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Metabolomics

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  1. Facilities
  2. Mass Spectrometry Facility

Metabolomics is the profiling of small molecules (<1500 Da) of a given sample at a specific time. Metabolites can be endogenous or exogenous, involved in primary or secondary metabolism. Untargeted metabolomics requires matrix and general target dependent sample extraction performed by user in advance. Data analysis is also specialized, MSF has a license for the Agilent Mass Profile Professional software there is also free software available. Access and training on MPP is available for a fee. Helpful references:

https://www.nature.com/articles/nrm.2016.25

https://www.embl.org/groups/metabolomics/protocols-used-for-lc-ms-analysis/

https://link.springer.com/book/10.1007/978-1-59745-244-1

Plant metabolomics

Metabolomics considerations

Metabolomics overview

Please consult MSF on these projects in advance.

 

Metabolomics Decision Tree

Workflow for protein identification.Proteins are extracted and can be fractionaled,enriched or separated on a gel followed by digestion with protease into small peptides between 8 and 30 aminoacids. These peptides can first be enriched or fractionaled followed by a clean-up and then separated using chromatography and detected by mass spectrometry and fragmented. The data is then analyzed using Peaks 10.0 software and compared to protein databases which leads to protein identification.

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What is the Advanced Analysis Centre?

The Advanced Analysis Centre (AAC) at the University of Guelph is a group of research laboratories housed in the University’s Summerlee Science Complex. The AAC spans an area of over 1000m2 and contains approximately $40 M of research equipment purchased with funds from the Canada Foundation for Innovation, Ontario Research Fund and Natural Sciences and Engineering Research Council of Canada.

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  • Genomics Facility
  • Mass Spectrometry Facility
  • Molecular and Cellular Imaging Facility
  • NMR Centre
  • Phytotron Facility

Contact Us

The Advanced Analysis Centre
Summerlee Science Complex
University of Guelph
Guelph, ON N1G 2W1

Phone: (519) 824-4120 ext. 56814
Fax: (519) 767-2044
aac@uoguelph.ca