Dr. Jennifer Geddes-McAlister

Dr. Jennifer Geddes-McAlister
Assistant Professor
Department of Molecular and Cellular Biology
Email: 
jgeddesm@uoguelph.ca
Phone number: 
52129
Office: 
SSC 4457
Lab: 
SSC 4405/4406

My keen interest in scientific research and academics began during my undergraduate degree at the University of Lethbridge, where I investigated the impact of fungal pathogens on agricultural development and performed my first proteomics experiments. This amazing experience led to a PhD in the laboratory of Dr. Jim Kronstad at UBC, where I applied quantitative proteomics to comprehensively profile the cellular proteome and secretome of the fungal pathogen, Cryptococcus neoformans. My fascination with mass spectrometry-based proteomics and pathogenesis provided the foundation for a postdoctoral fellowship supported by NSERC and the Alexander von Humboldt Foundation in the laboratory of Prof. Dr. Matthias Mann at the Max Planck Institute of Biochemistry in Munich, Germany. During my postdoc, I combined my expertise in microbiology and proteomics with experimental systems immunology to profile the interplay between Salmonella and its host during infection. At the University of Guelph, my research group will blend cutting-edge proteomics technologies with a systems biology perspective to explore host-pathogen interactions.

  • BSc (co-op) – University of Lethbridge, Biological Sciences
  • MSc – University of Lethbridge, Biological Sciences
  • PhD – University of British Columbia, Microbiology and Immunology
  • Postdoctoral Fellow – Max Planck Institute of Biochemistry, Proteomics and Signal Transduction

We are interested in characterizing the mechanisms of pathogenesis, adaptation, and survival in fungal and bacterial microbes from a systems biology perspective through mass spectrometry-based quantitative proteomics. Specifically, research in the lab centres around the following areas:

  1. Differential patterns of pathogenic regulation between microbial species;
  2. Systems biology to elucidate microbial proteome dynamics and interactions;
  3. Mechanistic characterization of pathogenic proteins; and
  4. Mass spectrometry-based proteomics for drug discovery and repurposing.

Our long-term goals emphasize the utilization of mass spectrometry-based proteomics to fundamentally understand differential microbial adaptation and survival strategies and to integrate a novel systems biology platform for investigation of diverse biological systems, defining functional mechanisms of cellular regulation and pathogenesis.

Interested students and post-doctoral fellows are encouraged to contact me to learn more about new and exciting opportunities.

Google Scholar

(*denotes corresponding authorship)

