CLSM SP2

  • Filter free spectral detector: a spectrophotometer for each detector channel enables the user to design filters, maximize sensitivity, minimize crosstalk and record emission spectra as required.
  • Microscope: Upright Leica DM RE microscope, mostly manual
  • Objectives: 10x, 20x (Dry); 40x (Oil, Ph); 63x, 100x (Oil, DIC); 40x (Water-Immersion)
  • Fluorescence filters: Leica - A (UV), I3 (blue), N2.1 (green)
  • Laser system: Ar (50 mW): 458 nm, 476 nm, 488 nm, 514 nm; GreenHeNe (1.2 mW): 543 nm; RedHeNe (10 mW): 633 nm
  • Two detection channels for fluorescence as well as transmitted light detection
  • Leica Confocal Software (LCS, version 2.61) for 2D and 3D imaging, Region of Interest (ROI) scanning, and time-lapse imaging, FRAP and FRET applications.