Final Examination for the Degree of MSc - Lingzi Zong

Date and Time


Food Science lecture room 128


Final Examination for the Degree of MSc

Examining Committee
Dr. Art Hill, Chair
Dr. Yoshi Mine, Advisor
Dr. Paul Spagnuolo, Department Examiner
Dr. Jeff Farber, Department Examiner

TITLE: Effects of probiotic Lactobacillus pentosus S-PT84 and prebiotic isomaltodexrin on microbiota population in low-grade chronic inflammation in C57BL/6 mouse model

ABSTRACT: This project aimed to demonstrate the ability of probiotic Lactobacillus pentosus S-PT84 and prebiotic isomaltodexrin (IMD) on disordered gut microflora in lipopolysaccharide (LPS)-induced low-grade chronic inflammatory mouse model. LPS administration to mice led to increased intestinal permeability and elevated LPS, which ultimately caused metabolic endotoxemia and low-grade inflammation. Real time quantitative polymerase chain reaction (qPCR) was used to determine the quantity change of Bifidobacteria bifidum, Lactobacillus casei, Escherichia coli, Clostridium difficile, and Bacteroides fragilis. The percentage change of each bacterium compared with total bacteria population was calculated to assess the effects of probiotic Lactobacillus pentosus S-PT84 and prebiotic IMD on LPS-induced low-grade inflammatory mice. Probiotic Lactobacillus pentosus S-PT84 and prebiotic IMD had the capacity to alleviate the disturbance of gut microflora in C57BL/6 mice with LPS-induced inflammation as the proportion of Bifidobacteria bifidum, Lactobacillus casei, and Bacteroides fragilis increased, and Escherichia coli and Clostridium difficile decreased. The analysis of short chain fatty acids (SCFA) further supported that the concentration of acetic and butyric acids are positively correlated with
probiotic Lactobacillus pentosus and prebiotic IMD, as well as the percentages of dominant beneficial bacteria. This study elucidates that probiotic Lactobacillus pentosus S-PT84 and prebiotic IMD are beneficial for gut microbiota improvement and potent nutraceuticals.

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