All Services

Large Molecule - Protein ID

Tryptic digest followed by MS analysis and database search using PEAKS 8 software.

Submission form for Protein ID

Equipment Used: Agilent LC-UHD Q-Tof

Facility: Mass Spectrometry

Next Generation Sequencing using MiSeq desktop sequencer

  • Metagenomics
  • Small genome sequencing
  • Amplicon sequencing
  • Targeted gene sequencing

Equipment Used: Illumina MiSeq Sequencer

Facility: Genomics

NMR External Services

We are pleased to offer our NMR services to industrial, governmental, and academic users across North America. We currently support a wide range of industries, including chemical synthesis, pharma, manufacturing, environmental analysis, medical research, and more.

With six spectrometers and over thirty probes we can collect almost any NMR experiment. Our technical capabilities include:

  • Routine 'small molecule' solution NMR: Proton, Carbon, COSY, HSQC, HMBC, etc.
  • High quality 19F spectra, using a dedicated probe capable of simultaneous 1H decoupling
  • Variable temperature NMR spectra, with sample temperatures ranging from -20 C to 120 C.
  • Solid-state CP/MAS of chemicals, materials, and biomolecules
  • HR-MAS for semi-solid samples
  • Virtually any NMR experiment on any nuclide - please contact us if there is an experiment you are interested in!

With two PhD-trained NMR spectroscopists on staff, we offer the following services:

  • Guidance in NMR experimental design
  • Data acquisition and processing
  • Analysis and interpretation, including structural elucidation (on a "best-efforts" basis)
  • Basic sample preparation*, including packing/unpacking of solids rotors.
  • An on-campus ‘locker’ for drop-off, pick-up, and safe storage of your samples 
  • Return shipment of your samples by courier for clients outside the Guelph area.  

For routine NMR of small molecules, our turnaround time is usually 1 business day, depending on our sample load. We also offer RUSH service for the most time-sensitive samples. Our service includes the raw NMR spectral data for processing on your computers, and we can also provide PDF spectral images for quick viewing of spectra.

Please contact the NMR Centre Manager with any inquiries:

Sameer Al-Abdul-Wahid, PhD
e-mail: s.wahid@uoguelph.ca
phone: 519-824-4120 ext 58914


*Samples must be submitted in powder form, as a neat liquid, or already dissolved in an appropriate volume of NMR solvent. We suggest 2 mg/mL for 1H NMR and 20 mg/mL for 13C NMR. If sample limitations are a concern, our high-sensitivity cryoprobes can collect 1H NMR spectra on 0.1 mg/mL samples in a reasonable amount of time. We cannot purify or separate samples: our sample preparation is limited to addition of deuterated solvent and transferring the sample to an NMR tube or sample rotor. We use only Wilmad “Precision” NMR tubes and can supply them at cost if needed.

 

 

 

Facility: NMR Centre

Nucleic Acid Fluorometry (Qubit)

Fluorometric concentration assays are generally more accurate than spectrophotometric/UV-Vis absorbance (ie. Nandrop) measurements because they are less affected by common contaminants (ie. Proteins, RNA, or DNA).

This method is recommended NGS library assessments and for any application that requires higher accuracy in concentration estimates than can be acheived with Nanodrop.

 

Request Form:
Qubit Request

 

Equipment Used: Qubit Flex Fluorometer, Qubit® 2.0 Fluorometer

Facility: Genomics

Sample Preparation

  • High Pressure Freezing
  • Plunge Freezing
  • Freeze Substitution
  • Chemical Fixation
  • Embedding
  • Sectioning
  • Staining
  • Immuno-Labeling 
  • Carbon Coating
  • Plasma Cleaning

Facility: Molecular and Cellular Imaging

Small Molecule - Gas Chromatography Mass Spectrometry

GC-MS is used to analyze small molecules which are volatile or semi-volatile. Samples can be derivitized to improve volatility.

Useful article about sample preparation from Agilent Technologies.

Submission form for GC-MS

Equipment Used: Agilent GC-MS, Bruker GC Scion TQ

Facility: Mass Spectrometry

Small Molecule - Liquid Chromatography Mass Spectrometry

LC-MS can be used to profile, identify and quantitate small molecules from a mixture.

Useful article about sample preparation from Agilent Technologies.

Submission form for high resolution LC-MS

Submission form for low resolution LC-MS or infusion

Equipment Used: Agilent LC-UHD Q-Tof

Facility: Mass Spectrometry

Small Molecule - Metabolomics (consult facility)

Refer to Metabolomics (2015) 11:851–860 for experimental design

Software Used: Agilent Mass Profiler Professional

Submission form for metabolomics

Equipment Used: Agilent LC-UHD Q-Tof

Facility: Mass Spectrometry