2021

  • Geddes-McAlister, J.*, Sukumaran, A., Vogt, S.L., Rowland, J.L., Woodward, S.E., Muselius, B., Gee, L., Roach, E.J., Khursigara, C.M., Raupach, B., Finlay, B.B., Meissner, F. (In Review). Dual perspective proteomics infectome profiling discovers Salmonella type III secretion system effector functions in macrophages.
  • Liu, B., Stevens-Green, R., Johal, D., Buchanan, R., Geddes-McAlister, J.* (In Review). Fungal pathogens of cereal crops: Proteomic insights into fungal pathogenesis, host defense, and resistance. (Invited submission)
  • Gutierrez-Gongora, D., Geddes-McAlister, J.* (In Review). Realizing the potential of protease inhibitors from environmental sources in biomedical applications against human fungal pathogens.
  • Ince, L.M., Barnoud, C., Lutes, L., Pick, R., Wang, C., Sinturel, F., Chen, C.S., de Juan, A., Weber, J., Holtkamp, S.J., Geddes-McAlister, J., Hergenhan, S.M., Ebner, S., Fontannaz, P.., Meyer, B., Vono, M., Jemelin, S., Dibner, C., Siegrist, C.A., Meissner, F., Graw, F., Scheiermann, C. (In Review) Benefit of circadian clocks in adaptive immunity and vaccination responses.
  • Ball, B., Woroszchuk, E., Sukumaran, A., Afaq, A., Carruthers-Lay, D., Gee, L., Langille, M., Geddes-McAlister, J.* (Accepted – In Revision) Proteomic profiling of zinc limitation in Cryptococcus neoformans reveals regulation of vesicle-associated factors. BMC Microbiology.
  • Meissner, F., Geddes-McAlister, J., Mann, M., Bantscheff, M. (In Press) The emerging role of mass spectrometry-based proteomics in drug discovery. Nature Reviews Drug Discovery. (Invited submission)
  • Goodyear, M.C., Garnier, N., Krieger, J.R., Geddes-McAlister, J.*, Khursigara, C.M.* (In Press) Label-free quantitative proteomics identifies unique proteomes of clinical isolates of the Liverpool Epidemic Strain of Pseudomonas aeruginosa and laboratory strain PAO1.
  • Geddes-McAlister, J.* (In Press) Proteomics in Systems Biology. Methods in Molecular Biology (Book; Invited Editor)
  • Buchanan, R., Serajazari, M., Geddes-McAlister, J.* (In Press) Proteomic profiling of host response in the cereal crop Triticum aestivum to the mycotoxin, 15-acetyldeoxynivalenol, produced by the fungal pathogen, Fusarium graminearum. Methods Mol Biol: Plant-Pathogen Interactions (Invited submission; Book Chapter)
  • Liu, B., Johal, D., Buchanan, R., Ball, B., Serajazari, M., Geddes-McAlister, J.* (In Press) Quantitative phosphoproteome analysis of a cereal crop fungal pathogen’s interaction with the host. Methods Mol Biol: Plant-Pathogen Interactions (Invited submission; Book Chapter)
  • Ball, B., Krieger, J., Geddes-McAlister, J.* (In Press) Phosphoproteomic sample preparation for global phosphorylation profiling of a fungal pathogen. Methods Mol Biol: Proteomics in Systems Biology (Invited submission; Book Chapter)
  • Liu, B., Johal, D., Ball, B., Serajazari, M., Geddes-McAlister, J.* (In Press) Label-free quantitative proteomic profiling of Fusarium head blight in wheat. Methods Mol Biol: Proteomics in Systems Biology (Invited submission; Book Chapter)
  • Prudhomme, N., Krieger, J.R., McLean, M., Cossar, D., Geddes-McAlister, J.* (In Press) Proteomic profiling of interplay between Agrobacterium tumefaciens and Nicotiana benthamiana for improved molecular pharming outcomes. Methods Mol Biol: Proteomics in Systems Biology (Invited submission; Book Chapter)
  • Sukumaran, A. Geddes-McAlister, J.* (In Press) Proteomic profiling of the interplay between a bacterial pathogen and host uncovers novel anti-virulence strategies. Methods Mol Biol: Proteomics in Systems Biology. (Invited submission; Book Chapter)
  • Grafinger, O.R., Stirling, T., Geddes-McAlister, J., Coppolino, M.G. (2021) Inhibition of b1 integrin induces its association with MT1-MMP and decreases MT1-MMP internalization and cellular invasiveness. Cellular Signalling.
  • Sukumaran, A., Pladwig, S., Geddes-McAlister, J.* (2021) Zinc limitation in Klebsiella pneumoniae profiled by quantitative proteomics influences transcriptional regulation and cation transporter-associated capsule production. BMC Microbiol.
  • Retanal, C., Ball, B. & Geddes-McAlister, J.* (2021) Post-translational modifications drive success and failure of fungal-host interactions. J of Fungi (Invited submission)
  • Rosiana, S., Zhang, L., Kim, GH., Retovich, AV., Sukumaran, A., Geddes-McAlister, J., Kirienko, NV., Shapiro, RS. (2021) Systematic characterization and genetic interaction analysis of adhesins in Candida albicans virulence. Genetics.
  • Prudhomme, N., Gianetto-Hill, C., Pastora, R., Cheung, W.-F., Allen-Vercoe, E., McLean, M.D., Cossar, D., Geddes-McAlister, J.* (2021) Quantitative proteomic profiling of shake flask versus bioreactor growth reveals distinct responses of Agrobacterium tumefaciens for preparation in molecular pharming. Can. J. Microbiol.
  • Prudhomme, N., Pastora, R., Muselius, B., McLean, M.D., Cossar, D., Geddes-McAlister, J.* (2021) Exposure of Agrobacterium tumefaciens to agroinfiltration medium demonstrates cellular remodeling and may promote enhanced adaptability for molecular pharming. Can. J. Microbiol.

