Dr. Jennifer Geddes-McAlister

Dr. Jennifer Geddes-McAlister
Assistant Professor
Department of Molecular and Cellular Biology
Email: 
jgeddesm@uoguelph.ca
Phone number: 
52129
Office: 
SSC 4457
Lab: 
SSC 4405/4406

My keen interest in scientific research and academics began during my undergraduate degree at the University of Lethbridge, where I investigated the impact of fungal pathogens on agricultural development and performed my first proteomics experiments. This amazing experience led to a PhD in the laboratory of Dr. Jim Kronstad at UBC, where I applied quantitative proteomics to comprehensively profile the cellular proteome and secretome of the fungal pathogen, Cryptococcus neoformans. My fascination with mass spectrometry-based proteomics and pathogenesis provided the foundation for a postdoctoral fellowship supported by NSERC and the Alexander von Humboldt Foundation in the laboratory of Prof. Dr. Matthias Mann at the Max Planck Institute of Biochemistry in Munich, Germany. During my postdoc, I combined my expertise in microbiology and proteomics with experimental systems immunology to profile the interplay between Salmonella and its host during infection. At the University of Guelph, my research group will blend cutting-edge proteomics technologies with a systems biology perspective to explore host-pathogen interactions.

  • BSc (co-op) – University of Lethbridge, Biological Sciences
  • MSc – University of Lethbridge, Biological Sciences
  • PhD – University of British Columbia, Microbiology and Immunology
  • Postdoctoral Fellow – Max Planck Institute of Biochemistry, Proteomics and Signal Transduction

We are interested in characterizing the mechanisms of pathogenesis, adaptation, and survival in fungal and bacterial microbes from a systems biology perspective through mass spectrometry-based quantitative proteomics. Specifically, research in the lab centres around the following areas:

  1. Differential patterns of pathogenic regulation between microbial species;
  2. Systems biology to elucidate microbial proteome dynamics and interactions;
  3. Mechanistic characterization of pathogenic proteins; and
  4. Mass spectrometry-based proteomics for drug discovery and repurposing.

Our long-term goals emphasize the utilization of mass spectrometry-based proteomics to fundamentally understand differential microbial adaptation and survival strategies and to integrate a novel systems biology platform for investigation of diverse biological systems, defining functional mechanisms of cellular regulation and pathogenesis.

Interested students and post-doctoral fellows are encouraged to contact me to learn more about new and exciting opportunities.

2020

(*denotes corresponding authorship)

