAnalyzer for most applications, but cannot currently be used for small/micro RNA analysis.

Service includes Standard and High Sensitivity assays for RNA, DNA >1kb, and DNA >5kb, as well as a Standard gDNA assay.

Request Form: AAC TapeStation Request Form

 

Equipment Used: Agilent TapeStation 4150

Facility: Genomics

DNA Sequencing

Sample submission considerations.

The facility provides a number of standard sequencing primers for common plasmid vectors.
Custom primers should be provided at a concentration of 5 uM (=5 pmol/uL).

Request Forms:
Individual Sample Submissions
Sanger Sequencing Request - Use this form to submit DNA templates (ie. PCR amplicons, Plasmids, etc.) for sequencing
DIY Sequencing Request - Use this for to submit completed flourescent Sanger products (ie. BigDye reactions) for sequencing

For full and half-plate submissions please contact the facility

Equipment Used: Applied Biosystems 3730 DNA analyzer

Facility: Genomics

Electron Microscopy

Assisted or independent use of electron microscopes.

Facility: Molecular and Cellular Imaging

Fragment Analysis

Image Processing

  • LASAF - Leica Application Software Advanced Fluorescence
  • Volocity
  • Metamorph
  • NIS Elements
  • Zen 2.6 Blue
  • Amira
  • Imod

Facility: Molecular and Cellular Imaging

Large Molecule - MW Determination

MW determination for Proteins with desalting column.

Submission form for Protein MW

Equipment Used: Agilent LC-UHD Q-Tof

Facility: Mass Spectrometry

Large Molecule - Protein ID

Tryptic digest followed by MS analysis and database search using PEAKS 8 software.

Submission form for Protein ID

Equipment Used: Agilent LC-UHD Q-Tof

Facility: Mass Spectrometry