The Guelph Wastewater Facility (GWF) is a bench and pilot-scale facility constructed adjacent to the City of Guelph’s municipal Wastewater Treatment Plant (WWTP). The GWF, owned and operated by the University of Guelph and supported by Ontario Water Consortium (OWC), has five pilot-scale stations, an Ecotoxicology Lab, and two basic analytical labs. The users have access to various process streams, including raw wastewater, primary effluent, secondary effluent, tertiary effluent, raw sludge, waste activated sludge (WAS) and thickened WAS. The facility also includes two complementary, state-of-the-art analytical labs located in the University of Guelph. Analytical equipment include - GC/MS, TOC, Respirometer, Real-Time PCR, Refrigerated Centrifuge, Incubator shaker, Autoclave, and a Digital Imaging Fluorescence Microscope.