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BioRad QX200 Droplet Digital PCR (ddPCR) System

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Image of a white and green QX200 AutoDG Droplet Digital PCR System

Droplet digital PCR provides absolute quantification of target DNA or RNA molecules without the use of standard curves and can be run with EvaGreen or hydrolysis probe-based chemistries.

The BioRad QX200 combines water-oil emulsion droplet technology with microfluidics. The AutoDG droplet generator partitions each sample into 20,000 uniform nanoliter-sized droplets and a droplet reader bundled QuantaSoft™ software count the PCR-positive and PCR-negative droplets. ddPCR could be used to:

  • Small fold difference, enable to measure 1.2x difference.
  • Rare targets, low gene expression, low abundance microRNA and single cell analysis
  • Absolute quantification
  • Strong inhibition in samples.

Following image demonstrates the main steps in ddPCR.

.1 Generate droplets, 2. Perform PCR with EvaGreen or hydrolysis probes, 3. Read and analyze results

Request Form: https://www.uoguelph.ca/aac/facilities/genomics/documents/aac-ddpcr-request-form

 

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What is the Advanced Analysis Centre?

The Advanced Analysis Centre (AAC) at the University of Guelph is a group of research laboratories housed in the University’s Summerlee Science Complex. The AAC spans an area of over 1000m2 and contains approximately $40 M of research equipment purchased with funds from the Canada Foundation for Innovation, Ontario Research Fund and Natural Sciences and Engineering Research Council of Canada.

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  • Genomics Facility
  • Mass Spectrometry Facility
  • Molecular and Cellular Imaging Facility
  • NMR Centre
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Contact Us

The Advanced Analysis Centre
Summerlee Science Complex
University of Guelph
Guelph, ON N1G 2W1

Phone: (519) 824-4120 ext. 56814
Fax: (519) 767-2044
aac@uoguelph.ca