Dr. Ross Nazar

Dr. Ross Nazar
Professor Emeritus
Department of Molecular and Cellular Biology
Phone number: 
53004 / 58311
SSC 4457
SSC 4403-4


B.Sc. Toronto
Ph.D. Toronto


Role of RNA processing as a quality control mechanism; studies on the structure, synthesis and regulation of eukaryotic ribosomes in normal and oncogenic tissues; structure and function of the "ribosome assembly chaperone"; regulation of ribosomal protein genes in eukaryotes; PCR-based diagnostics for plant pathogens; regulation of plant defense gene expression.

Selected Publications

S. Nabavi and R.N. Nazar. 2010. Fail-safe termination elements: a common
feature of the eukaryotic genome? FASEB J. 24: 684-688.

Nabavi, S. and Nazar, R.N.  2010, Pac1 endonuclease and Dhp1p 5'-->3'
exonuclease are required for U3 snoRNA termination in Schizosaccharomyces
pombe. FEBS Lett. 584: 3436-3441.

S. Nabavi and R.N. Nazar. 2010. Cleavage-induced termination in U2 snRNA gene expression. Biochem Biophys Res Commun. 12: 461-465.

R.N. Nazar, P. Chen, D. Dean and J. Robb. 2010. DNA chip analysis in diverse organisms with unsequenced genomes. Mol Biotechnol. 44: 8-13.

E.F. Fradin, Z. Zhang, J.C. Juarez Ayala, C.D.M. Castroverde, R.N. Nazar,
J. Robb, C.-M. Liu and B.P.H.J. Thomma. 2009. Genetic Dissection of Verticillium Wilt Resistance Mediated by Tomato Ve1. Plant Physiol. 150: 320-332.

H.O. Shittu, D.C. Castroverde, R.N. Nazar and J. Robb. 2009. Plant-endophyte interplay protects tomato against a virulent Verticillium. Planta 229: 415-426.

H.O. Shittu, A.S. Shakir, R.N. Nazar and J. Robb. 2009. Endophyte-induced
Verticillium protection in tomato is range-restricted. Plant Signaling &
Behavior 4: 160-161.

J. Robb, C.D.M. Castroverde, H.O. Shittu and R.N. Nazar. 2009.  Patterns
of Defence Gene Expression in the Tomato-Verticillium Interaction. Botany
87: 993-1006.

Nabavi S., Nellimarla S. and R.N. Nazar 2008. Post-transcriptional regulation of the U3 small nucleolar RNA. J. Biol. Chem. 283: 21404-10.

S. Nabavi and R.N. Nazar. 2008. Non-polyadenylated RNA polymerase II termination is induced by transcript cleavage. J. Biol. Chem. 283: 13601-10.

K. Spasov and R.N. Nazar. 2008. RAC protein induces enzymatic access to the maturing 3'-end of the 25S rRNA in Schizosaccharomyces pombe. Biochem. Biophys. Res. Commun. 368: 374-378.

Chang A, Lim MH, Lee SW, Robb J, Nazar RN. 2008. Tomato pal gene family: Highly redundant but strongly underutilized. J. Biol. Chem., first online.

Nabavi S, Nazar RN. 2008. U3 snoRNA promoter reflects the RNA's function in ribosome biogenesis. Curr. Genet. 54: 175-84.

H.O. Shittu, C.D.M. Castroverde, R.N. Nazar and J. Robb. 2008. Plant-endophyte interplay protects tomato against a virulent Verticillium. Planta., first online.

Robb J., B. Lee and R.N. Nazar. 2007. Gene suppression in a tolerant tomato-vascular pathogen interaction. Planta. 226: 299–309.

R.N. Nazar and J. Robb. Borrowing from biology. 2007. Science. 318: 565.

Abeyrathne PD, Nazar RN. Parallels in rRNA processing: conserved features in the processing of the internal transcribed spacer 1 in the pre-rRNA from Schizosaccharomyces pombe. Biochemistry. 2005 Dec 27;44(51):16977-87.

Nabavi S, Nazar RN. Simplified one-tube "megaprimer" polymerase chain reaction mutagenesis. Anal Biochem. 2005 Oct 15;345(2):346-8.

Nazar RN. Ribosomal RNA processing and ribosome biogenesis in eukaryotes. IUBMB Life. 2004, 6(8):457-65.