  • Meissner, F., Geddes-McAlister, J., Mann, M., Bantscheff, M. (In review) The emerging role of mass spectrometry-based proteomics in drug discovery. Nature Review Drug Discovery. (Invited submission)
  • Stevens-Green, R.1, Johal, D.1, Geddes-McAlister, J.* (In review) Proteomics of Fusarium head blight: diverse technological applications provide new insights into histroric problems. Proteomics. (Invited submission). 1Authors contributed equally
  • Geddes-McAlister, J.*, Sukumaran, A., Patchett, A., Hager, H.A., Dale, J.C.M., Roloson, J.L., Prudhomme, N., Bolton, K., Muselius, B., Powers, J.A. (In review) Examining the impacts of CO2 conecentration and genetic imcompatibility on pereniial ryegrass-Epichloee festucae var lolii interactions.
  • Sukumaran, A., Geddes-McAlister, J.* (Accepted - In Revision) Zinc limitation in Klebsiella pneumoniae profiled by quantitative proteomics influences transcriptional regulation and cation transporter-associated capsule production. BMC Microbiol.
  • Sukumaran, A., Woroszchuk, E., Ross, T., Geddes-McAlister, J.* (Accepted - in revision) Proteomics of host-bacterial interactions: new insights from dual perspectives. Can. J. Microbiol. (Invited submission)
  • Ball, B., Sukumaran, A., Geddes-McAlister, J.* (In Press) Label-Free Quantitative Proteomics Workflow for Discover-Driven Host-Pathogen Interactions. J Visual Experiments. (Invited submission).
  • Ball., B., Langille, M., Geddes-McAlister, J.* (In Press) Fun(gi)OMICS: advanced and diverse technologies to explore emerging fungal pathogens. MBio. (Invited submission)
  • Bermas, A. and Geddes-McAlister, J.* (In Press) Experimental evolution of antifungal resistance in Cryptocossus neoformans. Curr Prot Microbiol. (Invited submission)
  • Prudhomme, N., Gianetto-Hill, C., Pastora, R., Cheung, W.-F., Allen-Vercoe, E., McLean, M.D., Cossar, D., Geddes-McAlister, J.* (2020) Quantitative proteomic profiling of shake flask bioreactor growth reveals distinct responses of Agrobacerium tumefaciens for perparation in molecular pharming. Can. J. Microbiol.
  • Yeung, J., Lamb., J., Krieger, J., Gadjeva, M., Geddes-McAlister, J.* (2020) Quantitative proteomic profiling of murine ocular tissue and the extracellular environment. Curr Prot Mouse Biol. (Invited submission)
  • Prudhomme, N., Pastora, R., Muselius, B., McLean, M.D., Cossar, D., Geddes-McAlister, J.* (2020) Exposure of Agrobacterium tumefaciens to agroinfiltration medum demonstrates cellular remodeling and may promote enhanced adaptability for molecular pharming. Can. J. Microbiol.
  • Geddes-McAlister, J.* (2020) Pathogenesis of Fungal and Bacterial Microbes. Pathogens. (Editorial; invited submission)
  • Horianopolous, L., Gluck-Thaler, L., Benoit Gelber, I., Cowen, L., Geddes-McAlister, J., Landry, C., Schwartz, I., Scott, J., Sellam, A., Shappard, D., Spribille, T., Subramaniam, R., Walker, A., Harris, S., Sahpiro, R., Gerstain, A. (2020) The Candian Fungal Research Network: current challenges and future opportunities. Can. J. Microbiol.
  • Bermas, A., Geddes-McAlister, J.* (2020) Combatting the evolution of antifungal resistance in Cryptococcus neoformans. Mol Micro. (Invited submission)
  • Muselius, B.1, Sukumaran, A.1, Yeung, J., Geddes-McAlister, J.* (2020) Iron limitation in Klebsiella pneumoniae defines new roles for Lon protease in homeostasis and degradation by quantitative proteomics. Front Microbiol1Authors contributed equally.
  • Anderson, E.M., Sychantha, D., Brewer, D., Clarke, A.J., Geddes-McAlister, J.*, Khursigara, C.M.* (2020) Peptidoglycomics: Examining compositional changes in peptidoglycan between biofilm- and planktonic-derived Pseudomonas aeruginosa. J Biol Chem.
  • Yeung, J., Gadjeva, M., Geddes-McAlister, J.* (2020) Label-free quantitiative proteomics distinguishes general -and site-specific host responses to Pseudomonas aeruginosa infection at the ocular surface. Proteomics.

2019

  • Geddes-McAlister, J., Kugadas, A., Gadjeva, M. (2019) Tasked with a challenging objective: why do neutrophils fail to battle Pseudomonas aeruginosa biofilms? Pathogens. (Invited submission)
  • Ball, B., Geddes-McAlister, J.* (2019) Quantitative proteomic profiling of Cryptococcus neoformans. Curr Protoc Microbiol. (Invited submission & cover image)
  • Sukumaran, A., Coish, J., Yeung, J., Muselius, B., Gadjeva, M., MacNeil, A.J., Geddes-McAlister, J.* (2019). Decoding communication patterns of the innate immune system by quantitative proteomics. J Leukocyte Biol.
  • Geddes-McAlister, J.*, Gadjeva, M. (2019). Mass spectrometry-based quantitative proteomics of murine-derived polymorphonuclear neutrophils. Current Protocols in Immunology.
  • Ball, B., Bermas, A., Carruthers-Lay, D., Geddes-McAlister, J.* (2019) Mass Spectrometry-Based Proteomics of Fungal Pathogenesis, Host–Fungal Interactions, and Antifungal Development. J Fungi.
  • Rea, K.A., Casaretoo, J.A., Al-Abdul-Wahid, M.S., Sukumaran, A., Geddes-McAlister, J., Rothstein, S.J., Akhtar, T.A. (2019). Biosynthesis of cannflavins A and B from Cannabis sativa L. Phytochemistry.
  • Kugadas A., Geddes-McAlister, J., Guy, E., DiGiandomenico, A., Sykes, D.B., Mansour, M.K., Mirchev, R., Gadjeva, M. (2019). Employing enzymatic treatment options for management of ocular biofilm-based infections. J Leukocyte Biol.

