Dr. Jennifer Geddes-McAlister

Dr. Jennifer Geddes-McAlister
Assistant Professor
Department of Molecular and Cellular Biology
Email: 
jgeddesm@uoguelph.ca
Phone number: 
52129
Office: 
SSC 4457
Lab: 
SSC 4403

My keen interest in scientific research and academics began during my undergraduate degree at the University of Lethbridge, where I investigated the impact of fungal pathogens on agricultural development and performed my first proteomics experiments. This amazing experience led to a PhD in the laboratory of Dr. Jim Kronstad at UBC, where I applied quantitative proteomics to comprehensively profile the cellular proteome and secretome of the fungal pathogen, Cryptococcus neoformans. My fascination with mass spectrometry-based proteomics and pathogenesis provided the foundation for a postdoctoral fellowship supported by NSERC and the Alexander von Humboldt Foundation in the laboratory of Prof. Dr. Matthias Mann at the Max Planck Institute of Biochemistry in Munich, Germany. During my postdoc, I combined my expertise in microbiology and proteomics with experimental systems immunology to profile the interplay between Salmonella and its host during infection. At the University of Guelph, my research group will blend cutting-edge proteomics technologies with a systems biology perspective to explore host-pathogen interactions.

  • BSc (co-op) – University of Lethbridge, Biological Sciences
  • MSc – University of Lethbridge, Biological Sciences
  • PhD – University of British Columbia, Microbiology and Immunology
  • Postdoctoral Fellow – Max Planck Institute of Biochemistry, Proteomics and Signal Transduction

We are interested in characterizing the mechanisms of pathogenesis, adaptation, and survival in fungal and bacterial microbes from a systems biology perspective through mass spectrometry-based quantitative proteomics. Specifically, research in the lab centres around the following areas:

  1. Differential patterns of pathogenic regulation between microbial species;
  2. Systems biology to elucidate microbial proteome dynamics and interactions;
  3. Mechanistic characterization of pathogenic proteins; and
  4. Mass spectrometry-based proteomics for drug discovery and repurposing.

Our long-term goals emphasize the utilization of mass spectrometry-based proteomics to fundamentally understand differential microbial adaptation and survival strategies and to integrate a novel systems biology platform for investigation of diverse biological systems, defining functional mechanisms of cellular regulation and pathogenesis.

Interested students and post-doctoral fellows are encouraged to contact me to learn more about new and exciting opportunities.

2020

(*denotes corresponding authorship)

  • Prudhomme, N., Pastora, R., McLean, M.D., Cossar, D., Geddes-McAlister, J.* (In Review) Temporal proteomic profiling of Agrobacterium tumefaciens exposure to agroinfiltration media influences cellular remodeling and may promote enhanced adaptability for molecular farming.
  • Prudhomme, N., Gianetto-Hill, C., Pastora, R., Cheung, W.-F., Allen-Vercoe, E., McLean, M.D., Cossar, D., Geddes-McAlister, J.* (In Review) Distinct proteome profiles of Agrobacterium tumefaciens grown in bioreactors suggests bacterial adaptability and preparedness for plant transformation.
  • Petrie, L.E., Leonard, A.C., Goncheva, M.I., Murphy, J., Thompson, L., Geddes-McAlister, J., Heinrichs, D.E., Cox, G. (In Review) The Staphylococcus aureus host cell genetic adhesion network.
  • Sukumaran, A., Geddes-McAlister, J.* (Accepted - In Revision) Zinc limitation in Klebsiella pneumoniae profiled by quantitative proteomics influences transcriptional regulation and cation transporter-associated capsule production.
  • Bermas, A., Geddes-McAlister, J.* (Accepted - In Revision) Combatting the evolution of antifungal resistance in Cryptococcus neoformans. (Invited submission)
  • Muselius, B.1, Sukumaran, A.1, Yeung, J., Geddes-McAlister, J.* (2020) Iron limitation in Klebsiella pneumoniae defines new roles for Lon protease in homeostasis and degradation by quantitative proteomics. Front Microbiol1Authors contributed equally.
  • Anderson, E.M., Sychantha, D., Brewer, D., Clarke, A.J., Geddes-McAlister, J.*, Khursigara, C.M.* (2020) Peptidoglycomics: Examining compositional changes in peptidoglycan between biofilm- and planktonic-derived Pseudomonas aeruginosa. J Biol Chem.
  • Yeung, J., Gadjeva, M., Geddes-McAlister, J.* (2020) Label-free quantitiative proteomics distinguishes general -and site-specific host responses to Pseudomonas aeruginosa infection at the ocular surface. Proteomics.

