Comprehensive bovine respiratory disease panel - Update

Jim Fairles

Animal Health Laboratory, University of Guelph, Guelph, ON.

AHL Newsletter 2022;26(2):4.

AHL has adjusted this panel to conform to new test combinations available at AHL.  Many practitioners have asked for complete panels as an aid in diagnostic testing.  AHL continues to develop and combine tests to provide more comprehensive diagnostic planning.  The comprehensive bovine diagnostic panel has been developed for this reason, and combines several existing tests and test combinations.

The panel now includes:

1. Bovine viral diarrhea virus/Bovine adenovirus/Bovine coronavirus – triplex PCR (BVDV and adenovirus typing are available on request.)
2. Bovine respiratory virus PCR panel - BoHV-1 (IBR), BPIV-3, BRSV
3. Mycoplasma bovis PCR
4. Bacterial culture, food/fiber producing animals (other than swine).

Samples required:

A) For postmortem samples – lung is the required sample

B) For the live animal, there are four choices:

1. Nasal swab (BacT swab for culture, Virus Transport Media (VTM) swab or dry swab for PCR)
2. Deep pharyngeal swab (normally guarded uterine swab – for bacteriology into gel media; for PCR into VTM or dry swab)
3. Bronchoalveolar lavage (BAL)
4. Transtracheal wash (TTW)

Note: separate samples are needed for both bacteriology (gel media) and PCR (VTM or dry swab).  The swabs cannot be transferred into alternate media upon arrival at the lab.

A reference article (1) outlines a comparison of sample types.  There is good agreement when samples types are compared to TTW except for bovine coronavirus (BCV) and BRSV.  Deep pharyngeal swab appears to be the best non-invasive method for sampling.  It is also important that a representative number of affected animals are sampled to provide a good cross-section of the problem.  The actual number needed is always centered around a representative sample and cost of testing.

This panel is intended for individual animal testing only.  If pooled PCR testing is desired for the virus testing (up to 5 samples), each test must be ordered individually.  Pooled testing is not available for bacterial culture, as these samples are subject to contamination and pooling will amplify this problem.

Reference

1. Doyle D, et al. Agreement among 4 sampling methods to identify respiratory pathogens in dairy calves with acute bovine respiratory disease. J Vet Intern Med 2017;31(3):954–959.

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Check out the other AHL comprehensive test panels:

Bovine neonatal enteric panel                   bentpnl

• includes Bovine coronavirus (BCoV) - PCR, Rotavirus ruminant Group A and B - PCR (rocopcr), Sucrose wet mount (sucwt), Bacterial culture, food/fiber producing animals (other than swine) (cultf, bsetup).
• Feces. Collect and split into 3 leakproof sterile containers.  Refrigerate immediately.

Equine adult diarrhea PCR panel                        adpcrp 

• For adult horses greater than one year of age.
• Includes testing for Salmonella spp., C. perfringens enterotoxin, C. difficile toxin B, Equine coronavirus, Neorickettsia risticii and N. findlayensis.
• 15 g feces. Collect and split into 3 leakproof sterile containers.  Refrigerate immediately.

Equine foal diarrhea PCR panel                        fdpcrp

• For horses less than 1 year of age.
• Includes testing for Salmonella spp., C. perfringens enterotoxin, C. perfringens beta-2, C. perfringens netF, C. difficile toxin B, Lawsonia intracellularis, Rhodococcus equi, Equine coronavirus, Equine rotavirus, Neorickettsia risticii, and N.findlayensis.
• 15 g feces. Collect and split into 3 leakproof sterile containers.  Refrigerate immediately.

We are also glad to discuss any testing questions and concerns.  Please feel free to reach out!

ahlinfo@uoguelph.ca or jfairles@uoguelph.ca; 519-824-4120 extension 54530.