2020

  • Tremblay, O., Thow, Z., Geddes-McAlister, J., A. Rod Merrill. (2020) Several New Putative Bacterial ADP-Ribosyltransferase Toxins Are Revealed from In Silico Data Mining, Including the Novel Toxin Vorin, Encoded by the Fire Blight Pathogen Erwinia amylovora. Toxins.
  • Geddes-McAlister, J.*, Sukumaran, A., Patchett, A., Hager, H.A., Dale, J.C.M., Roloson, J.L., Prudhomme, N., Bolton, K., Muselius, B., Powers, J., Newman, J.A. (2020) Examining the impacts of CO2 concentration and genetic incompatibility on perennial ryegrass-Epichloee festucae var lolii interactions. J of Fungi.
  • Van Gelder, K., Virta, L., Easlick, J., Prudhomme, N., McAlister, J., Geddes-McAlister, J., Akhtar, T. (2020) A central role for polyprenol reductase in plant dolichol biosynthesis. Plant Sci.
  • Sukumaran, A., Woroszchuk, E., Ross, T., Geddes-McAlister,  J.* (2020) Proteomics of host-bacterial interactions: new insights from dual perspectives. Can. J. Microbiol. (Invited submission).
  • Ball, B., Sukumaran, A., Geddes-McAlister,  J.* (2020) Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions. J Visual Experiments. (Invited submission).
  • Ball, B., Langille, M., Geddes-McAlister,  J.* (2020) Fun(gi)OMICS: advanced and diverse technologies to explore emerging fungal pathogens. MBio. (Invited submission)
  • Bermas, A. and Geddes-McAlister,  J.* (2020) Experimental evolution of antifungal resistance in Cryptococcus neoformans. Curr Prot Microbiol. (Invited submission)
  • Yeung, J., Lamb, J., Krieger, J., Gadjeva, M., Geddes-McAlister,  J.* (2020) Quantitative proteomic profiling of murine ocular tissue and the extracellular environment. Curr Prot Mouse Biol. (Invited submission, cover image)
  • Geddes-McAlister, J.* (2020) Pathogenesis of Fungal and Bacterial Microbes. Pathogens. (Editorial; Invited submission)
  • Horianopoulos, L., Gluck-Thaler, L., Benoit Gelber, I., Cowen, L., Geddes-McAlister, J., Landry, C., Schwartz, I., Scott, J., Sellam, A., Sheppard, D., Spribille, T., Subramaniam, R., Walker, A., Harris, S., Shapiro, R., Gerstein, A. (2020) The Canadian Fungal Research Network: current challenges and future opportunities. Can. J. Microbiol.
  • Bermas, A., Geddes-McAlister, J.* (2020) Combatting the evolution of antifungal resistance in Cryptococcus neoformans. Mol Micro. (Invited submission)
  • Muselius, B.1, Sukumaran, A.1, Yeung, J., Geddes-McAlister, J.* (2020) Iron limitation in Klebsiella pneumoniae defines new roles for Lon protease in homeostasis and degradation by quantitative proteomics. Front Microbiol. 1Authors contributed equally.
  • Anderson, E.M., Sychantha, D., Brewer, D., Clarke, A.J., Geddes-McAlister, J.*, Khursigara, C.M.* (2020) Peptidoglycomics: Examining compositional changes in peptidoglycan between biofilm- and planktonic-derived Pseudomonas aeruginosaJ Biol Chem.
  • Yeung, J., Gadjeva, M., Geddes-McAlister, J.* (2020) Label-free quantitative proteomics distinguishes general -and site-specific host responses to Pseudomonas aeruginosa infection at the ocular surface. Proteomics.

