Submission form GC-MS 2018
GC-MS
Small Molecule - Metabolomics (consult facility)
MSF Presentations on Metabolomics:
Metabolomics Workflows and Considerations
Metabolomics Worflow and Data Integrity
Refer to Metabolomics (2015) 11:851–860 for experimental design.
Software Used: Agilent Mass Profiler Professional
Mass Spectrometry Buffers
This table shows the MAXIMUM concentration of surfactants, buffers and salts permissible in Maldi and Electrospray ionization techniques. N/A means the technique is not compatible with ANY amount of indicated compound. (Modified from "The Expanding Role of Mass Spectrometry in Biotechnology" by Gary Siuzdak, 2003, pages 84-85)
Sample Preparation
| Service | Price (CAD$) |
|---|---|
| Desalting/clean-up (Ziptip, microcon etc.) | $10/sample |
| In-gel protein digestion | $100/sample |
| In-gel digestion training | $100/person |
| Derivatization for GC-MS | $25/sample (contact facility) |
| Complex protein digestion (lysates, complex mixtures) | $40/sample |
| Digestio |
GC-MS
| Service | Price (CAD$) |
|---|---|
| MSF column | $15/sample |
| MSF column, operated by user | $10/sample (only after training) |
| Researcher column and operated by user (Note, this must be done in a single block of time) | $5/sample (only after training) |
| Method development | $50/sample type (maximum 1 day) |
| Training | $50/person |
Agilent LC-UHD Q-Tof
| Service | Price (CAD$) |
|---|---|
| HPLC-MS-MS | $50/injection |
| High Resolution small molecule | $40/sample |
| Method development (ie metabolomics) | $100/sample (maximum 1 day) |
| Basic data processing and analysis | $20/h (minimum 0.5 hour) |
| Untargeted metabolomics | fees depend on project, contact the Facility |
Protein ID
Tryptic digest of a simple mixture followed by MS analysis and database search using PEAKS 10 software.
Submission form for Protein ID
Equipment Used
Thermo Easy nLC Orbitrap Exploris 240Protein MW Determination
MW determination for Proteins with desalting column. Samples should be in the range of 0.01-5 mg/ml in volatile buffers with minimal salt and no detergents.
Submission form for Protein MW
Equipment Used
Waters Acquity Synapt G2Si HDMSSmall Molecule - Liquid Chromatography Mass Spectrometry
LC-MS can be used to profile and identify small molecules from a mixture. Idealy in water, ACN, MEOH, volatile buffers. Do not use or limit detergents, DMSO,DMF, high salt, non-volatile buffers or stabilizers.
Submit a minimum of 10 µl 0.1-1 mg/ml (approximately)
Submission form for high resolution LC-MS
Submission form for small molecule MW high res chemical formula confirmation