| Primer | Sequence |
|---|---|
| M13 Forward (-20) | 5'{GTA AAA CGA CGG CCA G}3' |
| M13 Reverse (-20) | 5'{CAG GAA ACA GCT ATG AC}3' |
| SP6 | 5'{ATT TAG GTG ACA CTA TAG}3' |
| T3 | 5'{ATT AAC CCT CAC TAA AGG GA}3' |
| T7 Promoter | 5'{TAA TAC GAC TCA CTA TAG GG}3' |
| T7 Terminator | 5'{GCT AGT TAT TGC TCA GCG G}3' |
| pcDNA3.1/BGH Reverse | 5'{TAG AAG GCA CAG TCG AGG}3' |
| 5'-pGEX | 5'{GGG CTG GCA AGC CAC GTT TGG TG}3' |
| 3'-pGEX | 5'{CCG GGA GCT GCA TGT GTC AGA GG}3' |
| TOPO Forward | 5'{GTA AAA CGA CGG CCA G}3' |
| TOPO Reverse | 5'{CAG GAA ACA GCT ATG AC}3' |
| T5 Promoter | 5'{GTG AGC GGA TAA CAA TTT CAC AC}3' |
| pBAD Forward | 5'{ATG CCA TAG CAT TTT TAT CC}3' |
| pBAD Reverse | 5'{GAT TTA ATC TGT ATC AGG}3' |
Concentration Chart
| DNA | Concentration (ng/ul) | Provide (ng) |
|---|---|---|
| dsDNA 1-5 kb | 50 | 25-125 |
| dsDNA 5-10 kb | 100 | 125-250 |
| dsDNA 10-40 kb | 500 | 500 |
| dsDNA > 40 kb | 1000 | 2000 |
| PCR 100-500 bp | 1-10 | 10-40 |
| PCR 500-1000 bp | 20-50 | 40-90 |
| PCR 1000-5000 bp | 50-100 | 90-500 |