Plasmid & PCR Amplicon Sequencing
The PromethION 24 is a high-throughput nanopore sequencer capable of natively sequencing both RNA and DNA with rapid turn-around times. The PromethION can load up to 24 flow cells, each capable of multiplexing 96 samples or more. It is also possible to re-use flow-cells, decreasing cost and waste. The PromethION 24 is suitable for long-read applications, including genome assembly, native methylation seq., direct RNA transcript sequencing (including alternate splicing), and metagenomics.
We have phased out Sanger Sequencing in favor of long-read sequencing on the Oxford Nanopore PromethION 24. The facility provides sequencing of DNA fragments up to 25 kilobases in length or more, including whole plasmids and PCR amplicons. Service starts on September 2.
- Sequenced at the University of Guelph: no tax or shipping fees for internal clients
- Plasmid analysis run on Tuesdays and Thursdays with next-day turnaround
- Pricing includes bioinformatic report, plasmid annotation, insert variant calling, and multiplasmid/contamination detection.
- See our detailed sample report
| SERVICE | INTERNAL | ACADEMIA | COMMERCIAL |
|---|---|---|---|
| Whole Plasmid | $19.50 | $19.50 | $22 |
| PCR Amplicon | $19.50 | $19.50 | $22 |
| Batch of 96 Plasmid/Amplicon | $1,632 | $1,632 | $1,900 |
Sequencing Request Form - Use this form to submit DNA templates (e.g. PCR amplicons, Plasmids) for sequencing. Recommended at least 100 ng DNA. Recommended concentration 10 ng/µL in 10 µL in molecular grade water or TE buffer. Concentration should be estimated by fluorometric methods such as Qubit/PicoGreen.
- Fill out all three tabs of the request form (first two tabs are mandatory)
- Signed request form emailed to genomics@uoguelph.ca
- Drop the samples off at room SSC 1401 with facility staff or in the Sample Drop-Off Freezer
Other PromethION Services:
The PromethION is suitable for a range of other services, including native Methylation sequencing (or other modified bases), direct RNA sequencing, 16S profiling (or 18S/ITS/other amplicon metabarcoding), transcriptomics, whole genome sequencing, metagenomics, genome skimming, variant calling, and much more.
| SERVICE | INTERNAL | ACADEMIA | COMMERCIAL |
|---|---|---|---|
| Whole Flow Cell | $1200 | $1200 | $1500 |
| Other applications | Contact us for quote | ||
Equipment Used: Oxford Nanopore PromethION 24