2019

  • Geddes-McAlister, J., Kugadas, A., Gadjeva, M. (2019) Tasked with a challenging objective: why do neutrophils fail to battle Pseudomonas aeruginosa biofilms? Pathogens. (Invited submission)
  • Ball, B., Geddes-McAlister, J.* (2019) Quantitative proteomic profiling of Cryptococcus neoformans. Curr Protoc Microbiol. (Invited submission & cover image)
  • Sukumaran, A., Coish, J., Yeung, J., Muselius, B., Gadjeva, M., MacNeil, A.J., Geddes-McAlister, J.* (2019). Decoding communication patterns of the innate immune system by quantitative proteomics. J Leukocyte Biol.
  • Geddes-McAlister, J.*, Gadjeva, M. (2019). Mass spectrometry-based quantitative proteomics of murine-derived polymorphonuclear neutrophils. Current Protocols in Immunology.
  • Ball, B., Bermas, A., Carruthers-Lay, D., Geddes-McAlister, J.* (2019) Mass Spectrometry-Based Proteomics of Fungal Pathogenesis, Host–Fungal Interactions, and Antifungal Development. J Fungi.
  • Rea, K.A., Casaretoo, J.A., Al-Abdul-Wahid, M.S., Sukumaran, A., Geddes-McAlister, J., Rothstein, S.J., Akhtar, T.A. (2019). Biosynthesis of cannflavins A and B from Cannabis sativa L. Phytochemistry.
  • Kugadas A., Geddes-McAlister, J., Guy, E., DiGiandomenico, A., Sykes, D.B., Mansour, M.K., Mirchev, R., Gadjeva, M. (2019). Employing enzymatic treatment options for management of ocular biofilm-based infections. J Leukocyte Biol.

2017 & 2018

  • Geddes-McAlister, J., Shapiro, R.S. (2018) New pathogens, new tricks: emerging, drug-resistant fungal pathogens and future prospects for antifungal therapeutics. Antimicrobial Therapeutics Reviews, Annals of the New York Academy of Sciences.
  • Kugadas A., Wright Q., Geddes-McAlister, J., Gadjeva, M. (2017) Role of microbiota in strengthening ocular mucosal barrier function through secretory IgA. Investigative Ophthalmology & Visual Science.

2015 & 2016

  • Geddes, J.M.H., Caza, M., Croll, D., Stoynov, N., Foster, L.J., Kronstad, J.W. (2016) The ubiquitin-proteasome pathway in Cryptococcus neoformans influences capsule production, is regulated by protein kinase A, and is an antifungal target. MBio.
  • Geddes, J.M.H., Croll, D., Caza, M., Stoynov, N., Foster, L.J., Kronstad, J.W. (2015) Secretome profiling of Cryptococcus neoformans reveals regulation of a subset of virulence-associated proteins and potential biomarkers by Protein Kinase A. BMC Microbiol.

2012 & earlier

  • Kronstad, J.W., Saikia, S., Nielson, E.D., Kretschmer, M., Jung, W., Hu, G., Geddes, J.M.H., Griffiths, E.J., Choi, J., Cadieux, B., Caza, M., Attarian, R. (2012) Adaptation of Cryptococcus neoformans to mammalian hosts: integrated regulation of metabolism and virulence. Eukaryotic Cell.
  • Kronstad, J.W., Attarian, R., Cadieux, B., Choi, J., D’Souza, C.A., Geddes, J.M.H., Griffiths, E.J., Hu, G., Jung, W., Kretschmer, M., Saikia, S., Wang, J. (2011) Expanding fungal pathogenesis: Cryptococcus breaks out of the opportunistic box. Nature Reviews Microbiology.
  • Geddes, J.M.H., Eudes, F., Laroche, A., Selinger, L. B. (2008) Differential expression of proteins in response to the interaction between the pathogen Fusarium graminearum and its host, Hordeum vulgare. Proteomics.
  • Geddes, J.M.H., Eudes, F., Tucker, J. R., Legge, W. G., Selinger, L.B. (2008) Evaluation of inoculation methods on Fusarium graminearum infection and deoxynivalenol production in barley. Canadian Journal of Plant Pathology.

