Fluorometric concentration assays are generally more accurate than spectrophotometric/UV-Vis absorbance (ie. Nandrop) measurements because they are less affected by common contaminants (ie. Proteins, RNA, or DNA).
This method is recommended NGS library assessments and for any application that requires higher accuracy in concentration estimates than can be acheived with Nanodrop.
Request Form:
Qubit Request
Equipment Used: Qubit Flex FluorometerQubit® 2.0 Fluorometer
The Advanced Analysis Centre (AAC) at the University of Guelph is a group of research laboratories housed in the University’s Summerlee Science Complex. The AAC spans an area of over 1000m2 and contains approximately $40 M of research equipment purchased with funds from the Canada Foundation for Innovation, Ontario Research Fund and Natural Sciences and Engineering Research Council of Canada.
The Advanced Analysis Centre
Summerlee Science Complex
University of Guelph
Guelph, ON N1G 2W1
Phone: (519) 824-4120 ext. 56814
Fax: (519) 767-2044
aac@uoguelph.ca