Dr. Jennifer Geddes-McAlister

Dr. Jennifer Geddes-McAlister
Assistant Professor
Department of Molecular and Cellular Biology
Email: 
jgeddesm@uoguelph.ca
Phone number: 
52129
Office: 
SSC 4457
Lab: 
SSC 4403

My keen interest in scientific research and academics began during my undergraduate degree at the University of Lethbridge, where I investigated the impact of fungal pathogens on agricultural development and performed my first proteomics experiments. This amazing experience led to a PhD in the laboratory of Dr. Jim Kronstad at UBC, where I applied quantitative proteomics to comprehensively profile the cellular proteome and secretome of the fungal pathogen, Cryptococcus neoformans. My fascination with mass spectrometry-based proteomics and pathogenesis provided the foundation for a postdoctoral fellowship supported by NSERC and the Alexander von Humboldt Foundation in the laboratory of Prof. Dr. Matthias Mann at the Max Planck Institute of Biochemistry in Munich, Germany. During my postdoc, I combined my expertise in microbiology and proteomics with experimental systems immunology to profile the interplay between Salmonella and its host during infection. At the University of Guelph, my research group will blend cutting-edge proteomics technologies with a systems biology perspective to explore host-pathogen interactions.

  • BSc (co-op) – University of Lethbridge, Biological Sciences
  • MSc – University of Lethbridge, Biological Sciences
  • PhD – University of British Columbia, Microbiology and Immunology
  • Postdoctoral Fellow – Max Planck Institute of Biochemistry, Proteomics and Signal Transduction

We are interested in characterizing the mechanisms of pathogenesis, adaptation, and survival in fungal and bacterial microbes from a systems biology perspective through mass spectrometry-based quantitative proteomics. Specifically, research in the lab centres around the following areas:

  1. Differential patterns of pathogenic regulation between microbial species;
  2. Systems biology to elucidate microbial proteome dynamics and interactions;
  3. Mechanistic characterization of pathogenic proteins; and
  4. Mass spectrometry-based proteomics for drug discovery and repurposing.

Our long-term goals emphasize the utilization of mass spectrometry-based proteomics to fundamentally understand differential microbial adaptation and survival strategies and to integrate a novel systems biology platform for investigation of diverse biological systems, defining functional mechanisms of cellular regulation and pathogenesis.

Interested students and post-doctoral fellows are encouraged to contact me to learn more about new and exciting opportunities.

2019

  • Muselius, B., Yeung, J., Sukumaran, A., Geddes-McAlister, J.* (In Review) Iron limitation in Klebsiella pneumoniae defines new roles for Lon protease in homeostasis and degradation by quantitative proteomics.
  • Yeung, J., Gadjeva, M., Geddes-McAlister, J.* (In Review) Quantitative proteomics distinguishes general- and site-specific host responses to Pseudomonas aeruginosa infection at the ocular surface
  • Geddes-McAlister, J., Meissner, F., Mann, M., Bantscheff, M. (In Revision). The emerging role of mass spectrometry-based proteomics in drug discovery.
  • Anderson, E.M., Sychantha, D., Brewer, D., Clarke, A.J., Geddes-McAlister, J.*, Khursigara, C.M.* (In Revision). Peptidoglycomics: Examining compositional changes in peptidoglycan between biofilm- and planktonic-derived Pseudomonas aeruginosa.
  • Sukumaran, A., Coish, J., Yeung, J., Muselius, B., Gadjeva, M., MacNeil, A.J., Geddes-McAlister, J.* (2019). Decoding communication patterns of the innate immune system by quantitative proteomics. DOI:10.1002/JLB.2RI0919-302R
  • Geddes-McAlister, J.*, Gadjeva, M. (2019). Mass spectrometry-based quantitative proteomics of murine-derived polymorphonuclear neutrophils. Current Protocols in Immunology. Doi.org/10.1002/cpim.87
  • Ball, B., Bermas, A., Carruthers-Lay, D., Geddes-McAlister, J.* (2019) Mass Spectrometry-Based Proteomics of Fungal Pathogenesis, Host–Fungal Interactions, and Antifungal Development. J Fungi. DOI: 10.3390/jof5020052.
  • Rea, K.A., Casaretoo, J.A., Al-Abdul-Wahid, M.S., Sukumaran, A., Geddes-McAlister, J., Rothstein, S.J., Akhtar, T.A. (2019). Biosynthesis of cannflavins A and B from Cannabis sativa L. Phytochemistry. 164: 162-171. doi.org/10.1016/j.phytochem.2019.05.009
  • Kugadas A., Geddes-McAlister, J., Guy, E., DiGiandomenico, A., Sykes, D.B., Mansour, M.K., Mirchev, R., Gadjeva, M. (2019). Employing enzymatic treatment options for management of ocular biofilm-based infections. J Leukocyte Biol. 1-12, DOI: 10.1002/JLB.4HI0918-364RR.