Yi JY, Seo HW, Yang MS, Robb EJ, Nazar RN, Lee SW Plant defense gene promoter enhances the reliability of shiva-1 gene-induced resistance to soft rot disease in potato. Planta. 2004 Nov;220(1):165-71.

Ivakine E, Spasov K, Frendewey D, Nazar RN. (2003) Functional significance of intermediate cleavages in the 3'ETS of the pre-rRNA from Schizosaccharomyces pombe. Nucleic Acids Res. 31(24): 7110-6.

Lee Y, Nazar RN. (2003) Terminal structure mediates 5 S rRNA stability and integration during ribosome biogenesis. J Biol Chem. 278(9): 6635-41.

Volossiouk T, Robb EJ Nazar RN. (2003) Avoiding false positives in PCR-based identification methods for non-sterile plant pathogens. Can. J. Plant Pathol. 25: 192-19.

Nazar RN. (2003) Ribosome Biogenesis in Yeast: rRNA Processing and Quality Control. In: Applied Mycology & Biotechnology, Volume 3: Fungal Genomics and Bioinformatics (Arora, D.K. and Khachatourians, G.G., eds) pp. 161-185, Elsevier Inc. Science & Technology/Academic Press, San Diego, CA.

Song X, Nazar RN. (2002) Modification of rRNA as a 'quality control mechanism' in ribosome biogenesis.
FEBS Lett. 523(1-3): 182-6.

Abeyrathne PD, Lalev AI, Nazar RN. (2002) RAC protein binding site in the internal transcribed spacer 2 of pre-rRNA transcripts from Schizosaccharomyces pombe. J Biol Chem. 277(24): 21291-9.

Spasov K, Perdomo LI, Evakine E, Nazar RN. (2002) RAC protein directs the complete removal of the 3' external transcribed spacer by the Pac1 nuclease. Mol Cell. 9(2): 433-7.

Nazar RN, Abeyrathne PD, Intine RV. (2002) Polymerase chain reaction-mediated mutagenesis in sequences resistant to homogeneous amplification. In: Methods in Molecular Biology: In Vitro Mutagenesis Protocols, 2nd Edition (Braman, J., ed) 282: 117-26.

Lalev AI, Nazar RN. (2001) A chaperone for ribosome maturation. J Biol Chem. 276: 16655-9.

Abeyrathne PD, Nazar RN. (2000) Plasmid-enhanced strategy for PCR-mediated mutagenesis with difficult DNA templates. Biotechniques 29: 1172-4, 1176. No abstract available.

Lalev AI, Abeyrathne PD, Nazar RN. (2000) Ribosomal RNA maturation in Schizosaccharomyces pombe is dependent on a large ribonucleoprotein complex of the internal transcribed spacer 1. J Mol Biol. 302: 65-77.

Leal-Klevezas DS, Martinez-Soriano JP, Nazar RN. 2000. Cotranscription of 5S rRNA-tRNA(Arg)(ACG) from Brassica napus chloroplasts and processing of their intergenic spacer. Gene 253: 303-11.

Lalev AI, Nazar RN. (1999) Structural equivalence in the transcribed spacers of pre-rRNA transcripts in
Schizosaccharomyces pombe. Nucleic Acids Res. 27: 3071-8.

Shwed PS, Nazar RN. (1999) Terminator element mutations affect both the efficiency and position of RNA polymerase I termination in Schizosaccharomyces pombe. Nucleic Acids Res. 27: 2883-8.

Intine RV, Good L, Nazar RN. (1999) Essential structural features in the Schizosaccharomyces pombe pre-rRNA 5' external transcribed spacer. J Mol Biol, 286: 695-708.

Lalev AI, Nazar RN. (1998) Conserved core structure in the internal transcribed spacer 1 of the Schizosaccharomyces pombe precursor ribosomal RNA. J Mol Bio, 284: 1341-1351.

Intine RV, Nazar RN. (1998) PCR-mediated mutagenesis in sequences recalcitrant to homogeneous amplification. Biotechniques, 25: 364-366.

Heinz R, Lee SW, Saparno A, Nazar RN. Robb J. (1998) Cyclical systemic colonization in Verticillium-infected tomato. Physiological and Molecular Plant Pathology 52: 385-396.

Graduate Students

C.D. Castroverde - M.Sc.*
W. Van Loggerenberg - M.Sc.
A. Mohamed - M.Sc.**

* co-supervised with E.J. Robb
** co-supervised with G. Bag