Graduate Students:

  • Arjun Sukumaran (PhD)
  • Brianna Ball (PhD; EvoFunPath fellow)
  • Nicholas Prudhomme (PhD)
  • Benjamin Muselius (PhD)
  • Boyan Liu (PhD; EvoFunPath fellow) (Co-supervised with Dr. Rebecca Shapiro, U of Guelph)
  • Jacqueline Thomson (PhD) (Co-supervised with Dr. Jonathan Newman, Laurier University)
  • Kerry Woolnough (PBINF) (Co-supervised with Dr. Andrew Hamilton-Wright)
  • Arianne Bermas (MSc)
  • Jarod Morgenroth-Rebin (MSc)
  • Lilianne Gee (MSc) (Co-supervised with Dr. Jonathan Newman, Laurier University)
  • Reid Buchanan (MSc)
  • Michael Woods (MSc)
  • Tia Rizakos (MSc)
  • Davier Gutierrez-Gongora (MSc)
  • Natalie Kim (MSc Bioinformatics) (Co-supervised with Dr. Jeremy Dettman, AAFC-Ottawa)

Undergraduate Project:

  • Kushal Gandhi (4th-year research)
  • Sarah Renaud (4th-year research)
  • Oriana Robinson (4th-year research project)
  • Siobhan O’Connor (4th-year research project)
  • Christian Zanetti (4th-year research project)
  • Thuy Duyen Lisa Tran (4th-year literature review)
  • Dani Samuel (3rd-year internship)
  • Samiha Kazi (Co-op)
  • Payton Curtis (Co-op)
  • Anthony Hendricks (Project student)
  • Effie Honeywell (Project student)

Undergraduate Students:

  • Maggie Cummings (Work study)
  • Hannah West (Work study)
  • Elisa Haley (Work study)

Research Technician:

  • Samanta Pladwig, MSc

Research Associate:

  • Jason McAlister, PhD

Previous Members:

  • Syed Ali (MBINF) (Co-supervised with Dr. Lewis Lukens, U of Guelph)
  • Kerry Woolnough (MBINF) (Co-supervised with Dr. Jasmin Lalonde, U of Guelph)
  • Michael Prudencio-Brunello (4th-year research)
  • Hannah West (Co-op)
  • Duncan Carruthers-Lay (4th-year research)
  • Ammar Afaq (4th-year research)
  • Rebecca Stevens-Green (4th-year research)
  • Nadolina Brajuka (Work study)
  • Elizabeth Woroszchuk (4th-year research)
  • Jason Yeung (4th-year research)
  • Nakshdeep Grewal (4th-year literature)
  • Sagal Dirie (4th-year literature)
  • Morgan Langille (4th-year research)
  • Edison Zheng (4th-year research)
  • Leah Horlings (4th-year research)
  • Marie Pinto (4th-year literature)
  • Charmaine Retanal (4th-year literature)
  • Natalie Pagdiwala (4th-year literature)
  • Taylor Ross (4th-year research)
  • Jacob Hamilton-Rowe (4th-year research)
  • Danisha Johal (4th-year research)
  • Jake Brill (4th-year research)
  • Kristen Haydock (4th-year literature)
  • Shay-Lynn Durand (4th-year literature)
  • Effie Honeywell (Work study)
  • Daniela Samuel (Work study)

MICR*3230 Immunology

MICR*2420 Introduction to Microbiology

MCB*6500/*7500 MSc/PhD Research Topics in MCB

BINF*6890 Topics in Bioinformatics