2017 & 2018

  • Geddes-McAlister, J., Shapiro, R.S. (2018) New pathogens, new tricks: emerging, drug-resistant fungal pathogens and future prospects for antifungal therapeutics. Antimicrobial Therapeutics Reviews, Annals of the New York Academy of Sciences.
  • Kugadas A., Wright Q., Geddes-McAlister, J., Gadjeva, M. (2017) Role of microbiota in strengthening ocular mucosal barrier function through secretory IgA. Investigative Ophthalmology & Visual Science.

2015 & 2016

  • Geddes, J.M.H., Caza, M., Croll, D., Stoynov, N., Foster, L.J., Kronstad, J.W. (2016) The ubiquitin-proteasome pathway in Cryptococcus neoformans influences capsule production, is regulated by protein kinase A, and is an antifungal target. MBio.
  • Geddes, J.M.H., Croll, D., Caza, M., Stoynov, N., Foster, L.J., Kronstad, J.W. (2015) Secretome profiling of Cryptococcus neoformans reveals regulation of a subset of virulence-associated proteins and potential biomarkers by Protein Kinase A. BMC Microbiol.

2012 & earlier

  • Kronstad, J.W., Saikia, S., Nielson, E.D., Kretschmer, M., Jung, W., Hu, G., Geddes, J.M.H., Griffiths, E.J., Choi, J., Cadieux, B., Caza, M., Attarian, R. (2012) Adaptation of Cryptococcus neoformans to mammalian hosts: integrated regulation of metabolism and virulence. Eukaryotic Cell.
  • Kronstad, J.W., Attarian, R., Cadieux, B., Choi, J., D’Souza, C.A., Geddes, J.M.H., Griffiths, E.J., Hu, G., Jung, W., Kretschmer, M., Saikia, S., Wang, J. (2011) Expanding fungal pathogenesis: Cryptococcus breaks out of the opportunistic box. Nature Reviews Microbiology.
  • Geddes, J.M.H., Eudes, F., Laroche, A., Selinger, L. B. (2008) Differential expression of proteins in response to the interaction between the pathogen Fusarium graminearum and its host, Hordeum vulgare. Proteomics.
  • Geddes, J.M.H., Eudes, F., Tucker, J. R., Legge, W. G., Selinger, L.B. (2008) Evaluation of inoculation methods on Fusarium graminearum infection and deoxynivalenol production in barley. Canadian Journal of Plant Pathology.

Graduate Students:

  • Arjun Sukumaran (PhD)
  • Brianna Ball (PhD)
  • Nicholas Prudhomme (PhD)
  • Arianne Bermas (MSc)
  • Benjamin Muselius (MSc)
  • Jarod Morgenroth-Rebin (MSc)
  • Lilianne Gee (MSc) (Co-supervised with Dr. Jonathan Newman, Laurier University)
  • Jacqueline Thomson (PhD) (Co-supervised with Dr. Jonathan Newman, Laurier University)
  • Boyan Liu (MSc) (Co-supervised with Dr. Rebecca Shapiro, U of Guelph)
  • Reid Buchanan (MSc)

Undergraduate Project:

  • Taylor Ross
  • Morgan Langille
  • Edison Zheng
  • Jacob Hamilton-Rowe
  • Leah Horlings
  • Danisha Johal
  • Marie Pinto
  • Charmaine Retanal
  • Natalie Pagdiwala

Undergraduate Students:

  • Effie Honeywell
  • Daniela Samuel

Research Technician:

  • Samanta Pladwig

Previous Members:

  • Duncan Carruthers-Lay
  • Ammar Afaq
  • Rebecca Stevens-Green
  • Nadolina Brajuka
  • Elizabeth Woroszchuk
  • Jason Yeung
  • Nakshdeep Grewal
  • Sagal Dirie

MICRO*3230 Immunology