Skip to main content
Skip to main content
About AAC
  • Genomics Facility
  • Mass Spectrometry Facility
  • Molecular and Cellular Imaging Facility
  • NMR Centre
  • Phytotron Facility
  • All Equipment
  • Genomics Equipment
  • MS Equipment
  • MCI Equipment
  • NMR Equipment
  • Phytotron Equipment
  • All Services
  • Genomics Services
  • MS Services
  • MCI Services
  • NMR Services
  • General Safety
  • AAC Safety Handbook
SponsorsContact Us

Targeted Quantitative Proteomics MS Methods

You are here

  1. Facilities
  2. Mass Spectrometry Facility
  3. Quantitative Proteomics

Targeted Protein Q MS Methods

These are methods for quantitating a limited number of known proteins with high precision and accuracy in a total protein extract. This method can be particularly useful for low abundance targets.A certain amount of method development/previous data or sample preparation is required regardless of which of these methods are used.

Schematic of PRM Targeted Analysis

Workflow for protein identification.Proteins are extracted and can be fractionaled,enriched or separated on a gel followed by digestion with protease into small peptides between 8 and 30 aminoacids. These peptides can first be enriched or fractionaled followed by a clean-up and then separated using chromatography and detected by mass spectrometry and fragmented. The data is then analyzed using Peaks 10.0 software and compared to protein databases which leads to protein identification.

Discovery-based Quantitative MS methods

Quantitative Proteomics to Label or not to Label

Targeted Quantitative Proteomics MS Methods

MSF LFQ Service: Requirements and Deliverables

Further Reading:

 https://proteomicsresource.washington.edu/protocols05/PRM.php

 

Printer-friendly version

What is the Advanced Analysis Centre?

The Advanced Analysis Centre (AAC) at the University of Guelph is a group of research laboratories housed in the University’s Summerlee Science Complex. The AAC spans an area of over 1000m2 and contains approximately $40 M of research equipment purchased with funds from the Canada Foundation for Innovation, Ontario Research Fund and Natural Sciences and Engineering Research Council of Canada.

more...

Quick Links

  • Genomics Facility
  • Mass Spectrometry Facility
  • Molecular and Cellular Imaging Facility
  • NMR Centre
  • Phytotron Facility

Contact Us

The Advanced Analysis Centre
Summerlee Science Complex
University of Guelph
Guelph, ON N1G 2W1

Phone: (519) 824-4120 ext. 56814
Fax: (519) 767-2044
aac@uoguelph.ca