2017 & 2018

  • Geddes-McAlister, J., Shapiro, R.S. (2018) New pathogens, new tricks: emerging, drug-resistant fungal pathogens and future prospects for antifungal therapeutics. Antimicrobial Therapeutics Reviews, Annals of the New York Academy of Sciences. DOI:10.1111/nyas.13739.
  • Kugadas A., Wright Q., Geddes-McAlister, J., Gadjeva, M. (2017) Role of microbiota in strengthening ocular mucosal barrier function through secretory IgA. Investigative Ophthalmology & Visual Science. 58: 4593-4600. Doi: 10.1167/iovs.17-22119.

2015 & 2016

  • Geddes, J.M.H., Caza, M., Croll, D., Stoynov, N., Foster, L.J., Kronstad, J.W. (2016) The ubiquitin-proteasome pathway in Cryptococcus neoformans influences capsule production, is regulated by protein kinase A, and is an antifungal target. MBio. 7: e01862-15. DOI: 10.1128/mBio.01862-15
  • Geddes, J.M.H., Croll, D., Caza, M., Stoynov, N., Foster, L.J., Kronstad, J.W. (2015) Secretome profiling of Cryptococcus neoformans reveals regulation of a subset of virulence-associated proteins and potential biomarkers by Protein Kinase A. BMC Microbiol. 15: 206. DOI 10.1186/s12866-015-0532-3.

2012 & earlier

  • Kronstad, J.W., Saikia, S., Nielson, E.D., Kretschmer, M., Jung, W., Hu, G., Geddes, J.M.H., Griffiths, E.J., Choi, J., Cadieux, B., Caza, M., Attarian, R. (2012) Adaptation of Cryptococcus neoformans to mammalian hosts: integrated regulation of metabolism and virulence. Eukaryotic Cell. 11: 109-118. Doi: 10.1128/EC.05273-11
  • Kronstad, J.W., Attarian, R., Cadieux, B., Choi, J., D’Souza, C.A., Geddes, J.M.H., Griffiths, E.J., Hu, G., Jung, W., Kretschmer, M., Saikia, S., Wang, J. (2011) Expanding fungal pathogenesis: Cryptococcus breaks out of the opportunistic box. Nature Reviews Microbiology. 9: 193-203. DOI: 10.1038/nrmicro2522
  • Geddes, J.M.H., Eudes, F., Laroche, A., Selinger, L. B. (2008) Differential expression of proteins in response to the interaction between the pathogen Fusarium graminearum and its host, Hordeum vulgare. Proteomics. 8: 545-554. DOI: 10.1002/pmic.200700115
  • Geddes, J.M.H., Eudes, F., Tucker, J. R., Legge, W. G., Selinger, L.B. (2008) Evaluation of inoculation methods on Fusarium graminearum infection and deoxynivalenol production in barley. Canadian Journal of Plant Pathology. 30: 66-73. doi.org/10.1080/07060660809507497

 

Graduate Students:

  • Arjun Sukumaran (PhD)
  • Brianna Ball (MSc)
  • Arianne Bermas (MSc)
  • Nicholas Prudhomme (MSc)
  • Lilianne Gee (MSc) (Co-supervised with Dr. Jonathan Newman, Laurier University)
  • Jacqueline Thomson (MSc) (Co-supervised with Dr. Jonathan Newman, Laurier University)

Undergraduate Project:

  • Benjamin Muselius
  • Rebecca Stevens-Green (Co-supervised with Dr. Rebecca Shapiro)
  • Elizabeth Woroszchuk
  • Jason Yeung

Undergraduate Students:

  • Edison Zheng
  • Taylor Ross
  • Nadolina Brajuka

Previous Members:

  • Duncan Carruthers-Lay
  • Ammar Afaq

MICRO*3230 Immunology