2019

  • Geddes-McAlister, J., Kugadas, A., Gadjeva, M. (2019) Tasked with a challenging objective: why do neutrophils fail to battle Pseudomonas aeruginosa biofilms? Pathogens. (Invited submission)
  • Ball, B., Geddes-McAlister, J.* (2019) Quantitative proteomic profiling of Cryptococcus neoformans. Curr Protoc Microbiol. (Invited submission & cover image)
  • Sukumaran, A., Coish, J., Yeung, J., Muselius, B., Gadjeva, M., MacNeil, A.J., Geddes-McAlister, J.* (2019). Decoding communication patterns of the innate immune system by quantitative proteomics. J Leukocyte Biol.
  • Geddes-McAlister, J.*, Gadjeva, M. (2019). Mass spectrometry-based quantitative proteomics of murine-derived polymorphonuclear neutrophils. Current Protocols in Immunology.
  • Ball, B., Bermas, A., Carruthers-Lay, D., Geddes-McAlister, J.* (2019) Mass Spectrometry-Based Proteomics of Fungal Pathogenesis, Host–Fungal Interactions, and Antifungal Development. J Fungi.
  • Rea, K.A., Casaretoo, J.A., Al-Abdul-Wahid, M.S., Sukumaran, A., Geddes-McAlister, J., Rothstein, S.J., Akhtar, T.A. (2019). Biosynthesis of cannflavins A and B from Cannabis sativa L. Phytochemistry.
  • Kugadas A., Geddes-McAlister, J., Guy, E., DiGiandomenico, A., Sykes, D.B., Mansour, M.K., Mirchev, R., Gadjeva, M. (2019). Employing enzymatic treatment options for management of ocular biofilm-based infections. J Leukocyte Biol.

2017 & 2018

  • Geddes-McAlister, J., Shapiro, R.S. (2018) New pathogens, new tricks: emerging, drug-resistant fungal pathogens and future prospects for antifungal therapeutics. Antimicrobial Therapeutics Reviews, Annals of the New York Academy of Sciences.
  • Kugadas A., Wright Q., Geddes-McAlister, J., Gadjeva, M. (2017) Role of microbiota in strengthening ocular mucosal barrier function through secretory IgA. Investigative Ophthalmology & Visual Science.

2015 & 2016

  • Geddes, J.M.H., Caza, M., Croll, D., Stoynov, N., Foster, L.J., Kronstad, J.W. (2016) The ubiquitin-proteasome pathway in Cryptococcus neoformans influences capsule production, is regulated by protein kinase A, and is an antifungal target. MBio.
  • Geddes, J.M.H., Croll, D., Caza, M., Stoynov, N., Foster, L.J., Kronstad, J.W. (2015) Secretome profiling of Cryptococcus neoformans reveals regulation of a subset of virulence-associated proteins and potential biomarkers by Protein Kinase A. BMC Microbiol.

2012 & earlier

  • Kronstad, J.W., Saikia, S., Nielson, E.D., Kretschmer, M., Jung, W., Hu, G., Geddes, J.M.H., Griffiths, E.J., Choi, J., Cadieux, B., Caza, M., Attarian, R. (2012) Adaptation of Cryptococcus neoformans to mammalian hosts: integrated regulation of metabolism and virulence. Eukaryotic Cell.
  • Kronstad, J.W., Attarian, R., Cadieux, B., Choi, J., D’Souza, C.A., Geddes, J.M.H., Griffiths, E.J., Hu, G., Jung, W., Kretschmer, M., Saikia, S., Wang, J. (2011) Expanding fungal pathogenesis: Cryptococcus breaks out of the opportunistic box. Nature Reviews Microbiology.
  • Geddes, J.M.H., Eudes, F., Laroche, A., Selinger, L. B. (2008) Differential expression of proteins in response to the interaction between the pathogen Fusarium graminearum and its host, Hordeum vulgare. Proteomics.
  • Geddes, J.M.H., Eudes, F., Tucker, J. R., Legge, W. G., Selinger, L.B. (2008) Evaluation of inoculation methods on Fusarium graminearum infection and deoxynivalenol production in barley. Canadian Journal of Plant Pathology.

Graduate Students:

  • Arjun Sukumaran (PhD)
  • Brianna Ball (MSc)
  • Arianne Bermas (MSc)
  • Nicholas Prudhomme (MSc)
  • Lilianne Gee (MSc) (Co-supervised with Dr. Jonathan Newman, Laurier University)
  • Jacqueline Thomson (MSc) (Co-supervised with Dr. Jonathan Newman, Laurier University)

Undergraduate Project:

  • Benjamin Muselius
  • Rebecca Stevens-Green (Co-supervised with Dr. Rebecca Shapiro)
  • Elizabeth Woroszchuk
  • Jason Yeung

Undergraduate Students:

  • Edison Zheng
  • Taylor Ross
  • Nadolina Brajuka

Previous Members:

  • Duncan Carruthers-Lay
  • Ammar Afaq

MICRO*3230 Immunology