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Mass Spectrometry

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Selection Guide of Suitable MALDI Matrix

Selection Guide of Suitable MALDI Matrix

MALDI-TOF Analysis

Service Price (CAD$)

MALDI-TOF Sample Preparation and Analysis by MSF

Includes sample preparation with matrix and optimization where necessary, calibrating instrument, acquiring sample data and producing spectrum and peak list report

$35/sample 
 
Training to run samples independantly $50/person
Trained user operation

$20/sample

MALDI-TOF Analysis

Fast mass analysis of proteins, polymers, lipids, DNA/RNA, polysaccharides, organometallic compounds etc. Mass range 500-500 KDa.

Requires co-crystalization of sample with matrix.

Information on matrices can be found Selection Guide of Suitable MALDI Matrix and  here.

Submission form for MALDI-TOF analysis.

 

Equipment Used

Bruker Autoflex maX MALDI-TOF/TOF

Bruker Autoflex maX MALDI-TOF/TOF

MALDI-TOF/TOF MS analysis of:

  • proteins/peptides
  • polymers
  • lipids
  • nucleotides
  • glycans
  • small molecules above 500 Da.

Resolution: up to 26 000

Mass Range: up to 500 000

Mass Accuracy: 2 ppm (internal calibration)

Metabolomics Workflows and Data Integrity

Metabolomics Workflows and Considerations

Solution Protein Digest Protocol

Solution protein digest protocol

BCA Assay Protocol

BCA Assay for Protein Concentration

In-Gel Digest Protocol

In-Gel Digest Protocol

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What is the Advanced Analysis Centre?

The Advanced Analysis Centre (AAC) at the University of Guelph is a group of research laboratories housed in the University’s Summerlee Science Complex. The AAC spans an area of over 1000m2 and contains approximately $40 M of research equipment purchased with funds from the Canada Foundation for Innovation, Ontario Research Fund and Natural Sciences and Engineering Research Council of Canada.

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Quick Links

  • Genomics Facility
  • Mass Spectrometry Facility
  • Molecular and Cellular Imaging Facility
  • NMR Centre
  • Phytotron Facility

Contact Us

The Advanced Analysis Centre
Summerlee Science Complex
University of Guelph
Guelph, ON N1G 2W1

Phone: (519) 824-4120 ext. 56814
Fax: (519) 767-2044
aac@uoguelph.ca