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  1. Home
  2. Facilities
  3. Mass Spectrometry Facility

Protein ID info

Protein Identification Workflow

Workflow for protein identification.Proteins are extracted and can be fractionaled,enriched or separated on a gel followed by digestion with protease into small peptides between 8 and 30 aminoacids. These peptides can first be enriched or fractionaled followed by a clean-up and then separated using chromatography and detected by mass spectrometry and fragmented. The data is then analyzed using Peaks 10.0 software and compared to protein databases which leads to protein identification.

If you plan to submit any samples for protein identification, it is important that you read the information in this section, to save time and money by avoiding common mistakes.

  • Sample preparation guidelines for protein ID
  • Co-IP: Co-IP, MS compatible Flag tag Co-IP protocol, endogenous Co-IP, 
  • Protocol for solution digestion
  • Protocol for in-gel digestion

Mass Spectrometry Facility

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  • Quantitative Proteomics
    • Discovery-based Quantitative MS methods
    • Quantitative Proteomics to Label or not to Label
    • Targeted Quantitative Proteomics MS Methods
    • MSF LFQ Service: Requirements and Deliverables
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What is the Advanced Analysis Centre?

The Advanced Analysis Centre (AAC) at the University of Guelph is a group of research laboratories housed in the University’s Science Complex. The AAC spans an area of over 1000m2 and contains approximately $30 M of research equipment purchased with funds from the Canada Foundation for Innovation, Ontario Research Fund and Natural Sciences and Engineering Research Council of Canada.

more...

Quick Links

  • Genomics Facility
  • Mass Spectrometry Facility
  • Molecular and Cellular Imaging Facility
  • NMR Centre
  • Phytotron Facility

Contact Us

The Advanced Analysis Centre
Summerlee Science Complex
University of Guelph
Guelph, ON N1G 2W1

Phone: (519) 824-4120 ext. 56814
Fax: (519) 767-2044
aac@uoguelph.ca

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