Quantitative Proteomics

What is quantitative proteomics?

Workflow for protein identification.Proteins are extracted and can be fractionaled,enriched or separated on a gel followed by digestion with protease into small peptides between 8 and 30 aminoacids. These peptides can first be enriched or fractionaled followed by a clean-up and then separated using chromatography and detected by mass spectrometry and fragmented. The data is then analyzed using Peaks 10.0 software and compared to protein databases which leads to protein identification.

 

Quantitative proteomics uses liquid-chromatography (LC)-based separation combined with high-resolution mass spectrometry (MS) to identify proteins from biological samples then quantify relative changes in protein expression in two or more biological states. 

How can you use quantitative proteomics?

LC-MS-based quantitative proteomics is a powerful analytical method that generates large and complex datasets that can be used to examine systematic changes in biological states, monitor specific pathways, or identify individual protein targets. The breadth of options can be difficult to navigate, and proper planning and sample preparation is key to success. Reach out to the Mass Spectrometry Facility (MSF) to learn how quantitative proteomics can benefit your research. The figure MS Quant Proteomics Decision tree below can help you navigate the options.

Workflow for protein identification.Proteins are extracted and can be fractionaled,enriched or separated on a gel followed by digestion with protease into small peptides between 8 and 30 aminoacids. These peptides can first be enriched or fractionaled followed by a clean-up and then separated using chromatography and detected by mass spectrometry and fragmented. The data is then analyzed using Peaks 10.0 software and compared to protein databases which leads to protein identification.

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