Final Examination for the Degree of MSc Food Science - Mubashira Zaidi
Date and Time
Location: Webex meeting - by invitation sent from candidate; email candidate for invite
Dr. Yoshi Mine, Chair
Dr. Jeffrey Faber, Advisor
Dr. Sampathkumar Balamurugan, Co-Advisor
Dr. Keith Warriner, Department Member
TITLE: UV RESPONSE OF CRONOBACTER SAKAZAKII IN BUFFER AND PARTLY SKIMMED MILK USING A LOW-PRESSURE COLLIMATED BEAM AND DEAN FLOW-THROUGH REACTOR
In this era of novel and new inactivation techniques in foods, ultraviolet radiation is one of the promising methods. Using UV light for the inactivation of pathogens in food and beverages has shown good results qualitatively, economically, and sustainably. In this research, UV light was used to obtain the D10 values of 13 strains of Cronobacter spp. in buffer. Among the strains, 10 were C. sakazakii, two were C. muytiejensii and one C. dublinensis. The D10 values ranged from 2.08 to 3.14 mJ/cm² (p ≤ 0.05). In the second part of this study, a five-strain cocktail of C.
sakazakii suspended in 2% partly skimmed milk was treated with UV light at different flow rates obtained by using a Dean flow-through reactor. A total reduction of 2.47 ± 0.10 logs was achieved at a flow rate of 10 mL/min. This study provides useful information that can help to establish the efficacy of UV treatment for inactivating Cronobacter spp. in commercial milk.