NMR Training

Training is a necessary first step before gaining access to the NMR Centre. All users will need to complete either the Basic Training or Metabolomics Training prior to using the NMR equipment. Users who need training in protein NMR or solids NMR should directly email nmr@uoguelph.ca

Basic Training (required for all non-metabolomics users)

In this hands-on session, users will learn about NMR safety, sample preparation, and the NMR software program TopSpin. Users will practice 1D and 2D data collection on the 400 MHz NMR instrument. After Basic Training, users have 30 days to hone their skills before completing a “driver’s test” demonstrating independent and safe operation of the 400 MHz NMR. After passing the “driver’s test”, users can be checked out on the 300 MHz and/or 600 MHz (MacN) NMR, and book time on to run routine experiments on these instruments as needed. Training is offered year-round. Please fill out our Basic Training request form to sign up.

Intermediate Training

In this seminar, users will learn about commonly adjusted experimental parameters (e.g. relaxation delay, sweep width, etc.), and a practical explanation behind the common NMR calibration steps (locking, tuning, shimming, etc.), including a presentation of common mistakes and how to avoid them. Details on the availability of this training session will be available soon.

Advanced Training

In this hands-on session, users will receive custom training specific to their needs (e.g. 600 MHz cryoprobe usage, selective experiments, variable-temperature NMR, advanced 2D experiments, etc.). Both the Intermediate and Advanced Training sessions are required if a user wants to use advanced techniques or start varying parameters. Please email nmr@uoguelph.ca to request Advanced Training. 

Metabolomics Training (required for all metabolomics users)

In this hands-on session, users will learn about NMR safety, metabolomics sample preparation, and be trained in the use of the metabolomics automation interface on the 600 MHz cryoprobe. Metabolomics users do not need to complete any of the other training sessions to collect metabolomics data. Please email nmr@uoguelph.ca to request Metabolomics Training.