AHL LabNote 36-Clinical sampling for Virology testing at AHL – Sampling supplies
One of the most important aspects of diagnostic work is the actual sampling and handling of the samples prior to being received at the diagnostic laboratory. The Animal Health Laboratory submission instructions are available in our 2015-2016 user manual and on our website.
Usually the best sample for microbiology diagnostics is the substrate itself – feces, tissue, or fluid in leak-proof containers (liquids, feces) or leak-proof “whirl-pak” bags (tissue, feces) (“ziplock” bags are discouraged!)
Bacteriology and Mycoplasmology
AHL prefers a regular gel-based bacteriological swab.
Starswab Amies Clear - VP # 1350500, VWR # CA66410-103L
Or BD single swab - Fisher # B4320115
If swabs are needed, AHL prefers virus transport medium swabs for virology PCR diagnostic tests. For some testing – e.g., as noted in LabNote 30 Porcine PEDV and PDCoV testing at the AHL– a fecal swab in VTM is the preferred sample rather than feces.
Regular gel-based Bacteriology swabs are unsuitable for PCR testing. Dry swabs moistened with sterile saline in a sterile container can be used but VTM is preferred.
BD™ Universal Viral Transport Standard Kit – Fisher Scientific : Catalog # 22 031 15
Or Multitrans™ Collection and Transportation System, Starplex® VWR # CA73270-008
These VTM swabs are also available from AHL for $2.50 – please email firstname.lastname@example.org to order.
Jim Fairles Client Service Veterinarian 519-824-4120 ext 54611 email@example.com